Responsiveness of voltage-gated calcium channels in SH-SY5Y human neuroblastoma cells on quasi-three-dimensional micropatterns formed with poly (l-lactic acid)

Wu, Zhongzhi; Wang, Zheng-Wei; Zhang, Liguang; An, Zhixing; Zhong, Dong-Huo; Huang, Qiping; Luo, Meirong; Liao, Yanjian; Jin, Liang; Li, Chen-Zhong; Kisaalita, William S.

doi:10.2147/ijn.s38362

Cited by 11 publications

(6 citation statements)

References 46 publications

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“…Fluorescence of each responsive cell was analyzed over time as a different region of interest relative to background fluorescence. The cells that display a fluorescence increase 1.2 times over basal signal were considered responsive (Wu et al, 2013).…”

Section: Methodsmentioning

confidence: 99%

Substrate stiffness effects on SH‐SY5Y: The dichotomy of morphology and neuronal behavior

et al. 2020

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Like many other cell types, neuroblastoma cells are also known to respond to mechanical cues in their microenvironment in vitro. They were shown to have mechanotransduction pathways, which result in enhanced neuronal morphology on stiff substrates. However, in previous studies, the differentiation process was monitored only by morphological parameters. Motivated by the lack of comprehensive studies that investigate the effects of mechanical cues on neuroblastoma differentiation, we used SH-SY5Y cells differentiated on polyacrylamide (PA) gels as a model. Cells differentiated on the surface of PA hydrogels with three different elastic moduli (0.1, 1, and 50 kPa) were morphologically evaluated and their electrophysiological responsiveness was probed using calcium imaging. Immunodetection of neural marker TUJ1 and p-FAK was used for biochemical characterization. Groups with defined stiffness that are matching and nonmatching to neural tissue extracellular matrix were used to distinguish biomimetic results from other effects. Results show that while cells display morphologies that do not resemble neurons on soft substrates, they are in fact electrophysiologically more responsive and abundant in neuronal marker TUJ1. Our findings suggest that while neuronal differentiation occurs more efficiently in microenvironments mechanically mimicking neural tissue, the SH-SY5Y model demonstrates morphologies that conflict with neuronal behavior under these conditions. These results are expected to contribute considerable input to researchers that use SH-SY5Y as a neuron model.

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Section: Methodsmentioning

confidence: 99%

Substrate stiffness effects on SH‐SY5Y: The dichotomy of morphology and neuronal behavior

et al. 2020

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“…Besides the common matrices, new biomolecules have been explored, such as the bacterial nanocellulose, excreted by Gluconacetobacter xylinus [310], or synthesized materials such as poly (L-lactic acid) PLL [311], photocurable polylactide (PLA) resin [312], poly(DL-lactide-co-glycolide) acid [313], carbon structures [314] or polymeric microspheres [315]. However, the main applicative field, by using these new materials, is not the NBL cancer research, but the neural tissue engineering, thanks to the properties of NBL cells to form neuron-like tissues.…”

Section: Nbl Cells On Scaffoldsmentioning

confidence: 99%

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Pizzimenti¹

2014

J. Pediatr. Oncol.

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A large body of evidence indicates that three dimensional (3D) cancer models are superior to two-dimensional (2D) ones in better representing the in vivo phenomena. Indeed, 3D models allow recapitulating in vitro the in vivo features observed in solid tumors (e.g. cell polarity, cell-cell/cell-matrix interactions, biochemical/metabolic gradients, anchorage-independent growth and hypoxia). Moreover, it is well established that the microenvironment plays a fundamental role in regulating tumor development and behavior, including drug resistance. Thus, innovative models able to mimic this complexity represent attractive tools in cancer research. In this review article, we provide a comprehensive review of the application of 3D culture systems in pediatrics' cancer research. In particular, 3D in vitro/ex vivo models of the most common pediatric tumors, such as leukemias, lymphomas and malignancies of the nervous system, will be considered.

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“…A micropillar array as a type of quasi-three-dimensional cell culture substrate affords a unique biophysical cue to tune cell–material interactions and is a promising platform for biomedical research in drug screening and wound healing. − A quasi-three-dimensional or three-dimensional cellular microenvironment is valuable as a physiological or pathological model for gaining fundamental understandings of biology, diseases, and the more accurate prediction of drug responses. − …”

Section: Introductionmentioning

confidence: 99%

Proliferation of Cells with Severe Nuclear Deformation on a Micropillar Array

et al. 2018

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Cellular responses on a topographic surface are fundamental topics about interfaces and biology. Herein, a poly(lactide-co-glycolide) (PLGA) micropillar array was prepared and found to trigger significant self-deformation of cell nuclei. The time-dependent cell viability and thus cell proliferation was investigated. Despite significant nuclear deformation, all of the examined cell types (Hela, HepG2, MC3T3-E1, and NIH3T3) could survive and proliferate on the micropillar array yet exhibited different proliferation abilities. Compared to the corresponding groups on the smooth surface, the cell proliferation abilities on the micropillar array were decreased for Hela and MC3T3-E1 cells and did not change significantly for HepG2 and NIH3T3 cells. We also found that whether the proliferation ability changed was related to whether the nuclear sizes decreased in the micropillar array, and thus the size deformation of cell nuclei should, besides shape deformation, be taken into consideration in studies of cells on topological surfaces.

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Responsiveness of voltage-gated calcium channels in SH-SY5Y human neuroblastoma cells on quasi-three-dimensional micropatterns formed with poly (l-lactic acid)

Cited by 11 publications

References 46 publications

Substrate stiffness effects on SH‐SY5Y: The dichotomy of morphology and neuronal behavior

Substrate stiffness effects on SH‐SY5Y: The dichotomy of morphology and neuronal behavior

In Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to Pediatric Cancer Research

Proliferation of Cells with Severe Nuclear Deformation on a Micropillar Array

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