Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementation

Frez, Flávia Cristina Vieira; Perles, Juliana Vanessa Martins Colombo; Linden, David R.; Gibbons, Simon J.; Martins, Heber Amilcar; Romualdo, Débora Almeida Brito; Souza, Sara Raquel de; Bossolani, Gleison Daion Piovezana; Zanoni, Jacqueline Nelisis

doi:10.17235/reed.2016.4338/2016

Cited by 5 publications

(5 citation statements)

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“…In addition, quercetin exerts protective effects on cellular components of ENS (e.g. enteric glial cells and enteric neurons) and interstitial cells of Cajal by prevention of cellular loss (29,32,33,43) and from the results obtained in this work, regarding 5-HT-IR epithelial cells, quercetin supplementation was able to slightly prevent cell apoptosis induced by diabetes.…”

Section: Discussionsupporting

confidence: 57%

Quercetin Supplementation Prevents Changes in the Serotonin and Caspase-3 Immunoreactive Cells of the Jejunum of Diabetic Rats

Martins-Perles

Zignani

Souza

et al. 2019

Arq. Gastroenterol.

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BACKGROUND: Serotonin (5-HT) is present in the epithelial enterochromaffin cells (EC), mast cells of the lamina propria and enteric neurons. The 5-HT is involved in regulating motility, secretion, gut sensation, immune system and inflammation. OBJECTIVE: Evaluate the effects of diabetes and quercetin supplementation on serotoninergic cells and its cell loss by apoptosis in jejunal mucosa of streptozotocin-induced diabetic rats (STZ-rats). METHODS: Twenty-four male Wistar rats were divided into four groups: normoglycemic (C), normoglycemic supplemented with 40 mg/day quercetin (Q), diabetic (D) and diabetic supplemented with 40 mg/day quercetin (DQ). After 120 days, the jejunum was collected and fixated in Zamboni’s solution for 18 h. After obtaining cryosections, immunohistochemistry was performed to label 5-HT and caspase-3. Quantification of 5-HT and caspase-3 immunoreactive (IR) cells in the lamina propria, villi and crypts were performed. RESULTS: The diabetic condition displayed an increase of the number of 5-HT-IR cells in villi and crypts, while decreased number of these cells was observed in lamina propria in the jejunum of STZ-rats. In the diabetic animals, an increased density of apoptotic cells in epithelial villi and crypts of the jejunum was observed, whereas a decreased number of caspase-3-IR cells was observed in lamina propria. Possibly, quercetin supplementation slightly suppressed the apoptosis phenomena in the epithelial villi and crypts of the STZ-rats, however the opposite effect was observed on the 5-HT-IR cells of the lamina propria. Quercetin supplementation on healthy animals promoted few changes of serotoninergic function and apoptotic stimuli. CONCLUSION: These results suggest that quercetin supplementation mostly improved the serotonergic function affected by diabetes maybe due to antioxidant and anti-inflammatory properties of quercetin.

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Section: Discussionsupporting

confidence: 57%

Quercetin Supplementation Prevents Changes in the Serotonin and Caspase-3 Immunoreactive Cells of the Jejunum of Diabetic Rats

Martins-Perles

Zignani

Souza

et al. 2019

Arq. Gastroenterol.

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“…The jejunum, i.e., the second part of the small intestine, of male adult Wistar rats (Rattus norvegicus), albius variety (D and TW) and holtzmann rats (AIA) were used in this study. The experimental models were developed according to the works of Frez et al (2017) [ 15 ] (D), Vicentini et al (2017) [ 51 ] (TW) and Souza et al (2011) [ 46 ] (AIA). Variations in the animals’ ages were encountered as well, in every disease investigated here.…”

Section: The Datasetsmentioning

confidence: 99%

“…It can be justified mainly by the possibility of distinct tissue fixations and specific immunostaining responses. More detailed information about these processes and the datasets, such as the induction of the disease, may be found in [ 15 ] (D), [ 51 ] (TW), and [ 46 ] (AIA).…”

Section: The Datasetsmentioning

confidence: 99%

Automatic chronic degenerative diseases identification using enteric nervous system images

