Restricted Expression of Viral Glycoprotein in Vesicular Stomatitis Virus-infected Drosophila melanogaster Cells

Wyers, Françoise; Blondel, Danielle; Petitjean, A M; Dezélée, Sybille

doi:10.1099/0022-1317-70-1-213

Cited by 6 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…N proteins tend to aggregate and their availability in a functional form depends on interactions with P protein58. While there are no studies regarding RNA synthesis in sand fly cells, persistent VSV infection of Drosophila cells results in dramatic changes in the relative amount of mRNA and progeny genomes compared to those generated during replication in mammalian cells59; 60; 61; 62. Production of leader RNA, which is involved in cellular gene expression shut-off, decreases nearly two orders of magnitude, and its transport into the nucleus is inhibited60.…”

Section: Discussionmentioning

confidence: 99%

Antagonistic Pleiotropy Involving Promoter Sequences in a Virus

Presloid

Ebendick-Corpus

Zárate

et al. 2008

Journal of Molecular Biology

View full text Add to dashboard Cite

Selection of specialist genotypes, that is, populations with limited niche width, promotes the maintenance of diversity. Specialization to a particular environment may have a cost in other environments, including fitness tradeoffs. When the tradeoffs are the result of mutations that have a beneficial effect in the selective environment, but a deleterious effect in other environment, we have antagonistic pleiotropy. Alternatively, tradeoffs can result from the fixation of mutations that are neutral in the selective environment but have a negative effect in other environment, and thus the tradeoff is due to mutation accumulation. We tested the mechanisms underlying the fitness tradeoffs observed during adaptation to persistent infection of vesicular stomatitis virus in insect cells by sequencing the full-length genomes of twelve strains with a history of replication in a single niche (acute mammalian infection or persistent insect infection) or in temporally-heterogeneous niches, and correlated genetic and fitness changes. Ecological theory predicts a correlation between the selective environment and the niche width of the evolved populations, such that adaptation to single niches should lead to the selection of specialists and niche cycling should result in the selection of generalists. Contrary to this expectation, adaptation to one of the single niches resulted in a generalist and adaptation to a heterogeneous environment led to the selection of a specialist. Only one-third of the mutations that accumulated during persistent infection had a fitness cost that could be explained in all cases by antagonistic pleiotropy. Mutations involved in fitness tradeoffs included changes in regulatory sequences, particularly at the 3′ termini of the genomes, which contain the single promoter that controls viral transcription and replication.

show abstract

Section: Discussionmentioning

confidence: 99%

Antagonistic Pleiotropy Involving Promoter Sequences in a Virus

Presloid

Ebendick-Corpus

Zárate

et al. 2008

Journal of Molecular Biology

View full text Add to dashboard Cite

show abstract

“…Differential post-translational modification of VSV G by host proteins may prevent its cell membrane localization or incorporation into progeny (Wyers et al, 1989). Following translation, VSV G travels through the endoplasmic reticulum and Golgi apparatus where it undergoes post-translation modification and interacts sequentially with chaperone proteins BiP (GRP78) and calnexin (Hammond and Helenius, 1994) that assist with folding G into its native conformation.…”

Section: Exitmentioning

confidence: 99%

Understanding and altering cell tropism of vesicular stomatitis virus

Hastie¹,

Cataldi²,

Marriott³

et al. 2013

Virus Research

110

116

View full text Add to dashboard Cite

Vesicular stomatitis virus (VSV) is a prototypic nonsegmented negative-strand RNA virus. VSV’s broad cell tropism makes it a popular model virus for many basic research applications. In addition, a lack of preexisting human immunity against VSV, inherent oncotropism and other features make VSV a widely used platform for vaccine and oncolytic vectors. However, VSV’s neurotropism that can result in viral encephalitis in experimental animals needs to be addressed for the use of the virus as a safe vector. Therefore, it is very important to understand the determinants of VSV tropism and develop strategies to alter it. VSV glycoprotein (G) and matrix (M) protein play major roles in its cell tropism. VSV G protein is responsible for VSV broad cell tropism and is often used for pseudotyping other viruses. VSV M affects cell tropism via evasion of antiviral responses, and M mutants can be used to limit cell tropism to cell types defective in interferon signaling. In addition, other VSV proteins and host proteins may function as determinants of VSV cell tropism. Various approaches have been successfully used to alter VSV tropism to benefit basic research and clinically relevant applications.

show abstract

“…Previous experiments have documented the ability of wildtype vesicular stomatitis virus to productively infect mosquito and Drosophila cell lines (12,13 (14).…”

mentioning

confidence: 99%