Response to Fusarium culmorum inoculation in barley

Fusarium culmorum Tri genes and barley Hvugt13248 gene transcription in infected barley cultivars

“…Determination of cv. Amulet as a resistant cultivar is consistent with the evaluation by Vančo et al (2007). Comparing our results with the declared resistance of cultivars (Chrpová et al 2011) shows no consistency.…”

Building on a foundation: advances in epidemiology, resistance breeding, and forecasting research for reducing the impact of fusarium head blight in wheat and barley

“…Following epidemics in the mid 1990s, revitalized screening efforts were conducted in North America, including in the USA (Prom et al 1997;Scholz et al 1999;Steffenson and Scholz 2001;Skoglund and Menert 2002), Canada (McCallum et al 2004;Tucker et al 2009), and Mexico (Gilchrist 2001;Gilchrist et al 2001). Evaluation of germplasm has also been conducted in Europe (Buerstmayr et al 2004;Vančo et al 2007). Many of the accessions identified earlier in eastern Asia have been validated in the North American growing environment, but poor-malting qualities (Urrea et al 2005) and unadapted natures due to photoperiod sensitivity have posed some problems in their utility for breeding (Franckowiak 2001).…”

The effect of Fusarium culmorum inoculation and deoxynivalenol application on proteome response in wheat cultivars Sumai 3 and SW Kadrilj

“…Enhanced content of LOX (ssp 6506) in Sumai with respect to Kadrilj plants under control conditions may indicate an enhanced pathway leading to JA biosynthesis. Besides, LOX produced hydroperoxides of unsaturated fatty acids lead to production of ROS which reveal damage to plant membrane but can also have a signalling function; a close relationship was proposed for LOX and ROS (Siedow 1991, Glickman and Klinman 1996, Maalekuu et al 2006. Thus, enhanced LOX (ssp 6506) in Sumai vs. Kadrilj plants may indicate enhanced LOX-induced and ROS-madiated signalling in Sumai.…”

“…Seeds were sown on 4 th April, 2016 in a greenhouse located at Crop Research Institute, Prague, Czech Republic (50°5′ N; 14°25′ E) under regulated watering till flowering stage. During flowering stage (9 th June 2016; full flowering stage BBCH 65), plants of both cultivars were divided into three groups: control plants, plants inoculated with an inoculum of Fusarium culmorum strain B grown on potato dextrose agar (Sigma-Aldrich, St. Louis, USA) and plants treated with a solution of deoxynivalenol [DON; Sigma-Aldrich, 10 μg mm -3 diluted in phosphate buffer saline (PBS), pH 7.5, 0.05 % Tween-20; 10 mm 3 of DON solution was applied per one spike] using a single-floret method (Vančo et al 2007;Chrpová et al 2011). The spikes of all three plant groups were harvested for proteomic analysis at 10 dai and 10 d after DON application, respectively, when the plants were at early-dough stage (BBCH 73).…”