Response to dietary phosphorus deficiency is affected by genetic background in growing pigs1

Alexander, Lindsey S.; Qu, Ani; Cutler, Sara A.; Mahajan, Avanika; Lonergan, Steven M.; Rothschild, Max F.; Weber, Thomas; Kerr, B. J.; Stahl, Chad H.

doi:10.2527/jas.2007-0692

Cited by 25 publications

(25 citation statements)

References 27 publications

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“…Few studies have been done to elucidate the effects of dietary P levels on gene expression (Hittmeier et al, 2006;Alexander et al, 2008). The results obtained in our study revealed that different aP levels in the diet altered the expression of the electron transport chain genes.…”

Section: Discussionmentioning

confidence: 51%

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Effect of heat stress and feeding phosphorus levels on pig electron transport chain gene expression

Weller

Alebrante

Campos

et al. 2013

Animal

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The purpose of this study was to evaluate the effect of temperature and different levels of available phosphorus (aP) on the expression of nine genes encoding electron transport chain proteins in the Longissimus dorsi (LD) muscle of pigs. Two trials were carried out using 48 high-lean growth pigs from two different growth phases: from 15 to 30 kg (phase 1) and from 30 to 60 kg (phase 2). Pigs from growth phase 1 were fed with three different levels of dietary aP (0.107%, 0.321% or 0.535%) and submitted either to a thermoneutral (24°C and RH at 76%) or to a heat stress (34°C and RH at 70%) environment. Pigs from growth phase 2 were fed with three different levels of dietary aP (0.116%, 0.306% or 0.496%) and submitted either to a thermoneutral (22ºC and RH at 77%) or to a heat stress (32ºC and RH at 73%) environment. Heat stress decreased (P < 0.001) average daily feed i ntake at both growth phases. At 24°C, pigs in phase 1 fed the 0.321% aP diet had greater average daily gain and feed conversion (P < 0.05) than those fed the 0.107% or 0.535% while, at 34°C pigs fed the 0.535% aP had the best performance (P < 0.05). Pigs from phase 2 fed the 0.306% aP had best performance in both thermal environments. Gene expression profile was analyzed by quantitative real-time polymerase chain reaction. Irrespective of growing phase, the expression of six genes was lower (P < 0.05) at high temperature than at thermoneutrality. The lower expression of these genes under high temperatures evidences the effects of heat stress by decreasing oxidative metabolism, through adaptive physiological mechanisms in order to reduce heat production. In pigs from phase 1, six genes were differentially expressed across aP levels (P < 0.05) in the thermoneutral and one gene in the heat stress. In pigs from phase 2, two genes were differentially expressed across aP levels (P < 0.05) in both thermal environments. These data revealed strong evidence that phosphorus and thermal environments are key factors to regulate oxidative phosphorylation with direct implications on animal performance.Keywords: nutrigenomics, oxidative phosphorylation, pig production, heat stress and animal production, phosphorous levels for pig nutrition ImplicationsData from this study are the first to reveal the effects of different available phosphorus (P) levels in Longissimus dorsi gene expression related to oxidative phosphorylation and its implications on pig performance. Moreover, this study showed the effects of high temperatures on the expression of these genes and how this may alter the physiological response to P. This information will contribute to better understand the role of P in energy metabolism and will bring new insights into the comprehension of the effects of high temperatures on nutritional requirements and mitochondrial function.

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Section: Discussionmentioning

confidence: 51%

“…However, little has been published on the analyses of gene expression when studying dietary levels of available phosphorus (aP) for pigs. Only two studies have shown that the need of aP is genotype specific (Hittmeier et al, 2006;Alexander et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Effect of heat stress and feeding phosphorus levels on pig electron transport chain gene expression

Weller

Alebrante

Campos

et al. 2013

Animal

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“…level of renal absorption of this nutrient. Confirming this proposition, Alexander et al (2008), when evaluating aP levels in diets for gilts from two genotypes from 8 to 80 kg, found that plasma levels of hormones associated with organic phosphorus homeostasis maintenance differed not only by phosphorus levels in their diets, but also between the genotypes evaluated. Levels of aP influenced (P<0.01) feed conversion (Table 2), which improved in a quadratic way up to a maximum response of 0.461% (Figure 3).…”

Section: Resultsmentioning

confidence: 64%

“…Frederick and Stahly (1998) studied dietary levels of aP for piglets from 7 to 23 kg and observed that the DFD decreased by increasing aP levels in their diets. Working with pigs during the growing and finishing phases, O'Quinn et al (1997) and Alexander et al (2008) reported that dietary aP deficiency resulted in higher fat thickness measured at the P2 position (10 th rib). The aP deficiencies in the referred studies corresponded to 75 and 80% of the aP levels suggested by the NRC (1998) for both growing and finishing phases.…”

Section: Resultsmentioning

confidence: 99%

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Available phosphorus levels in diets for pigs with high genetic potential for lean meat deposition kept in thermoneutral environment from 15 to 30 kg

