Engineering Considerations to Produce Bioactive Compounds from Plant Cell Suspension Culture in Bioreactors

Abstract

The large-scale production of plant-derived secondary metabolites (PDSM) in bioreactors to meet the increasing demand for bioactive compounds for the treatment and prevention of degenerative diseases is nowadays considered an engineering challenge due to the large number of operational factors that need to be considered during their design and scale-up. The plant cell suspension culture (CSC) has presented numerous benefits over other technologies, such as the conventional whole-plant extraction, not only for avoiding the overexploitation of plant species, but also for achieving better yields and having excellent scaling-up attributes. The selection of the bioreactor configuration depends on intrinsic cell culture properties and engineering considerations related to the effect of operating conditions on thermodynamics, kinetics, and transport phenomena, which together are essential for accomplishing the large-scale production of PDSM. To this end, this review, firstly, provides a comprehensive appraisement of PDSM, essentially those with demonstrated importance and utilization in pharmaceutical industries. Then, special attention is given to PDSM obtained out of CSC. Finally, engineering aspects related to the bioreactor configuration for CSC stating the effect of the operating conditions on kinetics and transport phenomena and, hence, on the cell viability and production of PDSM are presented accordingly. The engineering analysis of the reviewed bioreactor configurations for CSC will pave the way for future research focused on their scaling up, to produce high value-added PDSM.

Establishment of Stem Cell-like Cells of Sida hermaphrodita (L.) Rusby from Explants Containing Cambial Meristems

“…Weight of callus cell biomass derived from stem segments was 11–15 times higher in comparison with leaf, petiole, and root explants after 16 weeks of growth ( Figure 5 , Table S1 ). The establishment of cell suspension cultures from callus cultures typically has a higher potential for the production of cell biomass and secondary metabolites, mainly of pharmaceutical and medicinal interest [ 28 , 55 ]. Similar applications are associated at present with in vitro cultured plant stem cells.…”

Cell‐cultivated food production and processing: A review

“…Compared with microorganism cells, plant cells are also more sensitive to the environment. Motolinía-alcántara et al (2021) suggested that compared with conventional whole-plant extraction, plant cell culture can avoid overexploitation of plant species, achieve better yields, and can be easily scaled up.…”

“…Detailed description and applications of these reactors were reviewed by Furusaki and Takeda (2019) and Su (1995). As with the culturing of other cell types, the selection of a bioreactor for plant cell culture depends on the properties of the cell cultures and engineering considerations related to thermodynamics, kinetics, and transport phenomena (Motolinía-alcántara et al, 2021).…”

Effect of Aspergillus niger extract on production of coumarins in cell suspension cultures of Angelica archangelica

“…Cell suspension cultures are one of the important techniques in the plant cell tissue cultures field due to their biological applications as it is used for the analysis of complex plant physiological processes on a cellular and molecular level, ion transport, enzyme induction, defense responses, and gene expression (Motolinía Alcántara et al 2021). On top of that is the high ability of cell suspensions to perform physiological processes in the liquid nutritional medium to which suitable physiological regulators were added.…”