Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene

Lamichhane, Sujan; Anderson, Jordan A.; Remund, Tyler; Sun, Hongli; Larson, Mark K.; Kelly, Patrick; Mani, Gopinath

doi:10.1002/jbm.a.35763

Cited by 28 publications

(24 citation statements)

References 48 publications

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“…Cell‐surface interactions are tailored by altering surface properties . Here, Ta incorporation and nano‐structure fabrication were simultaneously achieved.…”

Section: Discussionmentioning

confidence: 99%

Bacterial and mammalian cells adhesion to tantalum‐decorated micro‐/nano‐structured titanium

Zhu

Qiao

et al. 2016

J Biomedical Materials Res

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Microorganisms are frequently introduced to dental implants during surgery and start the race for the surface with host cells before osseointegration occurs. The aim of the study was to endow implant surfaces with biological functions that reliably select cells over microbes. Nano-structured tantalum (Ta) has exhibited excellent compatibility. Thus, nano-structured Ta films were deposited on the sand-blasted, large grit, and acid-etched (SLA) titanium by the magnetron sputtering method, thus forming hierarchical micro-/nano-structured surfaces. No obvious Ta release confirmed the robustness of the deposited layer probably arising from the stable Ta O . Moreover, Ta-modified surfaces not only improved the initial adhesion and spreading of rat bone mesenchymal stem cells (rBMSCs), but also exhibited good antibacterial activities towards Streptococcus mutans and Porphyromonas gingivalis. The satisfactory cell-surface interactions on Ta-modified surfaces depended largely on the up-regulation of adhesion-related genes and activation of focal adhesion kinase (FAK), as confirmed by real-time PCR and Western blot. Here, the coculture model was also forwarded to mimic the perioperative bacterial contamination. We found that the adherent cell number and the cell-surface coverage were hampered by bacteria presence on both surfaces. Yet, rBMSCs still attached and spread more readily on Ta-modified surfaces than on SLA titanium surfaces even in coculture with adhering oral pathogens. Our results revealed that Ta-modified micro-/nano-structured surfaces would selectively promote cell-surface rather than bacteria-surface interactions, boding well for the applications for dental implants in possibly infected environments. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 871-878, 2017.

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“…Cell‐surface interactions are tailored by altering surface properties . Here, Ta incorporation and nano‐structure fabrication were simultaneously achieved.…”

Section: Discussionmentioning

confidence: 99%

Bacterial and mammalian cells adhesion to tantalum‐decorated micro‐/nano‐structured titanium

Zhu

Qiao

et al. 2016

J Biomedical Materials Res

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“…Flat PTFE, ePTFE, and electrospun PTFE were prepared as we previously reported . Briefly, the PTFE materials were cut into 1 cm × 1 cm samples, immersed in absolute ethanol for 2 min and dried in a biological safety cabinet for 18 h before the specimens were used for macrophage interaction studies.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, we investigated the interaction of endothelial cells (ECs), smooth muscle cells (SMCs), and platelets with different topographies of PTFE . We performed a thorough surface characterization of three different forms of PTFE: flat PTFE, expanded PTFE (ePTFE), and electrospun PTFE that are currently used for making various cardiovascular devices.…”

Section: Introductionmentioning

confidence: 99%

Polytetrafluoroethylene topographies determine the adhesion, activation, and foreign body giant cell formation of macrophages

Lamichhane

Anderson

Vierhout

et al. 2017

J Biomedical Materials Res

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Polytetrafluoroethylene (PTFE) is one of the commonly used materials in making various cardiovascular implants. However, the success rates of these implants in several occasions are hindered by unwanted immune responses from immune cells, such as macrophages. In this study, we investigated the response of macrophages with different structures (flat, expanded, and electrospun) of PTFE having varied surface topographies: smooth planar surface (flat PTFE), node-fibrils (ePTFE), and randomly oriented microfibers (electrospun PTFE). The electrospun PTFE showed the least adhesion of macrophages. Also, the morphology of macrophages adhered on electrospun PTFE exhibited minimal activation. The macrophage pro-inflammatory cytokine secretions showed that the lowest level of TNF-α was produced on electrospun PTFE whereas IP-10 was produced in lowest levels on expanded PTFE (ePTFE). The production of IL-6 and MCP-1 cytokines was also dependent on the structure of PTFE that the macrophages interacted with, but in a time-dependent manner. Confocal microscopy images taken at 7, 14, and 21 days showed that the electrospun PTFE resulted in the lowest percentage of macrophage fusion, thus indicating the least possible chance of foreign body giant cell (FBGC) formation. Therefore, this study showed that electrospun PTFE with randomly oriented microfibers can provide reduced adhesion, activation, and FBGC formation of macrophages compared to the smooth and planar surface of flat PTFE and node-fibril structured surface of ePTFE. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2441-2450, 2017.

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“…13 The impact of the diameter and orientation of the nano-and micro-fibers on the platelet aggregation, although inconclusive in several publications, can influence the coagulation cascade and platelet adhesion. 30 Despite little clinical and commercial success, nano-fibers have a strong potential for application as hemostatic agents.…”

Section: Clinical In Vitro and In Vivo Modelsmentioning

confidence: 99%

<p>A review of the application of cellulose hemostatic agent on trauma injuries</p>

Khoshmohabat¹,

Paydar²,

Makarem³

et al. 2019

OAEM

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Introduction: Planning for management of bleeding in trauma injuries is very important. The initial purpose in emergency situations should be immediate establishment of an efficient hemostasis, principally in its topical application. In this study, we aimed to review the major relevant articles in the case of application of cellulose hemostatic agent on trauma injuries. Methods: We searched the online databases such as PubMed, MEDLINE, Wiley, EMBASE, ISI Web of Knowledge, and Scopus. Two reviewers independently searched and assessed the titles and abstracts of all articles. Results: Upon screening the titles and abstracts, 24 studies were identified for full-text review. The oxidized cellulose had the best clotting times, while it demonstrated low absorption ability. Surgical and thermosensitive chitosan hemostatic could be valuable for managing hemorrhage from liver injuries in trauma patients. Conclusion: Recently, the application of cellulose hemostatic agents has been one of the main improvements obtained for controlling bleeding in trauma injuries. However, generally according to the literature review, the decision about using each agent should be made on a case-by-case basis. However, it can be mentioned that the perfect hemostatic agent has not been still identified.

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Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene

Cited by 28 publications

References 48 publications

Bacterial and mammalian cells adhesion to tantalum‐decorated micro‐/nano‐structured titanium

Bacterial and mammalian cells adhesion to tantalum‐decorated micro‐/nano‐structured titanium

Polytetrafluoroethylene topographies determine the adhesion, activation, and foreign body giant cell formation of macrophages

<p>A review of the application of cellulose hemostatic agent on trauma injuries</p>

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