&lt;p&gt;Emergence of Almost Identical F36:A-:B32 Plasmids Carrying &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;NDM-5&lt;/sub&gt; and &lt;em&gt;qepA&lt;/em&gt; in &lt;em&gt;Escherichia coli &lt;/em&gt;from Both Pakistan and Canada&lt;/p&gt;

Baloch, Zulqarnain; Lv, Luchao; Yi, Long; Wan, Miao; Aslam, Bilal; Yang, Jun; Liu, Jianhua

doi:10.2147/idr.s236766

Cited by 15 publications

(16 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, their carriage rate of bla NDM is higher among the other carbapenem-resistant genes [ 35 ]. The finding of NDM-bearing IncX3 in K. pneumoniae in the present study and E. coli in other studies from Pakistan suggests the distinctive role of IncX3 plasmids in intergeneric bla NDM-1 transmission [ 32 ].…”

Section: Discussionsupporting

confidence: 75%

“…To the best of our knowledge, this is the first study from CP-KP clinical isolates in Pakistan that has bla NDM-1 -bearing IncX3 plasmids. Only one study from Pakistan demonstrated the MBL gene, i.e., NDM-7 occurrence on IncX3 plasmids in E. coli isolated from Chicken meat samples [ 32 ]. However, IncX3 bearing NDM genes are prevalent in other regions of the world, have a limited host range, and are mainly observed in Enterobacteriaceae [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

First Report of blaNDM-1 Bearing IncX3 Plasmid in Clinically Isolated ST11 Klebsiella pneumoniae from Pakistan

et al. 2021

View full text Add to dashboard Cite

The New Delhi Metallo-β-lactamase (NDM) is among the most threatening forms of carbapenemases produced by K. pneumoniae, well-known to cause severe worldwide infections. The molecular epidemiology of blaNDM-1-harboring K. pneumoniae is not well elucidated in Pakistan. Herein, we aim to determine the antibiotics-resistance profile, genes type, molecular type, and plasmid analysis of 125 clinically isolated K. pneumoniae strains from urine samples during July 2018 to January 2019 in Pakistan. A total of 34 (27.2%) K. pneumoniae isolates were carbapenemases producers, and 23 (18.4%) harbored the blaNDM-1 gene. The other carbapenemases encoding genes, i.e., blaIMP-1 (7.2%), blaVIM-1 (3.2%), and blaOXA-48 (2.4%) were also detected. The Multi Locus Sequence Typing (MLST) results revealed that all blaNDM-1-harboring isolates were ST11. The other sequence types detected were ST1, ST37, and ST105. The cluster analysis of Xbal Pulsed Field Gel Electrophoresis (PFGE) revealed variation amongst the clusters of the identical sequence type isolates. The blaNDM-1 gene in all of the isolates was located on a 45-kb IncX3 plasmid, successfully transconjugated. For the first time, blaNDM-1-bearing IncX3 plasmids were identified from Pakistan, and this might be a new primary vehicle for disseminating blaNDM-1 in Enterobacteriaceae as it has a high rate of transferability.

show abstract

Section: Discussionsupporting

confidence: 75%

Section: Discussionmentioning

confidence: 99%

First Report of blaNDM-1 Bearing IncX3 Plasmid in Clinically Isolated ST11 Klebsiella pneumoniae from Pakistan

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The ST156 and ST405 having bla NDM-1 are previously detected from chicken meat and clinical isolates. 33 , 48 ST156 and ST405 bearing mcr-1 have been detected in other countries like ST156 in Brazil and China and ST405 in Algeria and United States. 49–52 ST156 and ST405 belong to avian-pathogenic E. coli lineage and extra-intestinal pathogenic E. coil .…”

Section: Discussionmentioning

confidence: 94%

Molecular Epidemiology of mcr-1, blaKPC-2, and blaNDM-1 Harboring Clinically Isolated Escherichia coli from Pakistan

