Responses of mares to rechallenge with the organism of contagious equine metritis 1977

Timoney, PJ; O'Reilly, PJ; McArdle, JF; Ward, John A.; Harrington, AM

doi:10.1136/vr.104.12.264

Cited by 8 publications

(7 citation statements)

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“…The duration of CEM infection was appreciably shorter than that reported in strains of albino Swiss [8], ICR [9] or CBA/N and LAFffJ mice (P. J. Timoney, S. J. Shin and R. H. Jacobsen, submitted for publication). In contrast to the response of pony mares following reexposure to CEMO [21], there was no significant reduction in the shedding period compared with the corresponding interval after primary challenge. The method and frequency of sampling [24] were not considered to have had any major influence on the pattern of isolations of CEMO.…”

Section: Discussioncontrasting

confidence: 68%

“…Responses of the cats to rechallenge with CEMO can be compared with those reported in mares following experimental reexposure to the organism. Sahu et al [19] and Timoney et al [21] successfully reinfected pony mares using large inocula. Clinical, bacteriological and serological responses were less than those originally observed after primary challenge and evidence was detected of local immunity in the genital tract.…”

Section: Discussionmentioning

confidence: 99%

“…antibody activity in any of the cats after primary or secondary challenge with CEMO, may be reflective of the limited susceptibility of the species to this organism. Various groups of workers have found the complement-fixation test to be insensitive for the demonstration of serological responses in CEM-infected pony mares [21,27,28] and donkey mares (P. J. Timoney, P. J. O'Reilly, J. F. McArdle and J. Ward, submitted for publication).…”

Section: Discussionmentioning

confidence: 99%

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Transmissibility of the contagious equine metritis organism for the cat

Timoney

Shin

Lein

et al. 1984

Comparative Immunology, Microbiology and Infectious Diseases

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A group of SPF cats were moderately susceptible to the causal organism of contagious equine metritis (CEM) following intra-uterine or intrapreputial challenge with an Irish streptomycin resistant strain isolated from a clinically infected mare. Subclinical infections were established in only 50% of the cats, none of which became long-term carriers of the organism. Cytological examination of vaginal smears was of no diagnostic value in confirming infection in inapparently infected cats. Bacteriological responses after primary or secondary challenge with the CEM organism were essentially similar, with one exception, a female cat in which there was possible evidence of local immunity persisting after the primary infection. Efforts to reactivate shedding subsequent to the immediate post-challenge period were unsuccessful. Throughout the experimental period, the cats remained sero-negative to the complement-fixation test, and they failed to develop any significant increase in the levels of antibody activity as measured by the kinetics-based ELISA or KELA system. On day 89 after primary challenge, the cats were euthanized and various sites in the genitourinary tract and the internal iliac lymphatic glands subjected to bacteriological and pathological examination for evidence of CEM infection with negative results. The findings of this study, although establishing the transmissibility of the CEM organism for the cat, demonstrate the limited value of this species as an experimental model system for the disease in the horse.

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Section: Discussioncontrasting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Transmissibility of the contagious equine metritis organism for the cat

Timoney

Shin

Lein

et al. 1984

Comparative Immunology, Microbiology and Infectious Diseases

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“…Clinically, CEM shares some of the features of gonorrhea in humans. Specifically, leukorrhea, asymptomatic infections, chronic infections, and the generation of antibodies which do not prevent reinfection in the immunocompetent host occur in both diseases (6,26,27,44,45). Interest in the mechanisms of pathogenesis and in the appropriateness of CEM as an animal model for gonorrhea prompted studies of the contagious equine metritis organism (CEMO).…”

mentioning

confidence: 99%

Morphology of three strains of contagious equine metritis organism

Hitchcock¹,

Brown²,

Corwin³

et al. 1985

Infect Immun

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Examination of recently isolated cultures of three strains of Contagious Equine Metritis Organism grown on specially formulated, serum-free, clear typing medium revealed the presence of numerous colonial opacity variants. These colonies were prepared by a number of fixation and staining techniques and examined by scanning and transmission electron microscopy. Opaque and transparent phenotypes produced copious amounts of extracellular material compared with intermediate-opacity phenotypes which produced little or none. Also unique to intermediate colonies were numerous thin intercellular strands, which may represent pili or polymers of extracellular material. The presence of an unusual fibrillar layer (with similar electron density to the extracellular material) on the outer leaf of the outer membrane also was confirmed. A number of other ultrastructural features also were noted, including an epilayer, a thin nonmembranous layer which covered colonies and adjacent agar.

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“…After experimental or natural infection, CEM is characterized by a mucopurulent uterine discharge, with an associated vaginitis. A systemic humoral response has been measured after local infection (4), and specific antibody has been detected in uterine secretions (19,23). However, the organism may be isolated from recovered mares for extended periods after initial infection (4), and immunization or previous infection may not protect against subsequent challenge (8,19).…”

mentioning

confidence: 99%

Nonimmune binding of equine immunoglobulin by the causative organism of contagious equine metritis, Taylorella equigenitalis

Widders¹,

Stokes²,

Newby³

et al. 1985

Infect Immun

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This study identifies nonimmune binding of equine immunoglobulin by the causative organism of contagious equine metritis. Immunoglobulin binding to the bacterium was strongest for immunoglobulin G (IgG) and less for IgM; IgA was not bound. Binding of equine IgG was inhibited by human IgG, but not by IgG of domestic animals. Immunoglobulin binding by the bacterium appeared to be directed towards an epitope in the hinge region of the immunoglobulin molecule.

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Responses of mares to rechallenge with the organism of contagious equine metritis 1977

Cited by 8 publications

References 0 publications

Transmissibility of the contagious equine metritis organism for the cat

Transmissibility of the contagious equine metritis organism for the cat

Morphology of three strains of contagious equine metritis organism

Nonimmune binding of equine immunoglobulin by the causative organism of contagious equine metritis, Taylorella equigenitalis

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