Restoration of angiogenic capacity of diabetes-insulted mesenchymal stem cells by oxytocin

Kim, Yong Sook; Kwon, Jin-Sook; Hong, Moon Hwa; Kang, Won Yu; Jeong, Hyun‐Ja; Kang, Hyejin; Jeong, Myung Ho

doi:10.1186/1471-2121-14-38

Cited by 38 publications

(28 citation statements)

References 40 publications

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“…Senescence or systemic conditions affect the application of autologous BMCs, and may contribute to the functional decline of stem cells. Several studies reported that diabetes impairs the neovascularization of BMCs 2)11)12)…”

Section: Discussionmentioning

confidence: 99%

Angiopoietin-Like 4 Is Involved in the Poor Angiogenic Potential of High Glucose-Insulted Bone Marrow Stem Cells

et al. 2014

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Background and ObjectivesDiabetes is reported to reduce the function or number of progenitor cells. We compared the gene expression patterns of bone marrow-derived mesenchymal stem cells from diabetic (DM-BMCs) and healthy (non-DM-BMCs) rats and suggested Angiopoietin-like 4 (Angptl4) could be a responsible factor for impaired angiogenesis of DM-BMCs.Subjects and MethodsBMCs were isolated from DM or non-DM rat, and in vitro angiogenesis activity was compared by tube formation assay on Matrigel and complementary deoxyribonucleic acid expression was analyzed by microarray with or without oxytocin treatment. Human BMCs (hBMCs) were treated with high glucose, and were performed polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay. Angptl4 plasmid DNA and micro ribonucleic acid-132 (miR-132) were transfected to immortalized hBMCs.ResultsIn vitro angiogenesis assay showed the impaired tube formation in DM-BMCs, and slightly recovery by oxytocin treatment. Angptl4, an adipokine, was upregulated in DM-BMCs compared to non-DM-BMCs. Oxytocin treatment reduced Angptl4 in DM-BMCs. In hBMCs, overexpression of Angptl4 attenuated the tube formation. In addition to Angptl4, miR-132 was increased by high glucose treatment. Collectively, high glucose resulted in impaired tube formation through miR-132 induction and Angptl4 upregulation in BMCs.ConclusionOur results show that the angiogenic activity of BMCs is impaired by high glucose stress, which would be mediated by Angptl4 and miR-132.

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Section: Discussionmentioning

confidence: 99%

Angiopoietin-Like 4 Is Involved in the Poor Angiogenic Potential of High Glucose-Insulted Bone Marrow Stem Cells

et al. 2014

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“…Noiseux et al speculate that preconditioning of mesenchymal stem cells by oxytocin can be used to improve their therapeutic effect [34]. Kim et al also show the use of oxytocin as a priming reagent in stem cell therapy because of its ability to restore angiogenic capacity of diabetes-insulted mesenchymal stem cells [53]. 1,8…”

Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 99%

Influence of the Type of Delivery, Use of Oxytocin, and Maternal Age on POU5F1 Gene Expression in Stem Cells Derived from Wharton’s Jelly within the Umbilical Cord

Gil-Kulik

Chomik

Krzyżanowski

et al. 2019

Oxidative Medicine and Cellular Longevity

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The paper presents an evaluation of the POU5F1 gene expression in mesenchymal stem cells derived from Wharton’s jelly within the umbilical cord, collected from 36 patients during labor. The study is the first one to show that the expression of POU5F1 in mesenchymal stem cells has been dependent on maternal age, birth order, route of delivery, and use of oxytocin. Our research proves that the POU5F1 gene expression in mesenchymal stem cells decreases with each subsequent pregnancy and delivery. Wharton’s jelly stem cells obtained from younger women and during their first delivery, as well as patients treated with oxytocin, show higher POU5F1 gene expression when compared with the subsequent deliveries. This leads to a conclusion that they are characterized by a lower level of differentiation, which in turn results in their greater plasticity and greater proliferative potential. Probably, they are also clinically more useful.

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“…Diyabetten etkilenmi mezenkimal kök hücrelerin anjiyogenik kapasitesinin iyiletirilmesi oksitosinle gerçekletirilmitir ve otolog kök hücre tedavisinde oksitosinin belirteç olarak kullanılması önerilmitir. [45][46][47] Oksitosinin, enflamasyon ve oksidatif hasar üzerindeki etkileri de çeitli çalımalarda aratırılmıtır. ‹ki farklı aratırma grubu, mesane iskemi-reperfüzyon modelinde artan malondialdehit de¤erinin, histopatolojik skorun, polimorf nüveli lökosit ve mast hücre sayılarının oksitosin tedavisi ile azaldı¤ını ve azalan glutatyon peroksidaz de¤erinin arttı¤ını tespit etmilerdir.…”

Section: H‹potalamo-h‹pof‹zer Portal Dolaım Ve Oks‹tos‹n Salınım Anaunclassified

Potential use in cellular treatment of oxytocin

Sir¹

2015

FNG & Bilim Tıp Dergisi

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Oxytocin is a mammalian peptide hormone consisting of nine amino acids. Oxytocin synthesize supraoptic and paraventricular nucleus of hypothalamus and acts as a neuromodulator in the brain. This neurotransmitter has important roles during birth, after birth and reproductivity. It stimulates contraction of the uterine muscles and facilitates birth. It also has the effect of protecting the brain from hypoxia during birth. Oxytocin regulates the menstrual cycle in females and serves in the genital tract ejaculation of males. Oxytocin reduce repetitive behaviors in social interaction and communication damaging diseases like autism. Nowadays, oxytocin therapy and cellular oxytocin studies have gained popularity. Oxytocin inhibits proliferation of breast cancer and other tumor cells. Oxytocin stimulates the cardiomyogenesis and differentiation of stem cells. It demonstrate protective effect from apoptosis on heart. Oxytocin reduces the inflammation associated with stem cells. It stimulates angiogenesis and repairs myocardial ischemia-reperfusion damage. The advantage of the oxytocin therapies provide cell production by itself in organism and hasn't any side effect.

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Restoration of angiogenic capacity of diabetes-insulted mesenchymal stem cells by oxytocin

Cited by 38 publications

References 40 publications

Angiopoietin-Like 4 Is Involved in the Poor Angiogenic Potential of High Glucose-Insulted Bone Marrow Stem Cells

Angiopoietin-Like 4 Is Involved in the Poor Angiogenic Potential of High Glucose-Insulted Bone Marrow Stem Cells

Influence of the Type of Delivery, Use of Oxytocin, and Maternal Age on POU5F1 Gene Expression in Stem Cells Derived from Wharton’s Jelly within the Umbilical Cord

Potential use in cellular treatment of oxytocin

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