Genetic stability and uniformity of in vitro-derived date palm plants has a major importance to ascertain true-to-typeness of produced plants. The goal of present study was to evaluate the genetic toxicity of different plant growth regulators on date palm callus at initiation stages using protein patterns and RAPD analysis. Date palm offshoots of Hillawii cultivar were dissected, apical meristems were divided into four segments and cultured on callus induction medium containing the plant growth regulators as 2,4-D at 50 and 100 mg/L; NAA at 30 mg/L and Dicamba at 10 mg/L. The changes occurred in protein profile of callus when treated with high concentration of 2,4-D (100 mg/L), including loss of normal fragments (19 and 66 KDa polypeptides in control), as well as, appearance of new fragments, while at low concentration of 2,4-D (50 mg/L) and Dicamba treatment, the protein patterns showed no changes compared to control profile. Similar trends of polymorphisms were obtained with RAPD marker. The high concentration of 2,4-D produced more polymorphic fragments in comparison to control treatment. The DNA profile was identical between 2,4-D at low concentration and control. Dendrograms were generated using similarity indices of protein and RAPD results, and revealed that genetic similarity index was high between 2,4-D treatment at low concentration and control, as separated in one subcluster, followed by Dicamba and NAA, while, the highest genetic distance was obtained between 2,4-D at high concentration and control treatment and separated alone in one cluster.
This study was conducted to evaluate the phytotoxic effects of different antibiotics and fungicides on the procallus of date palm (Phoenix dactylifera L.). The fungicides Switch® and Beltanol and the antibiotics chloramphenicol and gentamycin were used in this study. Results confirmed the lethal effects of Switch®, gentamycin, and chloramphenicol (at 100 mg/l in dicamba treatment) on date palm tissues. The treated shoot tips did not develop into procallus, and their growth was completely inhibited. Beltanol treatments significantly decreased the biochemical parameters of the procallus treated with 2, 4-D and dicamba. In the Beltanol-treated tissues, the initial period of callus induction was prolonged from 63.33 and 69.66 days to 97.66 and 117.66 days. The callus induction percentage of the plants treated with the decontamination agents was also decreased. The fresh and dry weights of the procallus and the browning percentage and intensity of the cultured tissues in Beltanol and chloramphenicol treatments (100 mg/l) increased compared with those in the control treatments. Total carbohydrates, free proline content, total phenolic compounds, free amino acid, total soluble protein, and peroxidase activity were significantly affected by Beltanol and chloramphenicol (100 mg/l). By contrast, low chloramphenicol concentrations resulted in acceptable levels of biochemical components. Therefore, we recommend the use of low chloramphenicol and Beltanol concentrations instead of Switch and gentamycin as decontamination agents to prevent or treat microbial contamination in date palm tissue cultures.
The shoots of garlic (Allium sativum L.) were initiated on MS (Murashige and Skoog) medium supplemented with Benzyl adenine (BA), 4mg/L which is significantly the best among other treatments (1,2,3,5mg/L); the shoots also initiated from shoot tip in the presence of combination (NAA, 3mg/L and kinetin, 1mg/L); callus was also initiated from basal disc and shoot tip in the presence of combination (NAA, 5mg/L and kinetin 0.5mg/L) and (2,4-D, 1.5mg/L and kinetin 0.5mg/L).R fs values of extracted allicin from bulbs, shoots, and callus were 0.97, 0.94, 0.95 respectively and the amount percentages were 1.3%, 1.8%, 2.2% respectively; identification of allicin by IR spectrum showed the similarity between the groups of extracted allicin from shoots and those of extracted allicin from bulbs (Standard); identification of allicin by UV showed one band at wave length 338nm for extracted allicin from shoot and 296nm for extracted allicin from bulbs (Standard).The results also showed the difference in the biological activity of extracted allicin from callus, shoots and bulbs of garlic toward Staphyllococcus aureus and Escherichia coli.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.