Restricted access media

Queiroz, Maria Eugênia Costa; Souza, Israel Donizéti de

doi:10.1016/b978-0-12-816906-3.00005-4

Cited by 4 publications

(1 citation statement)

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“…One potential solution to overcome the above‐mentioned challenges of the referred classical chromatography methods is the purification of viruses by mixed‐mode resins using restricted access media (RAM) [15]. Generally, RAM resins combine size exclusion effects of the shell with adsorptive interactions in the core [16]. The shell pores do not allow a passage of larger particles, such as viruses, thus excluding them from entering the core bead.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of restricted access media for the purification of cell culture‐derived Orf viruses

Lothert,

Harsy,

Endres

et al. 2023

Engineering in Life Sciences

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Recently, multimodal chromatography using restricted access media (RAM) for the purification of nanoparticles, such as viruses has regained increasing attention. These chromatography resins combine size exclusion on the particle shell and adsorptive interaction within the core. Accordingly, smaller process‐related impurities, for example, DNA and proteins, can be retained, while larger product viruses can pass unhindered. We evaluated a range of currently available RAM, differing in the shells’ pore cut‐off and the core chemistry, for the purification of a cell culture‐derived clarified model virus, namely the Orf virus (ORFV). We examined impurity depletion and product recovery as relevant criteria for the evaluation of column performance, as well as scale‐up robustness and regeneration potential for evaluating a multiple use application. The results indicate that some columns, for example, the Capto Core, enable both a high DNA and protein removal, while others, for example, the Monomix Core 60 (MC60), are more suitable for DNA depletion. Furthermore, column regeneration is facilitated by using columns with larger shell pores (5000 vs. 700 kDa) and weaker binding interactions (anion exchange vs. multimodal). According to these findings, the choice of RAM resins should be selected according to the respective feed sample composition and the planned number of application cycles.

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Section: Introductionmentioning

confidence: 99%