Restart of Lignification in Micropropagated Walnut Shoots Coincides with Rooting Induction

Bisbis, Badia; Kevers, Claire; Crèvecœur, Michèle; Dommès, Jacques; Gaspar, Thomas

doi:10.1023/a:1027360326886

Cited by 18 publications

(16 citation statements)

References 22 publications

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“…Explants for rooting were kept on this induction medium in the dark [48]; (2) expression, lasting four weeks on a medium consisting in DKW salts (0.25×gelled with gellan gum), mixed with coarse vermiculite (1:1.25 v/v) and grown under a 16/8 h (light/dark) photoperiod [35]. The expression analysis of selected genes was monitored along the rooting process and in total, harvested 18 microcuttings at the end of multiplication phase (the 20 th days), the end of the root inductive phase (the 28 th day), after the root initiation (the 35 th day), and during the rooting expressive phase (the 42 nd , 49 th and 56 th days at every 7 th day) and then stored in deep-freezer (-80 °C) until further use [4]. For the morphological analysis, the rooting traits of 50 microcuttings were recorded at the end of the root expressive phase.…”

Section: In Vitro Adventitious Rootingmentioning

confidence: 99%

Developmental role of phenylalanine-ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) genes during adventitious rooting of Juglans regia L. microshoots

Cheniany¹,

Ganjeali²

2016

Acta Biologica Hungarica

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Phenylalanine-ammonia-lyase and cinnamate-4-hydroxylase play important role in the phenylpropanoid pathway, which produces many biologically important secondary metabolites participating in normal plant development. Flavonol quercetin is the main representant of these compounds that has been identified in numerous Juglans spp. In this survey, the developmental expression patterns of PAL and C4H genes during in vitro rooting of two walnut cultivars 'Sunland' and 'Howard' was examined by RT-PCR. To understand the potential role in rooting, the changing pattern of endogenous content of quercetin was also analyzed by HPLC. The 'Sunland' with better capacity to root had more quercetin content during the "inductive phase" of rooting than 'Howard'. In each cultivar, the level of PAL transcripts showed the same behavior with the changing patterns of quercetin during root formation of microshoots. The positive correlation between the changes of quercetin and PAL-mRNA indicated that PAL gene may have an immediate effect on flavonoid pathway metabolites including quercetin. Although the behavioral change of C4H expression was similar in both cultivars during root formation (with significantly more level for 'Howard'), it was not coincide with the changes of quercerin concentrations. Our results showed that C4H function is important for the normal development, but its transcriptional regulation does not correlate with quercetin as an efficient phenolic compound for walnut rhizogenesis.

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Section: In Vitro Adventitious Rootingmentioning

confidence: 99%

Developmental role of phenylalanine-ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) genes during adventitious rooting of Juglans regia L. microshoots

Cheniany¹,

Ganjeali²

2016

Acta Biologica Hungarica

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“…Penelitian induksi akar secara in vitro telah dilakukan pada berbagai tanaman seperti walnut (Bisbis et al, 2003), Eucalyptus sp. (Nourissier & Monteuuis, 2008) dan kina (Riyadi & Tahardi, 2009).…”

Section: Pendahuluanunclassified

Pembentukan akar in vitro planlet kelapa sawit (Elaeis guineensis Jacq.) dalam medium cair dengan penambahan auksin In vitro rooting of oil palm (Elaeis guineensis Jacq.) plantlets in a liquid medium supplemented with auxins

