Responses of L929 mouse fibroblasts, primary and immortalized bovine dental papilla-derived cell lines to dental resin components

In VitroComparison of the Effects of Dental Filling Materials on Mouse Fibroblasts

“…According to the standards laid down in this protocol, for the investigation of the cytotoxicity of dental materials and the evaluation of fibroblast attachment in in vitro studies, either L929 or Balb-c 3T3 fibroblast cultures should be used (21). In the light of previously conducted studies (48,58) and for the sake of compliance with the ISO protocol, L929 mouse fibroblast cell cultures were used in our experiments.…”

“…Investigations on the cytotoxicity of various restorative materials were expanded to the quantitative analyses of various biological endpoints in different cell types (50,58). Owing to their reproducible growth rates and biological responses, continuous cell lines, such as L929 mouse fibroblasts, are routinely used for the testing of the cytotoxicity of dental materials.…”

“…In addition, these cells are recommended by international standards for the testing of medical devices used in dentistry because of the ease to control cell culture conditions (19,20). Furthermore, it has been shown that cultures of the continous L929 cell line are more sensitive than the primary human gingival fibroblasts (50,58). Despite the wide use of conventional glass ionomer cements, resin-modified glass ionomer cement and compomers for root coverage, only a few studies have compared their cytotoxic effect on L929 mouse fibroblasts.…”

İki Farklı Yumuşak Astar Materyalinin Sitotoksik Özelliklerinin İncelenmesi

“…Devamlı hücre dizilerinden olan 3T3 ve L 929 fare fibroblast hücreleri dental malzemelerin biyouyumluluk çalışmalarında kullanılmak üzere uluslararası standartlar tarafından önerilmekte ve uzun yıllardır kullanılmaktadır (24,25). Thonemann ve ark da L-929 fare fibroblastlarının insan gingival fibroblast hücrelerine göre daha hassas olduğunu bildirmişlerdir (26). Bu nedenlerden dolayı çalışmada kullanılan yumuşak astar materyallerinin sitotoksisitenin değerlendirilmesi için fare fibroblast hücreleri (L929 hücreleri) kullanıldı.…”

Effects of visible light-irradiated camphorquinone and 9-fluorenone on murine oral mucosa

“…At the same time, CQ is also characterized as a potential allergenic compound 2) . However, studies on the adverse effects of CQ in resin systems have focused mainly on photoinitiators [3][4][5][6][7][8] , while studies on VL-irradiated systems have been scarce. The cytotoxicity of aliphatic CQ and its aromatic counterparts -such as 9-fluorenone (9F), benzyl, and benzophenone -upon exposure to VL has been studied previously using a human submandibular gland carcinoma cell line and human gingival fibroblasts 3,5,7,8) .…”