Responses of in vitro cell cultures to elicitation: regulatory role of jasmonic acid and methyl jasmonate: a review

Nabi, Neelofer; Singh, Seema; Saffeullah, Peer

doi:10.1007/s11627-020-10140-6

Cited by 47 publications

(21 citation statements)

References 138 publications

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“…Many authors have described that the addition of biotic and abiotic elicitors caused an increase in the synthesis of secondary metabolites in in vitro cultures. Methyl jasmonate as a PGR is a biotic elicitor [ 23 ]. In plants, methyl jasmonate induces a defence response to injury or pathogen attack by activating a number of enzymes involved in the biosynthesis of polyphenolic metabolites.…”

Section: Resultsmentioning

confidence: 99%

“…Methyl jasmonate, a biotic elicitor, was used in the elicitation experiment. Methyl jasmonate is often used as an elicitor due to the fact that it activates secondary metabolite production in plants cells as a defence response [ 23 ] ( Figure S1 ). In addition, commercially available Plantform bioreactor temporary immersion systems (TIS), which are ideal for microshoot cultures, were used in the preliminary experiment for maintaining the S. lateriflora cultures at a larger biotechnological scale for the production of antioxidants ( Figure S2 ).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Potential and Enhancement of Bioactive Metabolite Production in In Vitro Cultures of Scutellaria lateriflora L. by Biotechnological Methods

et al. 2022

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Studies carried out using three different in vitro assays and a biological setting (Escherichia coil) demonstrated the antioxidant activity of Scutellaria lateriflora microshoot extract. Moreover, the extract exhibited no toxicity in a brine shrimp lethality bioassay. These results indicated that microshoots are a rich, safe source of antioxidants, which encouraged us to enhance their production in vitro. In agar and agitated cultures, two biotechnological strategies were applied: feeding the cultures with the biogenetic precursors of the phenolics—phenylalanine and tyrosine, and eliciting them with methyl jasmonate. Specific Scutellaria flavonoids and verbascoside were analysed by HPLC. Feeding with precursors (1 g/L) in agar cultures decreased the production of the metabolites. In agitated cultures, different concentrations of precursors (1.0–2.5 g/L) and the elicitor (10; 50; 100 µM) were tested. Additionally, parallel feeding with the precursor and elicitor in a concentration of 50 µM were applied. The best strategy for total flavonoid and verbascoside production was phenylalanine feeding (1.5 g/L), max. 3765 and 475 mg/100 g DW, respectively, after 7 days. This is the first report documenting the high antioxidant production in S. lateriflora microshoots after feeding with phenylalanine. Moreover, for the first time, bioreactor cultures were successfully maintained, obtaining attractive results (max. total flavonoid content 2348 and verbascoside 485 mg/100 g DW).

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidant Potential and Enhancement of Bioactive Metabolite Production in In Vitro Cultures of Scutellaria lateriflora L. by Biotechnological Methods

et al. 2022

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“…Among the strategies used for improving the production of PDSM in CSC is the modification in the culture media composition (different carbon, nitrogen, and phosphorous sources) for optimizing the nutrient availability during the culture time [6,9], and the use of biotic or abiotic elicitors that trigger the defense response from plant cells promoting the secondary metabolism through the introduction of chemical or physical stresses [7,51,95]. Biotic elicitors are complex compounds derived from biological sources, including plant-derived polysaccharides, such as pectin and cellulose, and microbial-derived polysaccharides, such as chitin and glucan [6,44], and plant immune-signaling molecules, such as jasmonic acid [96], salicylic acid [97,98], and methyl jasmonate [96]. Abiotic elicitors include inorganic salts, heavy metals, UV irradiation, high salinity, and pressure [99].…”

Section: Plant Cell Suspension Culturementioning

confidence: 99%

Engineering Considerations to Produce Bioactive Compounds from Plant Cell Suspension Culture in Bioreactors

Motolinía-Alcántara

Castillo‐Araiza

Rodrı́guez-Monroy

et al. 2021

Plants

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The large-scale production of plant-derived secondary metabolites (PDSM) in bioreactors to meet the increasing demand for bioactive compounds for the treatment and prevention of degenerative diseases is nowadays considered an engineering challenge due to the large number of operational factors that need to be considered during their design and scale-up. The plant cell suspension culture (CSC) has presented numerous benefits over other technologies, such as the conventional whole-plant extraction, not only for avoiding the overexploitation of plant species, but also for achieving better yields and having excellent scaling-up attributes. The selection of the bioreactor configuration depends on intrinsic cell culture properties and engineering considerations related to the effect of operating conditions on thermodynamics, kinetics, and transport phenomena, which together are essential for accomplishing the large-scale production of PDSM. To this end, this review, firstly, provides a comprehensive appraisement of PDSM, essentially those with demonstrated importance and utilization in pharmaceutical industries. Then, special attention is given to PDSM obtained out of CSC. Finally, engineering aspects related to the bioreactor configuration for CSC stating the effect of the operating conditions on kinetics and transport phenomena and, hence, on the cell viability and production of PDSM are presented accordingly. The engineering analysis of the reviewed bioreactor configurations for CSC will pave the way for future research focused on their scaling up, to produce high value-added PDSM.

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“…Bu tetiklenme, üretilen metabolit miktarının biyotik ya da abiyotik stres sonucu elisitasyon ile artmasını sağlamaktadır [7]. Elisitasyon, in vitro ve ex vivo gelişen bitkilerde biyokütlenin ve sekonder metabolit üretimlerinin artırılması olarak tanımlanmaktadır [73]. Elisitasyonda kullanılan elisitörler, bu işlemi sinyal iletim kaskatlarına müdahale ederek gerçekleştirirler [49].…”

Section: Bitkisel Sekonder Metabolitlerin Kullanımı Ve Covid-19 Ile Mücadeleunclassified

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2021

Ömer Halisdemir Üniversitesi Mühendislik Bilimleri Dergisi

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Responses of in vitro cell cultures to elicitation: regulatory role of jasmonic acid and methyl jasmonate: a review

Cited by 47 publications

References 138 publications

Antioxidant Potential and Enhancement of Bioactive Metabolite Production in In Vitro Cultures of Scutellaria lateriflora L. by Biotechnological Methods

Antioxidant Potential and Enhancement of Bioactive Metabolite Production in In Vitro Cultures of Scutellaria lateriflora L. by Biotechnological Methods

Engineering Considerations to Produce Bioactive Compounds from Plant Cell Suspension Culture in Bioreactors

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