Alebrante¹,

Donzele

Oliveira

et al. 2011

R. Bras. Zootec.

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-It was used 72 pigs, 36 castrated males and 36 females, with initial weight of 14.97 ± 0.36 kg to evaluate levels of dietary available phosphorus (aP). The animals were distributed in a completely randomized block design, with six levels of aP (0.107; 0.214; 0.321; 0.428; 0.535 and 0.642%), six replicates and two animals (one castrated male and one female) per experimental unit. Air temperature and relative humidity in the room were kept at 24.5 ± 1.2°C and 76.3 ± 8.5%, respectively. The levels of aP influenced both daily feed intake and daily weight gain, which increased in a quadratic way up to the estimated levels of 0.420% and 0.443%, respectively and feed conversion, which improved in a quadratic manner up to the estimated level of 0.461%. It was observed the effects of levels of aP on quantity of phosphorus and ash in the bone, which increased in a quadratic manner up to the estimated levels of 0.525% and 0.520%, respectively. Levels of Ap affected daily protein deposition in the carcass, which increased in a quadratic way up to the estimated level of 0.394%. There was no effect of levels of aP on daily fat carcass deposition. Levels of aP that provided the best results for daily weight gain, feed conversion and bone mineralization in swines with high genetic potential for lean meat kept in thermoneutral environment, from 15 to 30 kg are 0.443, 0.461 and 0.525%, respectively, corresponding to estimated daily intakes of 5.25, 5.45 and 6.14 g of aP.Key Words: genotype, minerals, requirement, swine, temperature Níveis de fósforo disponível em rações para suínos de alto potencial genético para deposição de carne magra mantidos em ambiente termoneutro dos 15 aos 30 kg RESUMO -Foram utilizados 72 leitões, 36 machos castrados e 36 fêmeas, com peso inicial de 14,97 ± 0,36 kg para avaliar níveis de fósforo disponível (Pd). Os animais foram distribuídos em delineamento experimental de blocos ao acaso, com seis níveis de Pd (0,107; 0,214; 0,321; 0,428; 0,535 e 0,642%), seis repetições e dois animais (um macho castrado e uma fêmea) por unidade experimental. A temperatura interna da sala foi mantida em 24,5 ± 1,2ºC e a umidade relativa do ar em 76,3 ± 8,5%. Os níveis de Pd influenciaram o consumo de ração diário e o ganho de peso diário, que aumentaram de forma quadrática até os níveis estimados de 0,420 e 0,443%, respectivamente, e a conversão alimentar, que melhorou de forma quadrática até o nível estimado de 0,461%. Verificou-se efeito dos níveis de Pd sobre a quantidade de fósforo e cinzas no osso, que aumentou de forma quadrática até os níveis estimados, respectivos, de 0,525 e 0,520%. Os níveis de Pd melhoraram a deposição diária de proteína na carcaça, que aumentou de forma quadrática até o nível estimado de 0,394%, e não afetaram a deposição diária de gordura na carcaça. Os níveis de Pd que proporcionam os melhores resultados de ganho de peso, conversão alimentar e mineralização óssea de suínos de alto potencial genético para deposição de carne magra mantidos em ambiente termoneutro na fase dos 15 aos...

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Lower dietary phosphorus supply in pigs match both animal welfare aspects and resource efficiency

Oster

Gerlinger

Heide

et al. 2017

Ambio

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Dietary phosphorus frequently exceeds age-specific requirements and pig manure often contains high phosphorus load which causes environmental burden at regional scales. Therefore, feeding strategies towards improved phosphorus efficiency and reduced environmental phosphorus load have to be developed. A 5-week feeding trial was conducted: piglets received medium, lower (−25%), or higher (+25%) amounts of phosphorus and calcium. Dietary responses were reflected by performance parameters, bone characteristics, and molecular data retrieved from serum, intestinal mucosa, and kidney cortex (p < 0.05). Transcripts associated with vitamin D hydroxylation (Cyp24A1, Cyp27A1, Cyp27B1) were regulated by diet at local tissue sites. Low-fed animals showed attempts to maintain mineral homoeostasis via intrinsic mechanisms, whereas the high-fed animals adapted at the expense of growth and development. Results suggest that a diet containing low phosphorus and calcium levels might be useful to improve resource efficiency and to reduce phosphorus losses along the agricultural value chain.Electronic supplementary materialThe online version of this article (10.1007/s13280-017-0969-8) contains supplementary material, which is available to authorized users.

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Response to dietary phosphorus deficiency is affected by genetic background in growing pigs1

Cited by 25 publications

References 27 publications

Effect of heat stress and feeding phosphorus levels on pig electron transport chain gene expression

Effect of heat stress and feeding phosphorus levels on pig electron transport chain gene expression

Available phosphorus levels in diets for pigs with high genetic potential for lean meat deposition kept in thermoneutral environment from 15 to 30 kg

Lower dietary phosphorus supply in pigs match both animal welfare aspects and resource efficiency

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