Restrained MSUM crystallization via hydrogel composited membrane based platform for gout prevention and control

Meng, Yingshuang; Qi, Zhibo; Jiang, Helong; Li, Zhonghua; Xiao, Qianying; Xia, Zeqiu; Yu, M. Y.; Ruan, Xuehua; He, Gaohong; Jiang, Xiaobin

doi:10.1016/j.cej.2022.138155

Cited by 8 publications

(15 citation statements)

References 55 publications

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“…However, a recent study indicated that acetic acid delays nucleation and reduces the final yield of MSUM crystals under uric acid concentrations of 1.5 mmol L −1 , which is closer to real patients. 73 It was found that low concentrations of Mg 2+ , NH 4 + , Ca 2+ , and K + ions promoted MSUM nucleation, while high concentrations of them inhibited MSUM nucleation. The cluster size was characterized by dynamic light scattering technology.…”

Section: Gout and Sodium Urate Crystalsmentioning

confidence: 95%

Section: Typical Diseases Caused By Pathological Crystallizationmentioning

confidence: 99%

“…Recently, Meng et al 73 used a hydrogel membrane to simulate the perichondrium to design a device that simulates the actual joint fluid flow and the diffusion of body fluid components for studying MSUM crystallization in the human body. The hydrogels with abundant carboxyl groups can delay the appearance of MSUM crystals from 20 hours to 82 hours and reduce the crystal yield.…”

Section: Nanoparticles and Hydrogel Materialsmentioning

confidence: 99%

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Progress in the regulation of pathological crystallization

Li,

Meng,

et al. 2023

CrystEngComm

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Section: Gout and Sodium Urate Crystalsmentioning

confidence: 95%

Section: Typical Diseases Caused By Pathological Crystallizationmentioning

confidence: 99%

Section: Nanoparticles and Hydrogel Materialsmentioning

confidence: 99%

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Progress in the regulation of pathological crystallization

Li,

Meng,

et al. 2023

CrystEngComm

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“…The UA concentration used in this experiment was 1.5 mM, which was closer to the patient's blood UA level (0.42−1.0 mM) than the values reported in other literature. 10 The inhibition performances of AA X -HNPs were further assessed by increasing the concentration of UA from 1.5 to 2 mM. As shown in Figures S2 and S3C, the emergence time of large amounts of MSUM was delayed in the presence of AA X -HNPs, but there were explosive nucleation phenomena after a period of time (except for AA 20 -HNPs).…”

Section: Synthesis Of Hcm and Hnps With High Electronegativitymentioning

confidence: 99%

Hydrogel Nanoparticles Enable Nucleation Barrier Regulation and Ion Anchoring as an Alternative Pathway for Monosodium Urate Monohydrate Crystallization Control

Meng,

Wang,

Zhao

et al. 2024

ACS Nano

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Gout flare-up, commonly resulting from monosodium urate monohydrate (MSUM) crystallization, has led to painful inflammatory arthritis among hundreds of millions of people. Herein, a kind of hydrogel nanoparticles (HNPs) with specific properties was developed, aimed at providing a promising pathway for MSUM crystallization control. The experimental and molecular dynamics simulation results synchronously indicate that the fabricated HNPs achieve efficient inhibition of MSUM crystallization governed by the mechanism of “host–guest interaction” even under very low-dose administration. HNPs as the host dispersed in the hyperuricemic model effectively lift the relative heterogeneous nucleation barrier of the MSUM crystal and hinder solute aggregation with strong electronegativity and hydrophobicity. The initial appearance of MSUM crystals was then delayed from 94 to 334 h. HNPs as the guest on the surface of the formed crystal can decelerate the growth rate by anchoring ions and occupying the active sites on the surface, and the terminal yield of the MSUM crystal declined to less than 1% of the control group. The good biocompatibility of HNPs (cell viability > 94%) renders it possible for future clinical applications. This study can guide the rational design of inhibitory nanomaterials and the development of their application in the control of relevant pathological crystallization.

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“…Gout arises from a high uric acid (UA) concentration due to abnormal purine metabolism, frequently manifested as acute gouty arthritis and hyperuricemia. − Facilitated by certain physiological conditions, elevated levels of UA precipitate via in vivo crystallization, forming sodium urate or other urate salt crystals . Among different types of urate crystals, sodium urate is the most prevalent in humans. , The deposition of these insoluble, acicular crystals within joints and tissues triggers the development of acute gouty arthritis. − Currently, pharmacological interventions available for gout management fail to efficiently degrade UA or impede the crystallization of sodium urate, and they often present adverse side effects. , Patients with severe symptoms may require surgical interventions, although such measures do not preclude recurrence …”

Section: Introductionmentioning

confidence: 99%

Adsorption of DNA and Aptamers to Sodium Urate Crystals and Inhibition of Crystal Growth

Yang,

Liu,

Wong

et al. 2024

Langmuir

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An elevated level of blood uric acid (UA) can cause the formation of kidney stones, gout, and other diseases. We recently isolated a few DNA aptamers that can selectively bind to UA. In this work, we investigated the adsorption of a UA aptamer and random sequence DNA onto sodium urate crystals. Both DNA strands adsorbed similarly to urate crystals. In addition, both the UA aptamer and random DNA can inhibit the growth of urate crystals, suggesting a nonspecific adsorption mechanism rather than specific aptamer binding. In the presence of 500 nM DNA, the growth of needle-like sodium urate crystals was inhibited, and the crystals appeared granular after 6 h. To understand the mechanism of DNA adsorption, a few chemicals were added to desorb DNA. DNA bases contributed more to the adsorption than the phosphate backbone. Surfactants induced significant DNA desorption. Finally, DNA could also be adsorbed onto real UA kidney stones. This study provides essential insights into the interactions between DNA oligonucleotides and urate crystals, including the inhibition of growth and interface effects of DNA on sodium urate crystals.

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Restrained MSUM crystallization via hydrogel composited membrane based platform for gout prevention and control

Cited by 8 publications

References 55 publications

Progress in the regulation of pathological crystallization

Progress in the regulation of pathological crystallization

Hydrogel Nanoparticles Enable Nucleation Barrier Regulation and Ion Anchoring as an Alternative Pathway for Monosodium Urate Monohydrate Crystallization Control

Adsorption of DNA and Aptamers to Sodium Urate Crystals and Inhibition of Crystal Growth

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