Bilal¹,

Rehman²,

Khan³

et al. 2021

IDR

View full text Add to dashboard Cite

Purpose The multiple-drug resistant Escherichia coli are among the deadliest pathogens causing life-threatening infections. This study was planned to determine the molecular epidemiology of mcr-1, bla KPC-2 , and bla NDM-1 harboring clinically isolated E. coli from Pakistan. Methods In total, 545 strains of E. coli from clinical samples were collected from June 2018 to September 2019. All the isolates were screened for colistin-resistance, extended-spectrum-β-lactamases (ESBL), and carbapenemases through the micro-dilution method, Double-Disk-Synergy-Test (DDST), and Modified-Hodge-Test (MHT). The detection, sequence-typing, conjugal transfer, S1-PFGE, plasmid-replicon-typing, and southern-blotting for mcr , ESBL, and carbapenemase-encoding genes were performed. Findings A total of four (0.73%) colistin-resistant strains carrying alongside mcr-1 and bla CTX-M-15 genes, three of these strains also had the bla TEM-1 gene. The presence of ESBL genes was detected in 139 (25.5%) isolates harboring bla CTXM-15 (74.82%), bla TEM (34.53%), bla SHV (28.06%) and bla OXA-1 (28.78%). In 129 carbapenemase-producers, 35.83% possessed bla NDM-1 , 26.67% bla KPC-2 , 8.3% bla OXA-48 , 25% bla VIM-1 , and 20.83% bla IMP-1 genes. The sequence typing revealed that mcr-1 harboring isolates belonged to ST405, ST117, and ST156. Fifty percent of bla KPC-2 and 48.83% of bla NDM-1 were found on ST131 and ST1196, respectively. Two rare types of STs, ST7584, and ST8671 were also identified in this study. The mcr-1 gene was located on Incl2 (60-kb) plasmid. The bla KPC-2 was present on (140-kb) IncH12, (100-kb) IncN, (90-kb) Incl1, while bla NDM-1 was located on (70-kb) IncFIIK, (140-kb) IncH12, (100-kb) IncN, (60-kb) IncA/C, and (45-kb) IncFII plasmids, which were successfully trans-conjugated. Among the plasmid types, the Incl1 carrying bla KPC-2 , IncH12 harboring bla KPC-2 and bla NDM-1 , and IncFIIK carrying bla NDM-1 ...

show abstract

“…Other transposable elements have been identified in GNB in Africa; in Nigeria, Tn 5403 truncated IS 3000 in K. quasipneumoniae isolates; 130 interestingly, this genetic structure was also detected in bla NDM‐7 –positive E. coli isolated from a food sample in Pakistan 188 . Also, in South Africa four transposable elements were detected, namely, TniB NTP–binding protein; TniA putative transposase, resolvase, or integrase; TinR protein; and TniQ in K. pneumoniae 79 .…”

Section: Carbapenemase‐associated Mgesmentioning

confidence: 84%

Genetic support of carbapenemases: a One Health systematic review and meta‐analysis of current trends in Africa

Ragheb

Govinden

Sekyere

2021

Annals of the New York Academy of Sciences

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) is a public health threat globally. Carbapenems are β‐lactam antibiotics used as last‐resort agents for treating antibiotic‐resistant infections. Mobile genetic elements (MGEs) play an important role in the dissemination and expression of antimicrobial resistance genes (ARGs), including the mobilization of ARGs within and between species. The presence of MGEs around carbapenem‐hydrolyzing enzymes, called carbapenemases, in bacterial isolates in Africa is concerning. The association between MGEs and carbapenemases is described herein. Specific plasmid replicons, integrons, transposons, and insertion sequences were found flanking specific and different carbapenemases across the same and different clones and species isolated from humans, animals, and the environment. Notably, similar genetic contexts have been reported in non‐African countries, supporting the importance of MGEs in driving the intra‐ and interclonal and species transmission of carbapenemases in Africa and globally. Technical and budgetary limitations remain challenges for epidemiological analysis of carbapenemases in Africa, as studies undertaken with whole‐genome sequencing remained relatively few. Characterization of MGEs in antibiotic‐resistant infections can deepen our understanding of carbapenemase epidemiology and facilitate the control of AMR in Africa. Investment in genomic epidemiology will facilitate faster clinical interventions and containment of outbreaks.

show abstract

<p>Emergence of Almost Identical F36:A-:B32 Plasmids Carrying <em>bla</em><sub>NDM-5</sub> and <em>qepA</em> in <em>Escherichia coli </em>from Both Pakistan and Canada</p>

Cited by 15 publications

References 26 publications

First Report of blaNDM-1 Bearing IncX3 Plasmid in Clinically Isolated ST11 Klebsiella pneumoniae from Pakistan

First Report of blaNDM-1 Bearing IncX3 Plasmid in Clinically Isolated ST11 Klebsiella pneumoniae from Pakistan

Molecular Epidemiology of mcr-1, blaKPC-2, and blaNDM-1 Harboring Clinically Isolated Escherichia coli from Pakistan

Genetic support of carbapenemases: a One Health systematic review and meta‐analysis of current trends in Africa

Contact Info

Product

Resources

About