Felipe

Zanoni

Sehaber-Sierakowski

et al. 2021

Neural Comput & Applic

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Studies recently accomplished on the Enteric Nervous System have shown that chronic degenerative diseases affect the Enteric Glial Cells (EGC) and, thus, the development of recognition methods able to identify whether or not the EGC are affected by these type of diseases may be helpful in its diagnoses. In this work, we propose the use of pattern recognition and machine learning techniques to evaluate if a given animal EGC image was obtained from a healthy individual or one affect by a chronic degenerative disease. In the proposed approach, we have performed the classification task with handcrafted features and deep learning-based techniques, also known as non-handcrafted features. The handcrafted features were obtained from the textural content of the ECG images using texture descriptors, such as the Local Binary Pattern (LBP). Moreover, the representation learning techniques employed in the approach are based on different Convolutional Neural Network (CNN) architectures, such as AlexNet and VGG16, with and without transfer learning. The complementarity between the handcrafted and non-handcrafted features was also evaluated with late fusion techniques. The datasets of EGC images used in the experiments, which are also contributions of this paper, are composed of three different chronic degenerative diseases: Cancer, Diabetes Mellitus, and Rheumatoid Arthritis. The experimental results, supported by statistical analysis, show that the proposed approach can distinguish healthy cells from the sick ones with a recognition rate of 89.30% (Rheumatoid Arthritis), 98.45% (Cancer), and 95.13% (Diabetes Mellitus), being achieved by combining classifiers obtained on both feature scenarios.

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“…Curcumin, a polyphenol from Curcuma longa (turmeric), increases SCF/c-kit protein levels in stomach tissues and ameliorates diabetic gastroparesis in rats by blocking the development of oxidative stress [ 73 ]. Quercetin, a plant flavonol, restores the density of ICCs in the jejuna of diabetic rats through its antioxidant action [ 74 ]. Disruption of GI motility is one of the complications of diabetic mellitus, and its link to ICCs has also been clearly reported [ 75 ].…”

Section: Effects Of Herbal Products Against Oxidative Stress-induced Icc Impairmentmentioning

confidence: 99%

Interstitial Cells of Cajal: Potential Targets for Functional Dyspepsia Treatment Using Medicinal Natural Products

Joung

Choi²,

Son

2021

Evidence-Based Complementary and Alternative Medicine

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Introduction. The pathophysiology of functional dyspepsia (FD) remains uncertain, but the interstitial cells of Cajal (ICCs), pacemakers that regulate gastrointestinal motility, are garnering attention as key modulators and therapeutic targets in FD. This review comprehensively discusses the involvement of ICCs in the pharmacologic actions of FD and as therapeutic targets for herbal products for FD. Methods. A search of the literature was performed using PubMed by pairing “interstitial cells of Cajal” with “medicinal plant, herbal medicine, phytotherapy, flavonoids, or traditional Chinese medicine (TCM).” Results. From the 55 articles screened in the initial survey, 34 articles met our study criteria. The search results showed that herbal products can directly depolarize ICCs to generate pacemaker potentials and increase the expression of c-kit and stem cell factors, helping to repair ICCs. Under certain pathological conditions, medicinal plants also protect ICCs from oxidative stress and/or inflammation-induced impairment. Two representative herbal decoctions (Banhasasim-tang, 半夏泻心汤, and Yukgunja-tang, 六君子汤) have been shown to modulate ICC functions by both clinical and preclinical data. Conclusion. This review strongly indicates the potential of herbal products to target ICCs and suggests that further ICC-based studies would be promising for the development of FD treatment agents.

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Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementation

Abstract: In STZ-treated diabetic rats, diabetes promoted a reduction in the density of jejunal ICC-MY with no significant effect on ICC-DMP. Supplementation with quercetin (DQ) appeared to protect ICC-MY from depletion in diabetes possibly due to its antioxidant action.

Cited by 5 publications

References 55 publications

Quercetin Supplementation Prevents Changes in the Serotonin and Caspase-3 Immunoreactive Cells of the Jejunum of Diabetic Rats

Quercetin Supplementation Prevents Changes in the Serotonin and Caspase-3 Immunoreactive Cells of the Jejunum of Diabetic Rats

Automatic chronic degenerative diseases identification using enteric nervous system images

Interstitial Cells of Cajal: Potential Targets for Functional Dyspepsia Treatment Using Medicinal Natural Products

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