RIYADI¹,

SUMARYONO²

2016

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Auxin affects the growth and development of in vitro plantlets including root induction. An experiment was conducted to determine the combination and concentration of auxin for rooting of oil palm plantlets in liquid medium.Unrooted plantlets of oil palm MK 649 clone with height 6 – 7 cm and 2 – 3 leaves were used as material source. The plantlets were cultured in de Fossard liquid medium. The treatments used were combinations of NAA and IBA at 0, 5,10 and 20 μM. The results show that 10 μM NAA combined with 20 μM IBA gave the highest percentage of rooting of oil palm plantlets (73.3%) in 10 weeks. NAA and IBA concentration influenced significantly rooting percentageand root quality and there was a significant interaction between the two auxins. Root initiation response of oil palm plantlets to NAA was higher than to IBA. The best of oil palm root class which indicates root quality was obtained in a medium with 10 μM NAA + 20 μM IBA. The aerial parts of the plantlets grew well in term of shoot height, leaf number and shoot diameter especially in a medium with 10 μM NAA + 20 μM IBA. AbstrakAuksin berpengaruh terhadap pertumbuhan dan perkembangan planlet in vitro, termasuk terhadap induksi akar. Penelitian ini bertujuan untuk menentukan kombinasi dan konsentrasi auksin yang tepat dalam pembentukan akarplanlet kelapa sawit in vitro dalam medium cair. Bahan yang digunakan berupa planlet kelapa sawit klon MK 649 tanpa akar dengan tinggi 6 – 7 cm dan jumlah daun 2 – 3 helai. Planlet dikulturkan dalam medium de Fossard cair. Perlakuan yang digunakan adalah kombinasi NAA dan IBA dengan konsentrasi 0, 5, 10 dan 20 μM. Hasil penelitian menunjukkan bahwa perlakuan NAA 10 μM dikombinasikan dengan IBA 20 μM menghasilkan persentase pembentukan akar planlet kelapa sawit tertinggi yaitu 73,3% dalam waktu 10 minggu. Konsentrasi NAA dan IBA secara nyata mempengaruhi persentase pembentukan dan kualitas akar serta terdapat interaksi yang nyata antara kedua perlakuan auksin. Respons induksi akar kelapa sawit terhadap NAA lebih tinggi daripada IBA. Kelas akar planlet kelapa sawit terbaik yang menunjukkan kualitas perakaran, juga diperoleh pada NAA 10 μM dan IBA 20 μM. Pertumbuhan dan perkembangan organ bagian atas yang meliputi tinggi tunas, jumlah daun dan diameter tunas menunjukkan peningkatan yang cukup baik terutama pada perlakuan NAA 10 μM + IBA 20 μM.

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“…No obstante, el aprovechamiento comercial de esta tecnología es muy limitada en el género Juglans debido a que, por un lado, las salidas de los programas de mejoramiento están reducidas a pocos genotipos ) y, por el otro, a que los nogales son considerados como altamente recalcitrantes al cultivo in vitro (Bisbis et al 2003, Michler et al 2006, Bosela y Michler 2008). …”

Section: Discussionunclassified

“…Obviando el factor genético, algunos detalles podrían apuntar hacia el estado fisiológico de los microbrotes en el momento de pasar a fase de enraizamiento como la principal causa. Por ejemplo Bisbis et al (2003) señalan que la aplicación exógena de auxinas sirvió como señal para el inicio de la lignificación y que la formación de las raíces requieren del aumento continuo de la acumulación de ésta en los brotes de nogal. Como se analizará en el capítulo 4.1.3.2.3, el enraizamiento parece estar influenciado por la edad del microbrote.…”

Section: Fig 14 Detalles Sobre El Tipo De Explante a Utilizar Durantunclassified

“…Santos Macedo et al (2012) también observaron en explantes de olivo tratados con AIB un aumento significativo en la acumulación de fenoles totales, flavonoides y lignina previo a la iniciación radical. Por su parte, en nogal Persa, se ha determinado que la acumulación de lignina fue menor en la población de brotes que no mostró signos visibles de enraizamiento (Bisbis et al 2003). Al mismo tiempo, la acumulación de lignina está asociada con el envejecimiento.…”

Section: Multiplicación Y Pre-acondicionamiento Para El Enraizamientounclassified

Restart of Lignification in Micropropagated Walnut Shoots Coincides with Rooting Induction

Cited by 18 publications

References 22 publications

Developmental role of phenylalanine-ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) genes during adventitious rooting of Juglans regia L. microshoots

Developmental role of phenylalanine-ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) genes during adventitious rooting of Juglans regia L. microshoots

Pembentukan akar in vitro planlet kelapa sawit (Elaeis guineensis Jacq.) dalam medium cair dengan penambahan auksin In vitro rooting of oil palm (Elaeis guineensis Jacq.) plantlets in a liquid medium supplemented with auxins

Biotecnología forestal aplicada a la producción de madera de nogal

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