Restorative effect and mechanism of mecobalamin on sciatic nerve crush injury in mice

Gan, Lin; Minquan, Qian; Shi, Keqin; Chen, Gang; Gu, Yanglin; Du, Wei; Zhu, Guoxing

doi:10.4103/1673-5374.145379

Cited by 26 publications

(10 citation statements)

References 42 publications

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“…Similar results have been obtained by Gan et al who proved a dose-dependent increase of myelin thickness in mice. After the left sciatic nerve of the mice was surgically cut, the animals were subcutaneously treated with vitamin B12 in phosphate-buffered saline (PBS) or only PBS for 12 weeks [47]. In this study, the myelin sheath in regenerated myelinated nerve fibers was significantly thicker in high-dose vitamin B12-treated animals than in the placebo/saline group [47].…”

Section: Yesmentioning

confidence: 77%

The Role of Neurotropic B Vitamins in Nerve Regeneration

Baltrusch

2021

BioMed Research International

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Damage and regeneration naturally occur in the peripheral nervous system. The neurotropic B vitamins thiamine (B1), pyridoxine (B6), and cobalamin (B12) are key players, which maintain the neuronal viability in different ways. Firstly, they constantly protect nerves against damaging environmental influences. While vitamin B1 acts as a site-directed antioxidant, vitamin B6 balances nerve metabolism, and vitamin B12 maintains myelin sheaths. However, nerve injury occurs at times, because of an imbalance between protective factors and accumulating stress and noxae. This will result in the so-called Wallerian degeneration process. The presence of vitamins B1, B6, and B12 paves the way out to the following important regeneration by supporting the development of new cell structures. Furthermore, vitamin B1 facilitates the usage of carbohydrates for energy production, whereas vitamin B12 promotes nerve cell survival and remyelination. Absence of these vitamins will favor permanent nerve degeneration and pain, eventually leading to peripheral neuropathy.

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Section: Yesmentioning

confidence: 77%

The Role of Neurotropic B Vitamins in Nerve Regeneration

Baltrusch

2021

BioMed Research International

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“…A VitB 12 deficiency expresses itself by a wide variety of neurological manifestations such as paraesthesias, skin numbness, coordination disorders and reduced nerve conduction velocity 32 . In the clinic, it is often used to accelerate recovery of peripheral nerves 33 . So, we could find the phenomenon that TQ showed an equal effect as Vitamin B 12 or even better.…”

Section: Discussionmentioning

confidence: 99%

Thymoquinone Alleviates the Experimental Diabetic Peripheral Neuropathy by Modulation of Inflammation

Chen

et al. 2016

Sci Rep

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Thymoquinone has been reported to exhibit antioxidant and anti-inflammatory effects. Inflammation plays an important role in pathogenesis of diabetic peripheral neuropathy. This study investigated the effects of TQ on proliferation and apoptosis of Schwann cells exposed to high glucose conditions and electrophysiological and morphological changes of the sciatic nerve in a DPN rat model as well as relevant inflammatory mechanism. Cell proliferation and apoptosis of Schwann cells were measured using the Cell Counting Kit-8 and flow cytometry. DPN model was established in streptozotocin-induced diabetic rats. Nerve conduction velocity was measured before and after treatment. Morphologic changes were observed by H&E staining and transmission electron microscopy. COX-2, IL-1β, IL-6, and Caspase-3 expression was investigated by western blotting and Bio-Plex ProTM Assays. Finally, TQ alleviated the inhibition of Schwann cell proliferation and protected against Schwann cell apoptosis. It improved nerve conduction velocity, and alleviated the DPN-induced morphological changes and demyelination of the sciatic nerve. COX-2, IL-1β, IL-6 and Caspase-3 expression in sciatic nerve or isolated cultured Schwann cells, were also decreased by TQ. These results indicate TQ has a protective effect on peripheral nerves in a DPN rat model. The mechanism may be mediated partly by the modulation of the inflammatory reaction.

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“…It is a kind of endogenous vitamin B12, which plays a significant role in the reaction process of methionine synthesis [7]- [10]. And Mecobalamin easily enters into neuronal organelles and participates in the synthesis of thymidine, as well as promote the synthesis of protein, enhances the use of folic acid and the metabolism of nucleic acid.…”

Section: Discussionmentioning

confidence: 99%

A Clinical Study on the Treatment of Peripheral Nerve Injury Growth Factor of Mecobalamin Combined with Nerve

Huang¹,

Su²,

Wu³

et al. 2016

WJNS

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Objective: This research aims to explore the clinical curative effect for the treatment peripheral nerve injury growth factor of Mecobalamin combined with nerve. Methods: 150 cases of patients with peripheral nerve injury treated in the hospital in July were selected from 2011 to 2013. Those patients were randomly divided into three groups according to the types of injured nerve and the undergoing treatment order. 50 cases are for each group. Patients in group A were injected with 0.5 mg Mecobalamin by intravenous injection. It lasted for once a day, for 10 days. Later, 0.5 mg Mecobalamin was changed to be taken orally, three times a day, a course of treatment. And a course is 3 to 6 weeks. 50 patients in group B were injected with nerve growth factor for 30 micrograms by intramuscular injection, once a day, a course of treatment. And a course is 3 to 6 weeks. While in group C, 50 patients were treated with Mecobalamin (0.5 mg, intravenous injection, once a day) combined with nerve growth factor (30 mg, intramuscular injection, once a day). A course lasted 3 to 6 weeks. The purpose is to observe the therapeutic effect of two different drugs on patients with peripheral nerve injury. Results: The curative effect of Mecobalamin combined with nerve growth factors for the treatment of peripheral nerve injury in group C is more significant than the single drug treatment in group A and group B. Peripheral nerve injury after sensorimotor function rating evaluation of curative effect made by The British Medical Research Institute of Neurotrauma Society was applied in this research. The comparison of the results of 3 groups after treatment is showed as follows. As to the sensory recovery above level II, the curative * The first author. # Contribute equally as the first author and corresponding author. C. K. Huang et al.76 effect in C group is generally better than in group A and group B, the approximate chi square test H = 13.6573, P = 0.0011. About motion recovery situation, there is a statistical significance in group A and group C which recovered V level X 2 = 3.8431, P = 0.0499, while there is no obvious difference in group B (P > 0.05). Conclusion: Some certain curative effect is presented of Mecobalamin combined with nerve growth factor in the treatment of peripheral nerve injury, which has an important clinical significance. Therefore, it is worth taking into application.

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Restorative effect and mechanism of mecobalamin on sciatic nerve crush injury in mice

Cited by 26 publications

References 42 publications

The Role of Neurotropic B Vitamins in Nerve Regeneration

The Role of Neurotropic B Vitamins in Nerve Regeneration

Thymoquinone Alleviates the Experimental Diabetic Peripheral Neuropathy by Modulation of Inflammation

A Clinical Study on the Treatment of Peripheral Nerve Injury Growth Factor of Mecobalamin Combined with Nerve

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