Responses of Calf Kidney Cells to Human Adenovirus Type 12

Abstract

The process of infection of established calf kidney (CKT) cells with human adenovirus type 12 (Ad 12) was compared with that with type 5 (Ad 5). Ad 5 replicated in CKT cells but Ad 12 did not. In CKT cells infected with Ad 5, as much virion antigen was detected by complement-fixation (CF) test as from HeLa cells, and the rate of DNA synthesis increased to about 7 times that in uninfected cells. In CKT cells infected with Ad 12, production of T-antigen was detected by CF test, but neither production of virion antigen nor increase in DNA synthesis was observed. Confluent monolayers of CKT cells incorporated 3H-thymidine progressively into DNA after growth medium was replaced with maintenance medium. This increase in host cell DNA synthesis after changing media was inhibited by infection with Ad 12 or with ultraviolet-irradiated Ad 12.