Responses of Smooth Muscle Cells to Platelet-Derived Growth Factor Are Inhibited by Herbimycin-A Tyrosine Kinase Inhibitor

Fujio, Yasushi; Yamada, Fumiko; Takahashi, Kumiko; Shibata, Norihito

doi:10.1006/bbrc.1993.2012

Cited by 22 publications

(8 citation statements)

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“…The differences that we observed in the plasma lipid and lipoprotein concentrations in the two groups among the female animals are summarized in Table I. We have focused on the phytoestrogens of soybeans (SBE) as a potential nutritional alternative based upon the putative protective effect of these compounds against the development of breast cancer (25)(26)(27)(28)(29)(30), the likely lack of a harmful effect on the uterus (31)(32)(33)(34)(35)(36)(37), and an experimental basis for assuming probable favorable effects on coronary artery atherosclerosis, including antioxidant properties (38) and inhibition of smooth muscle cell proliferation (39). Additionally, a nutritional supplement derived from a natural product may be more acceptable than a pharmacologic therapy and thus improve compliance.…”

Section: Rationale For Soybean Estrogensmentioning

confidence: 99%

The Potential of Soybean Phytoestrogens for Postmenopausal Hormone Replacement Therapy

Clarkson

Anthony

Williams

et al. 1998

Experimental Biology and Medicine

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Hormone replacement therapy for postmenopausal women greatly reduces their risk of coronary heart disease. However, current pharmaceutical regimens have a low acceptance rate among postmenopausal women. We have sought to identify an alternative treatment that would retain the beneficial health effects of current standard therapy without its negative aspects. We have concentrated our research on naturally occurring estrogens (called phytoestrogens) found in soybeans, in the belief that delivery of phytoestrogens via the diet would be more acceptable than pharmaceutical regimens. Using a well-established nonhuman primate model of postmenopausal women, we have investigated the effects of soy phytoestrogens on cardiovascular risk factors and the reproductive system. In this review, we summarize the results of our ongoing research.

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Section: Rationale For Soybean Estrogensmentioning

confidence: 99%

The Potential of Soybean Phytoestrogens for Postmenopausal Hormone Replacement Therapy

Clarkson

Anthony

Williams

et al. 1998

Experimental Biology and Medicine

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“…Therefore, the present study used 0.5 µmol/L of staurosporine‐A and 0.1 µg/mL (0.12 µmol/L) of calphostin‐C as PKC inhibitors. Herbimycin‐A at 1.0 µg/mL markedly inhibited PDGF‐induced cell proliferation and tyrosine phosphorylation in vascular smooth muscle cells (15). Treatment of A431 cells with 100 µg/mL of genistein completely prevented the tyrosine phosphorylation of epidermal growth factor‐receptor mediated by epidermal growth factor (16).…”

Section: Methodsmentioning

confidence: 99%

Ascites from Patients with Encapsulating Peritoneal Sclerosis Augments NIH/3T3 Fibroblast Proliferation

et al. 2003

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Encapsulating peritoneal sclerosis (EPS) remains one of the major causes of dropout in continuous ambulatory peritoneal dialysis by reducing ultrafiltration capacity. To demonstrate whether ascites from patients with EPS (EPS ascites) has fibroblast proliferation activity, we used NIH/3T3 fibroblasts to examine the effects of EPS ascites on fibroblast proliferation activity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Encapsulating peritoneal sclerosis ascites dose-dependently augmented NIH/3T3 fibroblast proliferation. The protein kinase C inhibitors and the tyrosine kinase inhibitors partially inhibited the stimulatory effects of EPS ascites on fibroblast proliferation activity. In EPS ascites, levels of interleukin (IL)-1beta, IL-6, IL-8, transforming growth factor (TGF)-beta1, hepatocyte growth factor (HGF), and platelet-derived growth factor (PDGF)-AB were elevated. The treatment with IL-1beta, HGF, TGF-beta1, and PDGF-AB alone or in combination at similar concentrations to those in EPS ascites exhibited small but significant fibroblast proliferation activities. We conclude that EPS ascites stimulate NIH/3T3 fibroblast proliferation via protein kinase C and tyrosine kinase. The elevated cytokine and growth factors partly contribute to the EPS ascites-induced fibroblast proliferation.

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“…Reduction of total and LDL cholesterol and TG; increase HDL cholesterol* [30,31] Increased antioxidant capacity [43] Inhibition of LDL cholesterol oxidation [42] Inhibition of thrombosis [43] Suppression of platelet activation and aggregation [42] Inhibition of smooth muscle cell migration and proliferation [45] Enhanced endothelium-dependent vasodilation [43,44] Hypotensive action (ACE-inhibitory peptides) [54] *The magnitude of potential changes in blood cholesterol is dependent on baseline cholesterol levels. ACE-angiotensin-converting enzyme; HDL-high-density lipoprotein; LDL-low-density lipoprotein; TG-triglycerides.…”

Section: Table 3 Putative Biologic Actions Of Soy Protein On Isoflavmentioning

confidence: 99%

Effects of dietary animal and soy protein on cardiovascular disease risk factors

Hecker

2001

Curr Atheroscler Rep

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A growing body of research offers insight into the influence of dietary protein on cardiovascular disease (CVD) risk. Early studies in rabbits indicated that animal protein was atherogenic; however, this has not been demonstrated in other animals species (pig, primate) or humans. More recent studies have found that low-fat animal protein can effectively improve some CVD risk factors. Soy protein has received much attention in recent years due to its seemingly marked effect on plasma cholesterol levels. However, further research has shown the hypocholesterolemic effect of soy protein to be most pronounced in cases of moderate to severe elevations in plasma lipids. Still, animal and soy protein sources contain a variety of bioactive components that may offer protection above and beyond cholesterol reduction. This review provides evidence that as part of a low-saturated fat diet, animal and/or soy protein intake at or above the recommended intake level of 15% of total calories lowers plasma cholesterol levels, reduces blood pressure, and potentially facilitates healthy weight management.

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Responses of Smooth Muscle Cells to Platelet-Derived Growth Factor Are Inhibited by Herbimycin-A Tyrosine Kinase Inhibitor

Cited by 22 publications

References 0 publications

The Potential of Soybean Phytoestrogens for Postmenopausal Hormone Replacement Therapy

The Potential of Soybean Phytoestrogens for Postmenopausal Hormone Replacement Therapy

Ascites from Patients with Encapsulating Peritoneal Sclerosis Augments NIH/3T3 Fibroblast Proliferation

Effects of dietary animal and soy protein on cardiovascular disease risk factors

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