Responses of date palm (Phoenix dactylifera L.) callus to biotic and abiotic stresses

Abass, Mohammed H.

doi:10.9755/ejfa.2015-09-799

Cited by 10 publications

(8 citation statements)

References 37 publications

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“…Electrophoresis was conducted in a 1X TBE buffer [100 mL 10X TBE (0.89 M Tris base, 0.89 M Boric acid, 20 mM EDTA pH 8.0) and 900 mL distilled water] at 60 V for 30 min and then at 120 V for 1.5 h. The gDNA was stained with 0.5 mg/mL of ethidium bromide. gDNA concentration was measured by Nano-Drop spectrophotometer (Bio-Rad, USA) at A260/280, gDNA templates were diluted to 30 ng/μl with TE buffer [3].…”

Section: Methodsmentioning

confidence: 99%

“…Date palm ( Phoenix dactylifera L.) is one of the most important members of the family Arecaceae, a dioecious, perennial, monocotyledonous tree cultivated mainly for its nutritive fruits as well as an ornamental plant [3]. Date palm tree plays a pivotal role in the sustainable agriculture in many countries around the world with a long history of cultivation and utilization in Middle East and north Africa for more than five thousand years [10].…”

Section: Introductionmentioning

confidence: 99%

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Genotoxicity assessment of high concentrations of 2,4-D, NAA and Dicamba on date palm callus ( Phoenix dactylifera L.) using protein profile and RAPD markers

Abass

Al-Utbi

Al-Samir

2017

Journal of Genetic Engineering and Biotechnology

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Genetic stability and uniformity of in vitro-derived date palm plants has a major importance to ascertain true-to-typeness of produced plants. The goal of present study was to evaluate the genetic toxicity of different plant growth regulators on date palm callus at initiation stages using protein patterns and RAPD analysis. Date palm offshoots of Hillawii cultivar were dissected, apical meristems were divided into four segments and cultured on callus induction medium containing the plant growth regulators as 2,4-D at 50 and 100 mg/L; NAA at 30 mg/L and Dicamba at 10 mg/L. The changes occurred in protein profile of callus when treated with high concentration of 2,4-D (100 mg/L), including loss of normal fragments (19 and 66 KDa polypeptides in control), as well as, appearance of new fragments, while at low concentration of 2,4-D (50 mg/L) and Dicamba treatment, the protein patterns showed no changes compared to control profile. Similar trends of polymorphisms were obtained with RAPD marker. The high concentration of 2,4-D produced more polymorphic fragments in comparison to control treatment. The DNA profile was identical between 2,4-D at low concentration and control. Dendrograms were generated using similarity indices of protein and RAPD results, and revealed that genetic similarity index was high between 2,4-D treatment at low concentration and control, as separated in one subcluster, followed by Dicamba and NAA, while, the highest genetic distance was obtained between 2,4-D at high concentration and control treatment and separated alone in one cluster.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genotoxicity assessment of high concentrations of 2,4-D, NAA and Dicamba on date palm callus ( Phoenix dactylifera L.) using protein profile and RAPD markers

Abass

Al-Utbi

Al-Samir

2017

Journal of Genetic Engineering and Biotechnology

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“…Browning is induced by oxidation on a plant tissue surface. The oxidation of phenolic compounds produces quinones that are highly reactive and toxic to cultured tissues (Zaid, 1984, Abohatem et al, 2011, Al-Samir et al, 2015, Abass, 2016. The browning of cultured date palm tissues is probably one of the causes of growth retardation.…”

Section: Phytotoxic Effect Of Decontamination Agents On Date Palm Promentioning

confidence: 99%

“…In micropropagation, selected superior cultivars can be rapidly propagated for commercial and environmental interests. This method can be considered as an acceptable approach to satisfy the world's demand of approximately 1-2 million superior date palm cultivars per year (Jain, 2007, Abass, 2016. Date palm micropropagation can be applied in two different procedures, namely, organogenesis and somatic embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

Morphological and biochemical impact of different decontamination agents on date palm (Phoenix dactylifera L.) procallus

