Response to Comment on &amp;ldquo;The role of wildlife (wild birds) in the global transmission of antimicrobial resistance genes&amp;rdquo;

Wang, Jing; Zhi-gang, MA; Zeng, Zhenling; Yang, Xue-Wen; Huang, Ying; Liu, Jianhua

doi:10.24272/j.issn.2095-8137.2017.024

Cited by 135 publications

(147 citation statements)

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“…Enterobacteria producing ESBL have already been found on all continents, and the most frequently encountered is E. coli, followed by K. pneumoniae [47]. However, blaCTX-M beta-lactam resistance genes are the most common in the human and veterinary strains [48][49][50]. blaCTX-M-15 is the most prevalent ESBL gene in human samples worldwide [25] and is probably the most widely distributed ESBL gene in human strains around the world [23].…”

Section: Discussionmentioning

confidence: 99%

Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

et al. 2020

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In Gabon, terrestrial mammals of protected areas have been identified as a possible source of antibiotic-resistant bacteria. Some studies on antibiotic resistance in bats have already been carried out. The main goal of our study was to detect extended-spectrum beta-lactamases (ESBLs) that are produced by enterobacteria from bats in the Makokou region in Gabon. Sixty-eight fecal samples were obtained from 68 bats caught in the forests located 1 km from the little town of Makokou. After culture and isolation, 66 Gram-negative bacterial colonies were obtained. The double-disk diffusion test confirmed the presence of ESBLs in six (20.69%) Escherichia coli isolates, four (13.79%) Klebsiella pneumoniae isolates, and one (3.45%) Enterobacter cloacae isolate. The analysis based on the nucleotide sequences of the ESBL resistance genes showed that all cefotaximase-Munichs (CTX-Ms) were CTX-M-15 and that all sulfhydryl variables (SHVs) were SHV-11: 41.67% CTX-M-15-producing E. coli, 16.67% CTX-M-15+SHV-11-producing E. coli, 8.33% CTX-M-15-producing K. pneumoniae, 25% CTX-M-15+SHV-11-producing K. pneumoniae, and 8.33% CTX-M-15-produced E. cloacae. This study shows for the first time the presence of multiresistant ESBL-producing enterobacteria in fruit bats in Makokou.

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Section: Discussionmentioning

confidence: 99%

Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

et al. 2020

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“…BLAST homology analysis demonstrated that the sequence of pHN15978 showed 99% identity and 100% query coverage with E. coli strain AR_452 plasmid unnamed1 (CP030329.1) and FDAARGOS_448 plasmid unnamed1 (CP023959.1), with only 3-bp and 5-bp nucleotide differences, respectively ( Figure 1B In addition, international travel and migratory birds might also be responsible for the global dissemination of this F36:A-:B32 plasmid. 23,24 The multidrug resistance region of pHN15978 was mainly composed of three mobile modules ( Figure 1C). The first part harbored β-lactam and macrolide resistance genes.…”

Section: Resultsmentioning

confidence: 99%

Emergence of Almost Identical F36:A-:B32 Plasmids Carrying blaNDM-5 and qepA in Escherichia coli from Both Pakistan and Canada

Baloch¹,

Lv²,

Yi³

et al. 2019

IDR

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The New Delhi Metallo-β-lactamase (NDM) producing Enterobacteriaceae is spreading worldwide. Although the bla NDM gene has been identified in animal associated Enterobacteriaceae isolates in many countries, little is known about its occurrence in animal products in Pakistan. In this study, 13 Escherichia coli isolates were collected from chicken meat samples in Pakistan. Two isolates, 15978 and C4109, exhibited reduced susceptibility (MIC ≥1 μg/mL) to imipenem, and carried bla NDM-5 and bla NDM-7 gene, respectively. Whole-genome sequencing and Oxford Nanopore MinION sequencing revealed that 15978 and C4109 belonged to ST156 and ST167, respectively. bla NDM-7 was carried by an IncX3 plasmid that has disseminated worldwide, whereas bla NDM-5 was located on an F36: A-: B32 plasmid, which shared high identity with two plasmids carried by E. coli isolates from other countries (one from a patient in Canada). To the best of our knowledge, this is the first report characterizing bla NDM -carrying plasmids from chicken meat samples in Pakistan. The dissemination of almost identical bla NDM-5 -bearing F36:A-:B32 and bla NDM-7 -bearing IncX3 plasmids in different countries highlights the importance of international trade and travel in the spread of antimicrobial resistance strains and plasmids worldwide.

