Responsive Polymers in the Fabrication of Enzyme-Based Biosensors

Aggas, John R.; Guiseppi‐Elie, Anthony

doi:10.1016/b978-0-12-816137-1.00079-9

Cited by 7 publications

(11 citation statements)

References 143 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These types of polymers can maintain the redox activity of the monomers; hence, their conducting nature makes them a favorable candidate for electroanalysis studies. Among these polymers, toluidine blue, a water‐soluble member of the redox dye group, has unique electrochemical and chemical features that make it attractive as an electronic mediator in enzyme‐based biosensors 42 . Although phenazines can be directly electro‐polymerized on the surface of the bare electrode, sometimes, the weak binding between the electrode and the growing phase leads to inhibit the nucleation of the monomers on the electrode surface, and thus, an unstable polymer film may be formed 43 .…”

Section: Resultsmentioning

confidence: 99%

Chemical binding of molecular‐imprinted polymer to biotinilated antibody: Utilization of molecular imprinting polymer as intelligent synthetic biomaterials toward recognition of carcinoma embryonic antigen in human plasma sample

Abdollahiyan

Mohammadzadeh

Hasanzadeh

2021

J of Molecular Recognition

View full text Add to dashboard Cite

In this study, a novel biosensor based on molecular imprinting polymer (MIP) methodology was fabricated toward recognition of carcinoembryonic antigen (CEA). For this purpose, poly (toluidine blue) (PTB) was electropolymerized on the surface of gold electrode in the absence and presence of CEA. So, the target molecules were entrapped into the imprinted specific cavities of MIP. Obtained results show that, the binding affinity of the MIP system was significantly higher than that of revealed for the nonimprinted polymer (NIP) system, MIP‐based biosensor revealed linear response from (0.005 to 75 μg/L) and low limit of quantification of (0.005 μg/L) by using chronoamperometry technique, leading to CEA monitoring in real and clinical samples. Thus, a novel technique for rapid, simple, sensitive and affordable monitoring of CEA (LLOQ = 0.005 μg/L) has provided through developed biosensor. From a future perspective, moreover, this method can be considered as an applicable candidate in biomedical and clinical analysis for point‐of‐care usages.

show abstract

Section: Resultsmentioning

confidence: 99%

Chemical binding of molecular‐imprinted polymer to biotinilated antibody: Utilization of molecular imprinting polymer as intelligent synthetic biomaterials toward recognition of carcinoma embryonic antigen in human plasma sample

Abdollahiyan

Mohammadzadeh

Hasanzadeh

2021

J of Molecular Recognition

View full text Add to dashboard Cite

show abstract

“…Analyte measurement by piezoelectric transducers is obtained by the Sauerbrey's equation (Eq. 1), employing the change in resonant frequency (ΔF), original oscillation frequency (f), mass changes (Δm), area of the coating (A), and a particular constant of the used crystal (K) [82].…”

Section: Transduction Methods Used In Immobilized Enzyme Biosensorsmentioning

confidence: 99%

Immobilized Enzyme-based Novel Biosensing System for Recognition of Toxic Elements in the Aqueous Environment

et al. 2023

View full text Add to dashboard Cite

Access to secure water sources has become one of the biggest challenges for human sustainability. Climate change and associated droughts make it difficult to guarantee the usual water source and move to groundwater use or to the re-use of treated wastewater remains unviable due the lack on the capacity of monitoring water quality. Moreover, reusing treated wastewater from repositories near anthropogenic sources represents a risk of high concentrations of emerging contaminants. The strategies involve a higher risk of encountering toxic elements with a heavy burden on human and environmental health. New accessible and reliable tools are required to detect any hazard from the waterbodies in real time to ensure safe management and also to decrease mismanagement or ilegal water discharges. One of the available options is to look into enzyme-based biosensors that can detect toxic elements in the water. The proposed biosensors require sensible elements to be accessible and durable for their proper function. The present revision shows in first place, the actual need of real time monitoring due the different sources and effects of emergent pollutants. Secondly, describes how enzymes can be immobilized for its application in biosensors and the rol enzymes play as bioreceptor element in biosensing. Thirdly, describes the transduction methods that can be observed, and finally the actual application of enzyme biosensors for the detection of different toxic elements. According to the presented literature enzyme-based biosensors have been successfully applied for the detection of a wide number of pollutants reaching detection limits comparable to traditional methods such as up to 0.018 nM of mercury. Furthermore, laccase seems to be the more applied enzyme in literature, but positive results are not limited to this enzyme and other candidates have been explored showing good detection rate.