Abass¹,

Al-Utbi²,

Al-Samir³

2016

Aust J Crop Sci

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This study was conducted to evaluate the phytotoxic effects of different antibiotics and fungicides on the procallus of date palm (Phoenix dactylifera L.). The fungicides Switch® and Beltanol and the antibiotics chloramphenicol and gentamycin were used in this study. Results confirmed the lethal effects of Switch®, gentamycin, and chloramphenicol (at 100 mg/l in dicamba treatment) on date palm tissues. The treated shoot tips did not develop into procallus, and their growth was completely inhibited. Beltanol treatments significantly decreased the biochemical parameters of the procallus treated with 2, 4-D and dicamba. In the Beltanol-treated tissues, the initial period of callus induction was prolonged from 63.33 and 69.66 days to 97.66 and 117.66 days. The callus induction percentage of the plants treated with the decontamination agents was also decreased. The fresh and dry weights of the procallus and the browning percentage and intensity of the cultured tissues in Beltanol and chloramphenicol treatments (100 mg/l) increased compared with those in the control treatments. Total carbohydrates, free proline content, total phenolic compounds, free amino acid, total soluble protein, and peroxidase activity were significantly affected by Beltanol and chloramphenicol (100 mg/l). By contrast, low chloramphenicol concentrations resulted in acceptable levels of biochemical components. Therefore, we recommend the use of low chloramphenicol and Beltanol concentrations instead of Switch and gentamycin as decontamination agents to prevent or treat microbial contamination in date palm tissue cultures.

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“…ZnO is non-toxic, stable under high temperature, easy to prepare, and costeffective (Hassan et al, 2013;Mirzaeia and Darroudi, 2017;Asuncion et al, 2018).The positive effect of ZnO NPs on the growth of plants and their development has been demonstrated in different plants, including peanut (Arachis hypogaea), soybean (Glycine max), wheat (Triticum aestivum), onion (Allium cepa), banana (Musa paradisiaca) and sesame (Sesamum indicum) (Prasad et al, 2012;Ramesh et al, 2014;Raskar and Laware, 2014;Helaly et al, 2014;Narendhran et al, 2017). Plant tissue culture technique is designed to maintain and multiply the growth of plant cells or different parts on specific cultural medium supplemented with different nutrients and hormones taking the advantage of totipotency (Abass, 2016a). This technique has been employed for various applications, including large-scale production, germplasm conservation, pathogen-free plant production, genetic transformation, genetic toxicity, metabolite production, embryogenesis, morphogenesis, and nutrition (Thorpe, 2007;Abass et al, 2017;Kim et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

In vitro Assessment of ZnO Nanoparticles on Phoenix dactylifera L. Micropropagation

Awad¹

2020

SJKFU

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Microbial contaminants are the major challenges in plant's in vitro propagation during different stages of culture processes, ZnO-NPs exhibit attractive antibacterial properties due to increased specific surface area as the reduced particle size leading to enhanced particle surface reactivity. The aim of present study was to evaluate the effect of ZnO NPs on microbial contamination; vitrification and growth promoting of date palm cultured tissues. Several concentrations of ZnO NPs were selected to assess their activity. The percentage of shoot formation, the number of proliferated shoot and the percentage of contamination of cultures were investigated, as well as several biochemical characteristics. Twofold of increase multiplication rate of proliferated shoot was observed in ZnO NPs at 150 mg L −1 compared to control treatment. The multiplication rate was 46.6% at control and increased significantly to 86.67% and 93.34% in ZnO NPs before and after sterilisation, respectively. No microbial contamination and vitrification were observed at all ZnO NP treatments compared with control treatment. Biochemical analysis showed that ZnO NPs had no toxic effects at all examined concentrations on date palm cultured tissues. A positive effect was observed in carbohydrates, protein and amino acid accumulations at high ZnO concentration (150 mg l −1). The results provide basis for the application of ZnO NPs in media date palm tissue cultures at 150 mg l −1 .

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Responses of date palm (Phoenix dactylifera L.) callus to biotic and abiotic stresses

Cited by 10 publications

References 37 publications

Genotoxicity assessment of high concentrations of 2,4-D, NAA and Dicamba on date palm callus ( Phoenix dactylifera L.) using protein profile and RAPD markers

Genotoxicity assessment of high concentrations of 2,4-D, NAA and Dicamba on date palm callus ( Phoenix dactylifera L.) using protein profile and RAPD markers

Morphological and biochemical impact of different decontamination agents on date palm (Phoenix dactylifera L.) procallus

In vitro Assessment of ZnO Nanoparticles on Phoenix dactylifera L. Micropropagation

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