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“…Wild birds are also potential reservoirs of resistant bacteria and have the potential to transmit antibiotic resistance, including clinically important resistance genes. During the last two decades, different studies have been published in Europe regarding antimicrobial resistance among wild animals (Costa et al ., 2008; Poeta et al ., 2008; Radhouani et al ., 2012; Gonçalves et al ., 2014; Dias et al ., 2015; Alcala et al ., 2016; Wang et al ., 2017). Wildlife are not directly exposed to clinically useful antimicrobial agents, but they can acquire resistant bacteria mainly through water polluted from faeces of human and farm activity (Mora et al ., 2014).…”

Section: Introductionmentioning

confidence: 99%

Molecular diversity of Extended‐spectrum β‐lactamase‐producing Escherichia coli from vultures in Canary Islands

Carvalho

Tejedor-Junco

González-Martín

et al. 2020

Environ Microbiol Rep

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Antimicrobial resistance among isolates from wild animals is increasingly reported. Extended‐spectrum beta‐lactamase (ESBL)‐producing Enterobacteriaceae, and particularly Escherichia coli, have spread worldwide as one of the most common multidrug‐resistant organisms. The aim of this study was to determine the carriage rate of ESBL‐producing E. coli isolates and their genetic characteristics in wild vultures from the Canary Islands. Faecal samples were collected from 22 apparently healthy free‐ranging (wild) vulture chicks from Lanzarote and Fuerteventura (Canary Islands) during July 2019. They were seeded in MacConkey agar supplemented with cefotaxime (2 μg ml−1). Colonies with typical morphology of E. coli were identified by MALDI‐TOF‐MS. Antimicrobial susceptibility was done by disk diffusion. Phenotypic detection of ESBL was performed by double‐disk tests. The presence of blaCTX‐M, blaSHV, blaTEM, blaKPC and blaOXA‐48 genes, as well as mcr‐1 (colistin resistance), tetA/tetB and int1 gene, was tested by PCR/sequencing. Phylogenetic groups and multilocus sequence typing (MLST) were determined by PCR/sequencing. ESBL‐producing E. coli isolates were detected in 5/22 tested animals (22.7%), and all isolates (one/animal) carried blaCTX‐M genes: blaCTX‐M‐15 (n = 3) and blaCTX‐M‐55 (n = 2). ESBL‐positive isolates were ascribed to phylogenetic group D (two isolates), B1 (two isolates) and A (one isolate), and five sequence types were detected (ST/phylogenetic‐group/ESBL): ST515/B1/CTX‐M‐15, ST1290/A/CTX‐M‐15, ST38/D/CTX‐M‐15, ST457/D/CTX‐M‐55 and ST6448/B1/CTX‐M‐55; this suggests a genetic diversity among these isolates. Three CTX‐M‐15‐producing isolates contained the blaTEM gene and one the tetA gene. To our knowledge, this appears to be the first report of ESBL‐producing E. coli in vulture chicks from the Canary Islands.

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Response to Comment on “The role of wildlife (wild birds) in the global transmission of antimicrobial resistance genes”

Cited by 135 publications

References 4 publications

Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

Characterization of ESBL-Producing Enterobacteria from Fruit Bats in an Unprotected Area of Makokou, Gabon

<p>Emergence of Almost Identical F36:A-:B32 Plasmids Carrying <em>bla</em><sub>NDM-5</sub> and <em>qepA</em> in <em>Escherichia coli </em>from Both Pakistan and Canada</p>

Molecular diversity of Extended‐spectrum β‐lactamase‐producing Escherichia coli from vultures in Canary Islands

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