show abstract

“…The general classification of CRS is based on the mechanisms that govern the release process and the entrapment of the drug [142]. Thus, the main types of CRS are: (i) diffusion-controlled CRS, which act as reservoirs and monolithic systems; (ii) water penetration-controlled CRS, where osmotic and swelling processes control the release of the drug; (iii) chemically-controlled CRS, which can be designed as biodegradable reservoirs and monolithic systems or biodegradable polymer backbones with pendant drugs, where the release ratio is managed by chemical reactions between the device and the medium; (iv) responsive CRS, which act as physically or chemically responsive systems, mechanical-, magnetic-, or ultrasound-responsive systems, and biochemicallyresponsive self-regulated systems; (v) particulate CRS, which can be designed as microparticles, liposome systems, and polymer-drug conjugates, among others [142].…”

Section: Controlled Drug Delivery Systemsmentioning

confidence: 99%

“…The most important CRS developed for nanomedicine applications are liposomes, micelles, nanoparticles, carbon nanotubes, graphene sheets, hydrogels, dendrimers, polyelectrolyte complex, and quantum dots. Table 2 summarizes the main characteristics of all CRS developed for biomedical applications [141,142].…”

Section: Controlled Drug Delivery Systemsmentioning

confidence: 99%

See 1 more Smart Citation

Nanomedicine-based technologies and novel biomarkers for the diagnosis and treatment of Alzheimer’s disease: from current to future challenges

Cano

Turowski²,

Ettcheto

et al. 2021

J Nanobiotechnol

View full text Add to dashboard Cite

Increasing life expectancy has led to an aging population, which has consequently increased the prevalence of dementia. Alzheimer's disease (AD), the most common form of dementia worldwide, is estimated to make up 50–80% of all cases. AD cases are expected to reach 131 million by 2050, and this increasing prevalence will critically burden economies and health systems in the next decades. There is currently no treatment that can stop or reverse disease progression. In addition, the late diagnosis of AD constitutes a major obstacle to effective disease management. Therefore, improved diagnostic tools and new treatments for AD are urgently needed. In this review, we investigate and describe both well-established and recently discovered AD biomarkers that could potentially be used to detect AD at early stages and allow the monitoring of disease progression. Proteins such as NfL, MMPs, p-tau217, YKL-40, SNAP-25, VCAM-1, and Ng / BACE are some of the most promising biomarkers because of their successful use as diagnostic tools. In addition, we explore the most recent molecular strategies for an AD therapeutic approach and nanomedicine-based technologies, used to both target drugs to the brain and serve as devices for tracking disease progression diagnostic biomarkers. State-of-the-art nanoparticles, such as polymeric, lipid, and metal-based, are being widely investigated for their potential to improve the effectiveness of both conventional drugs and novel compounds for treating AD. The most recent studies on these nanodevices are deeply explained and discussed in this review. Graphic Abstract

show abstract

Responsive Polymers in the Fabrication of Enzyme-Based Biosensors

Cited by 7 publications

References 143 publications

Chemical binding of molecular‐imprinted polymer to biotinilated antibody: Utilization of molecular imprinting polymer as intelligent synthetic biomaterials toward recognition of carcinoma embryonic antigen in human plasma sample

Chemical binding of molecular‐imprinted polymer to biotinilated antibody: Utilization of molecular imprinting polymer as intelligent synthetic biomaterials toward recognition of carcinoma embryonic antigen in human plasma sample

Immobilized Enzyme-based Novel Biosensing System for Recognition of Toxic Elements in the Aqueous Environment

Nanomedicine-based technologies and novel biomarkers for the diagnosis and treatment of Alzheimer’s disease: from current to future challenges

Contact Info

Product

Resources

About