Responses of NFR/N inbred mice to very low-dose infections with Ascaris suum

Jeska, Edward L.; Stankiewicz, M.

doi:10.1016/0020-7519(89)90025-8

Cited by 9 publications

(9 citation statements)

References 15 publications

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“…We examined whether vaccination with rAs16 induced protection in a mouse-A. suum model (22,23,49) in order to evaluate the use of rAs16 as a new vaccine candidate for parasitic diseases caused by Ascaris nematodes. Since the mucosal surface of the small intestine is the initial site of the A. suum infection, it is important to establish protective immunity there (23).…”

Section: Figmentioning

confidence: 99%

“…The initial phase of A. suum infection occurs in the mucosal surface of the cecum and/or proximal colon of the host, and this is followed by the tissue migratory phase. It has been shown that local antibodies present at the site where the infective L3 enter the host can induce partial protection against A. suum infection in mice (23). Thus, intestinal immunity should be an important initial defense against the invasion of A. suum larval stages into the host, whereas systemic immunity mediated by serum antibodies may protect the host against larval migration.…”

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confidence: 99%

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Mice Intranasally Immunized with a Recombinant 16-Kilodalton Antigen from RoundwormAscarisParasites Are Protected against Larval Migration ofAscaris suum

et al. 2003

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Protective immunity to the pig roundworm, Ascaris suum, has been demonstrated by immunization of pigs with antigens derived from the parasite's larval stages. We identified a protective antigen commonly expressed in the human and pig Ascaris infections as a 16-kDa protein (As16), which has no similarity at the amino acid level to mammalian proteins but has some similarity to those of the filarial parasites and Caenorhabditis elegans gene product. Localization analysis revealed that the native As16 was highly expressed in the adult worm intestine, hypodermis, and cuticles. In addition, As16 was detected in the parasite excretory and secretory products. Mice intranasally vaccinated with Escherichia coli-expressed recombinant As16 (rAs16), coupled with cholera toxin B subunit, generated a significant increase in the level of rAs16-specific immunoglobulin G (IgG) and IgE in serum. Mucosal IgA levels were also increased. The recombinant protein evoked a mixed (both Th1 and Th2) type of immune response characterized by elevated levels of gamma interferon and interleukin-10 in the culture supernatants of activated spleen cells. An increased level of IgG1 and IgG2a in serum was also observed. The vaccinated mice showed a reduction by 58% in the recovery of challenged larvae compared to a nonvaccinated control. These results suggest the possibility of developing a mucosal vaccine for human and pig ascariasis.

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Section: Figmentioning

confidence: 99%

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confidence: 99%

Mice Intranasally Immunized with a Recombinant 16-Kilodalton Antigen from RoundwormAscarisParasites Are Protected against Larval Migration ofAscaris suum

et al. 2003

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“…Since the mucosal surface of the small intestine is the initial site of the A. suum infection, it is important to establish protective immunity there (27). It has been reported elsewhere that administration of A. suum L3 to animals results in induction of an A. suum L3-specific IgA response in the small intestine (25). However, a major problem with the delivery of antigens to the intestinal mucosa is that oral administration of soluble proteins gives rise either to no immune response or to the development of tolerance (59).…”

Section: Vol 69 2001mentioning

confidence: 99%

“…The initial phase of A. suum infection occurs in the mucosal surface of the small intestine of the host, and this phase is followed by the tissue migratory phase. It has been shown elsewhere that local antibodies present at the site where the L3 enter the host can induce partial protection against A. suum L3 infection in mice (25). Thus, intestinal immunity appears to be an important primary defense against the invasion of A. suum L3 into the host, while systemic immunity mediated by serum antibodies may protect the host against larval migration.…”

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confidence: 99%

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Intranasal Immunization with RecombinantAscaris suum14-Kilodalton Antigen Coupled with Cholera Toxin B Subunit Induces Protective Immunity toA. suumInfection in Mice

et al. 2001

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Animals can be rendered immune to Ascaris parasites by immunization with infectious-stage larvae. The specific parasite gene products that mediate protective responses in ascariasis are unknown. We have identified a cDNA encoding Ascaris suum 14-kDa antigen (As14) and evaluated the vaccinal effect of the Escherichia coli-expressed recombinant protein (rAs14). GenBank analysis showed that As14 has low similarity at the amino acid level to a Caenorhabditis elegans gene product and to antigens of the filarial nematodes but not to other known proteins. In addition, As14 homologues were found to be expressed in human and dog roundworms. In mice that received intranasal administration of rAs14 coupled with cholera toxin B subunit (rAs14-CTB), there was a 64% reduction of recovery of larvae compared with that in the nontreated group. The vaccinated mice showed a significant increase in the total serum immunoglobulin G (IgG) levels and the mucosal IgA responses. Elevation of the rAs14-specific IgE response was also seen. Measurement of the IgG subclasses showed a higher level of IgG1 and a lower level of IgG2a antibody response in the sera of the immunized mice, suggesting that protection was associated with a type II immune response. As14 is the first protective antigen against A. suum infection to be identified. Our immunization trial results in laboratory animals suggest the possibility of developing a mucosal vaccine for parasitic diseases caused by ascarid nematodes.

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Patent infections of Ascaris suum in pigs: Effect of previous exposure to multiple, high doses of eggs and various treatment regimes

Stankiewicz

Jonas

Froe

1992

International Journal for Parasitology

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Responses of NFR/N inbred mice to very low-dose infections with Ascaris suum

Cited by 9 publications

References 15 publications

Mice Intranasally Immunized with a Recombinant 16-Kilodalton Antigen from RoundwormAscarisParasites Are Protected against Larval Migration ofAscaris suum

Mice Intranasally Immunized with a Recombinant 16-Kilodalton Antigen from RoundwormAscarisParasites Are Protected against Larval Migration ofAscaris suum

Intranasal Immunization with RecombinantAscaris suum14-Kilodalton Antigen Coupled with Cholera Toxin B Subunit Induces Protective Immunity toA. suumInfection in Mice

Patent infections of Ascaris suum in pigs: Effect of previous exposure to multiple, high doses of eggs and various treatment regimes

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