Response to: cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection - authors' reply

Rutgers, Marijn; Saris, Daniël B.F.; Dhert, Wouter J.A.; Creemers, Laura B.

doi:10.1186/ar3192

Cited by 5 publications

(3 citation statements)

References 8 publications

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“…Similar results have been reported in human APS (20). However, Hraha et al described increased concentrations of IL-1β or TNF-α in preparations of equine ACS compared to serum (19) and Rutgers et al found similar results in human ACS (28).…”

Section: Discussionmentioning

confidence: 93%

The Effect of Autologous Protein Solution on the Inflammatory Cascade in Stimulated Equine Chondrocytes

et al. 2019

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Cartilage injury occurs commonly in equine athletes, often precipitating posttraumatic osteoarthritis (PTOA). Orthobiologics such as autologous conditioned serum (ACS) and autologous protein solution (APS) may be useful in decreasing posttraumatic inflammation, thereby preventing PTOA. The objective of this study was to quantify cytokine concentrations in ACS and APS and evaluate the protective effects of ACS and APS on inflamed chondrocytes cultured in vitro. We hypothesized that the combination of platelet-derived growth factors (PDGF) and anti-inflammatory cytokines present in APS would be superior in decreasing the inflammatory and catabolic cascade in inflamed chondrocytes when compared to ACS in which platelets are excluded from the preparation. Chondrocytes were isolated from the cartilage of femoral trochlear ridges of 6 horses and cultured in 12-well transwell plates. Treatment groups included: (1) control, (2) APS (Pro-Stride; Owl Manor), and (3) ACS (IRAP II; Arthrex). Each group was unstimulated or stimulated with IL-1β and TNF-α for 48 h. The concentration of IL-1β, IL-6, TNF-α, MMP-3, MMP-13, and IL-10 was quantified using a fluorescent bead-based multiplex assay. IL-1Ra concentration was quantified using ELISA. APS and ACS both had significantly increased concentrations of IL-1Ra without a concurrent increase in IL-1β concentration. After 48 h of culture, media from chondrocytes treated with APS contained significantly increased concentrations of IL-1Ra and IL-10. APS-treated cultures had increased concentrations of IL-6. Overall, APS effectively concentrated IL-1Ra without an incubation period and media from APS-treated chondrocytes had increased concentrations of chondroprotective (IL-1Ra and IL-10) and modulatory (IL-6) cytokines, which may be beneficial in the treatment of inflammatory conditions such as PTOA.

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Section: Discussionmentioning

confidence: 93%

The Effect of Autologous Protein Solution on the Inflammatory Cascade in Stimulated Equine Chondrocytes

et al. 2019

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“…The first attempts of cocultures provided insightful indications of OA-related degeneration processes. These experiments gave evidence about the release of many inflammatory cytokines not only from the synovial membrane but also from the articular cartilage, by feeding a vicious inflammatory circuit [82][83][84]. For all these aspects, we can consider coculture systems a more complex model than monolayer cultures to recapitulate the inflammatory network.…”

Section: D and Coculture Systems In Cartilage And Synoviummentioning

confidence: 99%

“…Moreover, they showed a drop in T-cell growth in cocultures of chondrocytes with allogeneic T lymphocytes driven by nitric oxide [85]. In general, these systems provided insightful evidence of the impact of synoviocytes on cartilage features and function under inflammatory stimuli [84]. Despite the biological value and insightful perspective from 2D models, they display several drawbacks since they do not mirror the complexity of the OA microenvironment and 3D architecture by limiting mechanistic insights (Table 1).…”

Section: D and Coculture Systems In Cartilage And Synoviummentioning

confidence: 99%

A Roadmap of In Vitro Models in Osteoarthritis: A Focus on Their Biological Relevance in Regenerative Medicine

Bartolotti

Roseti

Petretta

et al. 2021

JCM

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Osteoarthritis (OA) is a multifaceted musculoskeletal disorder, with a high prevalence worldwide. Articular cartilage and synovial membrane are among the main biological targets in the OA microenvironment. Gaining more knowledge on the accuracy of preclinical in vitro OA models could open innovative avenues in regenerative medicine to bridge major gaps, especially in translation from animals to humans. Our methodological approach entailed searches on Scopus, the Web of Science Core Collection, and EMBASE databases to select the most relevant preclinical in vitro models for studying OA. Predicting the biological response of regenerative strategies requires developing relevant preclinical models able to mimic the OA milieu influencing tissue responses and organ complexity. In this light, standard 2D culture models lack critical properties beyond cell biology, while animal models suffer from several limitations due to species differences. In the literature, most of the in vitro models only recapitulate a tissue compartment, by providing fragmented results. Biotechnological advances may enable scientists to generate new in vitro models that combine easy manipulation and organ complexity. Here, we review the state-of-the-art of preclinical in vitro models in OA and outline how the different preclinical systems (inflammatory/biomechanical/microfluidic models) may be valid tools in regenerative medicine, describing their pros and cons. We then discuss the prospects of specific and combinatorial models to predict biological responses following regenerative approaches focusing on mesenchymal stromal cells (MSCs)-based therapies to reduce animal testing.

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Pro- and Anti-inflammatory Cytokine Profiles in Osteoarthritis

Bastiaansen-Jenniskens

Saris

Creemers

2017

Cartilage

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Response to: cytokine profile of autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection - authors' reply

Cited by 5 publications

References 8 publications

The Effect of Autologous Protein Solution on the Inflammatory Cascade in Stimulated Equine Chondrocytes

The Effect of Autologous Protein Solution on the Inflammatory Cascade in Stimulated Equine Chondrocytes

A Roadmap of In Vitro Models in Osteoarthritis: A Focus on Their Biological Relevance in Regenerative Medicine

Pro- and Anti-inflammatory Cytokine Profiles in Osteoarthritis

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