Establishment of the AU-bek cell line and comparison with two other bovine cell lines

Rossi, C. R.; Kiesel, G. K.

doi:10.1007/bf02618431

Cited by 5 publications

(2 citation statements)

References 21 publications

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“…Virus stocks were produced by infecting cultures of BS/BEK cells (Ferrari et al, 1991), originally obtained from the Istituto Zooprofilattico Sperimentale (Brescia, Italy), propagated in complete medium (CM) consisting of Eagle's MEM (HyClone Europe, Cramlington, UK) containing 10% fetal calf serum (FCS; Life Technologies, Gaithersburg, MD), 5 mM Lglutamin, 100 U/ml penicillin, and 100 g/ml streptomycin, at 37°C in a CO 2 incubator. This cell line, as well as the AU-BEK (Rossi and Kiesel, 1973) and the Madin-Darby bovine kidney (MDBK, originally obtained from ATCC, Rockville, MD) cell lines that were used in some experiments, were all characterized by productive infection by BHV-4, and were found to be free of mycoplasma and bovine viral diarrhea virus. After 72 h of infection, cell extracts, obtained by three cycles of freezing and thawing, were pooled, collected, and stored in aliquots at Ϫ80°C.…”

Section: Viruses Cells and Treatmentsmentioning

confidence: 99%

The Gamma-2-Herpesvirus Bovine Herpesvirus 4 Causes Apoptotic Infection in Permissive Cell Lines

Sciortino¹,

Perri²,

Medici³

et al. 2000

Virology

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Increasing evidence suggests that regulation of apoptosis in infected cells is associated with several viral infections. The gammaherpesvirus bovine herpesvirus 4 (BHV-4) has been shown to harbor genes with antiapoptotic potentialities. However, here we have demonstrated that productive infection of adherent, permissive cell lines by BHV-4 resulted in a cytopathic effect characterized by induction of apoptosis. This phenomenon was confirmed using different techniques to detect apoptosis and using different virus strains and cell targets. Apoptosis induced by BHV-4 was inhibited by (1) treatment with doses of heparin, which completely inhibited virus attachment and infectivity; (2) UV treatment, which completely abrogated infectivity; and (3) treatment with a dose of phosphonoacetic acid, which blocked virus replication. Virus-induced apoptosis was associated with a down-regulation of Bcl-2 expression and was reduced by Z-VAD-FMK, but not by Z-DEVD-FMK (caspase-3-specific) caspase inhibitors. Inhibition of apoptosis by Z-VAD-FMK treatment during infection did not modify virus yield. Therefore, despite the presence of antiapoptotic genes in its genoma, BHV-4 could complete its cycle of productive infection while inducing apoptosis of infected cells. This finding might have implications for the pathobiology of BHV-4 and other gammaherpesviruses in vivo.

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Section: Viruses Cells and Treatmentsmentioning

confidence: 99%

The Gamma-2-Herpesvirus Bovine Herpesvirus 4 Causes Apoptotic Infection in Permissive Cell Lines

Sciortino¹,

Perri²,

Medici³

et al. 2000

Virology

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“…The AU-BEK cell line, derived from bovine foetal kidney (Rossi & Kiesel, 1973), the tissue culture-adapted Lincoln strain of Nebraska calf diarrhoea rotavirus (Mebus et al, 1971) and the Indiana serotype of vesicular stomatitis virus (VSV) were employed throughout the experiments.…”

Section: Cells and Virusesmentioning

confidence: 99%

Rotavirus Persistence in Cell Cultures: Selection of Resistant Cells in the Presence of Foetal Calf Serum

Chiarini

Arista

Giammanco

et al. 1983

Journal of General Virology

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SUMMARYThe Lincoln strain of bovine rotavirus, cytocidal for bovine AU-BEK cells, can establish in the same cell cultures in the presence of foetal calf serum (FCS) a persistent infection that depends on selection of highly resistant cells. In fact, after the induction of the carrier state only a small fraction of the cell population was infected. The parental and the carried viruses are not demonstrably different, the cultures are resistant to superinfection by the homologous virus, the cultures can be cured by antiviral serum in the medium and uninfected resistant cell clones can be selected. The presence of FCS was essential during induction and maintenance of persistence. Its effects appear to be exerted not on the control of virus replication in fully sensitive cells but on the proliferation and selection of the resistant cells.

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Susceptibility of cell line cultures of bovine kidney origin to infectious bovine rhinotracheitis and parainfluenza-3 viruses☆

Ferrari

Gualandi

Castrucci

1981

Comparative Immunology, Microbiology and Infectious Diseases

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Establishment of the AU-bek cell line and comparison with two other bovine cell lines

Cited by 5 publications

References 21 publications

The Gamma-2-Herpesvirus Bovine Herpesvirus 4 Causes Apoptotic Infection in Permissive Cell Lines

The Gamma-2-Herpesvirus Bovine Herpesvirus 4 Causes Apoptotic Infection in Permissive Cell Lines

Rotavirus Persistence in Cell Cultures: Selection of Resistant Cells in the Presence of Foetal Calf Serum

Susceptibility of cell line cultures of bovine kidney origin to infectious bovine rhinotracheitis and parainfluenza-3 viruses☆

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