Responsive systems for cell sheet detachment

In-depth analysis of switchable glycerol based polymeric coatings for cell sheet engineering

“…These polymers were prepared with a terminal thiol functionality for immobilization on gold surfaces and with a non-reactive methoxy terminus for studies of their thermoresponsive properties in solution, respectively (Scheme 1). End-group modifications were confirmed by 1 H-NMR and 13 C-NMR and molecular weights and distributions (PDIs) were determined by GPC and MALDIToF.…”

“…Thermoresponsive polymer coated cell culture substrates can be applied for mild and non-destructive temperature triggered cell harvesting of adherent cells in vitro [1][2][3]. This technique allows the enzymefree and mild detachment of cells from the surface together with their extracellular matrix (ECM).…”

“…So far, various strategies have been described to covalently or non-covalently immobilize PNIPAM on surfaces for the generation of thermoresponsive cell culture substrates [1,14]. Among them is electron beam polymerization of N-isopropylacrylamide on polystyrene cell culture dishes [2,15].…”

Influence of the polymer structure over self‐assembly and thermo‐responsive properties: The case of PEG‐b‐PCL grafted copolymers via a combination of RAFT and ROP

“…1 Among them, thermoresponsive materials represent a wide polymer-class with applications in various fields such as drug delivery, cell culture, and chromatography. [2][3][4][5] Such polymers vary their affinity with the solvent after a temperature modification exhibiting a critical temperature. This critical temperature is called lower critical solution temperature (LCST) when the polymer becomes insoluble above this value or upper critical solution temperature (UCST) when it becomes soluble above this temperature.…”

“…In fact, the collapse of thermoresponsive shells of the NPs over the core, forming hydrophobic superstructures, leads to an environment in which the lipophilic drug can quickly diffuse (Scheme 2). 2 , 98%), sodium sulfate anhydrous (99%), propionyl chloride (98%), triethylamine (99%), 4-4 0 azobis (cyanovaleric acid) (ACVA, 98%), 4-cyano-4-(phenylcarbonothioylthio)-pentanoic acid (CPA, >97%), and pyrene (99%) were all purchased from Sigma Aldrich and used without further treatment except when specifically noted. All the solvents used were of analytical-grade purity and were purchased from Sigma Aldrich.…”