Responses of periodontal ligament stem cells on various titanium surfaces

Yy, Heo; S, Um; Sk, Kim; Park, Jong; Bm, Seo

doi:10.1111/j.1601-0825.2010.01728.x

Cited by 29 publications

(19 citation statements)

References 16 publications

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“…A stable and sufficiently stiff and resistant interface between the biomaterial surface and the surrounding tissue is a vital prerequisite both for immediate implant loading and for the long-term success of such implants. This phenomenon has been extensively analyzed in the literature [3][4][5][6] . The interface is usually achieved by the biological process (partially regulated by mechanical and material properties) known as osseointegration of the implant, which is obtaining an intimate connection of the implant within the bone by means of an appropriate and sufficient growth of new bone on the surface of the implant 7) .…”

Section: Introductionmentioning

confidence: 99%

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Santander¹,

Alcaine²,

Lyahyai³

et al. 2012

Dent. Mater. J.

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Section: Introductionmentioning

confidence: 99%

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Santander¹,

Alcaine²,

Lyahyai³

et al. 2012

Dent. Mater. J.

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“…A recent study on stem cells derived from the periodontal ligament has reported increased osteogenic differentiation of the cells seeded on rough surfaces coated with Al 2 O 3 particle-treated Ti [31]. Furthermore, another study investigating MC3T3, human bone marrow stromal cells, murine femoral stromal cells, and canine bone marrow cells have reported significant variations in their mineralisation and proliferation response to Ti surfaces [32].…”

Section: Discussionmentioning

confidence: 99%

Effect of TiO2 Nanotube Layers Thickness on Periodontal Ligament Cells

Lv¹

2016

Dentistry

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Periodontal ligament cells (PDLCs) remaining in the tooth-extraction socket are involved in osseointegration after immediate implantation; however, their interaction with different implant surfaces has not been investigated. The aim of this study was to compare PDLC growth on substrates composed of differently sized titanium dioxide (TiO 2 ) nanotubes with that on flat Ti. PDLC growth on Ti nanotubes was evaluated in terms of cell adhesion, proliferation, and osteogenic differentiation based on the expression of alkaline phosphatase (ALP), type 1 collage (COL-1), osteopontin (OPN), and Runt-related transcription factor 2 (RUNX2). We found that TiO 2 nanotubes of different diameters (30, 70, and 120 nm) manufactured on Ti surface by anodisation had a well-defined structure. PDLCs grown on nanotube layers demonstrated polygonal morphology with more filopodia than those on flat Ti, which was especially evident on the nanotubes of larger diameters. However, cell adhesion and proliferation was the highest on the smallest 30 nm nanotubes. Similarly, mRNA levels of the ALP, COL-1, OPN, and RUNX2 genes increased in PDLCs cultured on 30 nm TiO 2 nanotube layers compared to those in the cells grown on 70 nm and 120 nm nanotubes. In conclusion, small diameter (30 nm) nanotube layers can support PDLC adhesion, proliferation, and differentiation better than larger sized nanotubes, and consequently, have higher potential to promote bone formation and integration of an immediate implant.

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“…In addition, they also represent a source of stem cells for regenerative medicine applications, that could be applied around dental implants in areas where there is initially insufficient bone. Initial investigations have shown the potential of utilising PDLSCs to assess osteoblastic differentiation on various titanium surfaces . −7 However, their behavior in contact with bioactive materials such as hydroxyapatite (HA) and associated calcium phosphate (CaP) systems is less well understood.…”

Section: Introductionmentioning

confidence: 99%

Osteoblastic differentiation of periodontal ligament stem cells on non‐stoichiometric calcium phosphate and titanium surfaces

Winning

Robinson

Boyd

et al. 2017

J Biomedical Materials Res

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Bioactive materials offer particular clinical benefits in the field of dental implantology, where differentiation of stem cells towards an osteoblastic lineage is required for osseointegration and appropriate function of implants in vivo. The aim of this study was to evaluate the osteoblastic response of Stro‐1 +ve periodontal ligament stem cells (PDLSCs) to three well‐characterized biomaterial surfaces: an abraded titanium surface (cpTi) control; a polycrystalline titanium surface, with both micro and nanotopography produced by radio frequency magnetron sputtering (TiTi); and the same surface incorporating a sputter deposited calcium phosphate coating (CaP‐TiTi). The CaP‐TiTi surfaces were nonstoichiometric, carbonated, and calcium rich with a Ca/P ratio of 1.74. PDLSCs were grown on each surface in the absence of supplementary osteogneic‐inducing agents. Osteoblastic responses were assessed for up to 21 days in culture by measuring gene expression using real time q‐PCR and via assessment of intracellular alkaline phosphatase (ALP) activity. Gene expression analysis for the CaP‐TiTi surfaces showed a significant late stage up‐regulation of Secreted Phosphoprotein 1. Additionally, there was a significant up‐regulation of the Wnt signaling genes β‐catenin and Wnt Family Member 5 A on days 14 and 21, respectively for the CaP‐TiTi surface. A significant increase in intracellular ALP at day 21 for the CaP‐TiTi surface was also observed. These data suggest that the CaP‐TiTi surfaces provide the bioactive conditions required for direct osteoblastic differentiation of PDLSCs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1692–1702, 2017.

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Responses of periodontal ligament stem cells on various titanium surfaces

Cited by 29 publications

References 16 publications

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

Effect of TiO2 Nanotube Layers Thickness on Periodontal Ligament Cells

Osteoblastic differentiation of periodontal ligament stem cells on non‐stoichiometric calcium phosphate and titanium surfaces

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