Responses of photosynthetic properties and antioxidant enzymes in high-yield rice flag leaves to supplemental UV-B radiation during senescence stage

Abstract

Despite the increasing occurrence of ultraviolet-B (UV-B) radiation, its molecular mechanism is poorly documented in higher plants compared to other environmental stress. In present study, the influence of supplemental UV-B radiation on photosynthetic performance and antioxidant enzymes in rice (Oryza sativa L.) was investigated. Supplemental UV-B radiation reduced net photosynthetic rate in rice flag leaves during senescence stage. By means of the JIP-test, it was found that the potential of processing light energy through the photosynthetic machinery was slightly inhibited by the increased thermal dissipation. Furthermore, 18 thylakoid membrane protein spots were differentially expressed (5-fold or greater variation compared to the control) in supplemental UV-B-treated rice. These identified proteins were involved in various cellular responses and metabolic processes including photosynthesis, stress defense, Calvin cycle, and others of unknown functions. Taken together, these results suggested that physiological changes that resulted from supplemental UV-B radiation were linked to the light reaction, carbon metabolism, and antioxidant enzymes in rice leaves during senescence stage.

Effects of UV‐B on vitamin C, phenolics, flavonoids and their related enzyme activities in mung bean sprouts (Vigna radiata)

“…UV-B irradiation is a kind of oxidative stress, which can lead to a series of reactions in plants (Peng & Zhou, 2009). In any case, the UV-B radiation is expected to be adverse on higher plants growth even at relative small amounts, including reduction in biomass accumulation, inhibition of photosynthetic processes, impairment of organelles and formation of oxidative contents in physiological processes (Wang et al, 2015b). However, UV-B stress can increase the content of secondary metabolites, which are very useful for human health (Namli et al, 2014).…”

“…POD activity was assayed following the method described by Wang et al . (,b) with some modifications. The reaction solution contained 3 mL of 25 mmol L −1 guaiacol, 0.2 mL of 0.5 mol L −1 H 2 O 2 and 0.05 mL crude extract.…”

Natural Holobiome Engineering by Using Native Extreme Microbiome to Counteract the Climate Change Effects

“…UV-B has been considered as the most harmful type of radiation for plants, morphological changes have been observed as thicker leaves, shorter petioles and lower chlorophyll content (Zhu et al, 2010;Inostroza-Blancheteau et al, 2014;Robson et al, 2015). Studies conducted in recent years show that high UV-B irradiation can even decrease CO 2 assimilation, decrease photochemical efficiency of photosystem II (PSII), reduce electron transport rate (ETR) and limit the productivity of some crops (Basahi et al, 2014;Wang et al, 2015;González-Villagra et al, 2020). Our current knowledge has determined that plants can counteract the negative effect of UV-B through a series of morphological and molecular changes, such as, the biosynthesis of phenolic acids and flavonoids induced by a UV-B photoreceptor called UV RESISTANCE LOCUS 8 or UVR8 (Coffey et al, 2017;Yang et al, 2018;Kondou et al, 2019;Tossi et al, 2019).…”

Assessment of male sterility and antioxidant enzyme activities induced by the chemical hybridization agent SQ-1 in Foxtail Millet (Setaria italica)

“…Excessive production of ROS causes oxidative injuries to lipid peroxidation as observed under different stress conditions for different plant species (Khoyerdi et al, 2016;Wang et al, 2015). The lipid peroxidation level was indirectly determined by the content of MDA in this study.…”