Response surface methodology study of the combined effects of temperature, pH, andawon the growth rate ofTrichoderma asperellum

Begoude, B.A.D.; Lahlali, Rachid; Friel, D.; Tondje, P.R.; Jijakli, M. Haïssam

doi:10.1111/j.1365-2672.2007.03305.x

Cited by 47 publications

(35 citation statements)

References 28 publications

(53 reference statements)

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“…This strain was isolated from a naturally-infected cacao pod, collected in a trial plot in Eloumden (Yaounde, Cameroon). Strain PR11 of T. asperellum [Genebank number EF186002] was isolated in Cameroon from a soil sample taken from within the rhizosphere of cocoyam plants (Xanthosoma sagittifolium) with symptoms of root rot and conserved in national and international collections as described in Begoude et al, (2007). This strain was characterized molecularly (Samuels et al, 2010), and its antagonistic activity in relation to P. megakarya and Pythium myriotylum -the pathogens respectively responsible for cacao black pod disease and cocoyam root rot disease -was ascertained (Mbarga et al, 2012;Tondje et al, 2007).…”

Section: Fungal Materialsmentioning

confidence: 99%

A new oil-based formulation of Trichoderma asperellum for the biological control of cacao black pod disease caused by Phytophthora megakarya

et al. 2014

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HIGHLIGHTS• New oil-based dispersion with a half-life of T. asperellum conidia of 22.5 weeks.• Complete inhibition of P. megakarya on sprayed detached pods.• Enhanced rate and duration of protection on sprayed cacao pods in the field.• 50% of pods protected 3.2 weeks after spraying in the field.• Formulation suitable for the spraying equipment of small-scale cacao producers. GRAPHICAL ABSTRACTFormulated conidia of Trichoderma provide similar or even better protection of cacao pods against black pod disease than a conventional fungicide. ABSTRACTIn African cacao-producing countries, control of cacao black pod disease caused by Phytophthora megakarya is a priority. Introducing biological control agents as part of a P. megakarya control strategy is highly desirable, especially in a perspective of pesticide reduction. Trichoderma species are among the most used biological control agents. In Cameroon, Trichoderma asperellum formulated in wettable powder has produced positive effects against this disease. However, with this type of formulation, shelf-life and persistence of conidia on pods are limited. Our study therefore sought to develop a new T. asperellum formulation that would be more effective and better suited to the conditions of field application by small-scale producers in Cameroon. We selected a soybean oil-based oil dispersion, in which the half-life of the conidia reached 22.5 weeks, versus 5 weeks in Published in : Biological Control (2014), vol. 77, pp. 15-22 Status : Postprint (Author's version) aqueous suspension. Tested on detached pods, the formulation completely inhibited the development of the disease. When sprayed in the field on cacao clones highly sensitive to P. megakarya, the formulation resulted in 90% protection of treated pods after 1 week, and 50% after 3.2 weeks. The formulations exercised a measurable effect for up to 7 weeks, versus 2 weeks in the case of an aqueous conidial suspension and 5 weeks for that of a conventional fungicide (Kocide). Trichoderma asperellum formulated in oil dispersion has therefore great potential for the control of cacao black pod disease with less recourse to synthetic fungicides.

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Section: Fungal Materialsmentioning

confidence: 99%

A new oil-based formulation of Trichoderma asperellum for the biological control of cacao black pod disease caused by Phytophthora megakarya

et al. 2014

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“…Capacidad antagónica.-Pocos datos están disponibles en la literatura sobre el modelaje del crecimiento de Trichoderma spp., bajo diversas condiciones (Begoude et al 2007 Es decir, ratifican el supuesto de normalidad de residuos (Bortz 2010, Cañadas et al 2014). …”

Section: Crecimiento Micelial Deunclassified

“…Además, los modelos matemáticos han sido desarrollados para determinar el comportamiento de Trichoderma asperlleum bajo diversas condiciones ambientales, describiendo la función matemática del grado de crecimiento radial micelial (mm día-1) a través de una función sigmoidal (Begoude et al 2007). Un enfoque similar ha sido utilizado en la determinación del proceso de deterioro de alimentos causado por la actividad de patógenos como Rhizopus oligosporus Saito cepa NRRL2719 y otros patógenos de poscosecha en frutos como Penicillium expansum (Lahlali et al 2005).…”

Section: Crecimiento Micelial Deunclassified

“…Los modelos matemáticos han sido aplicados para describir el comportamiento de microorganismos en diferentes medios. De acuerdo con Begoude et al (2007) el método empleado fue un modelo polinómico de segundo orden y los coeficientes fueron calculados mediante un análisis de regresión múltiple, en el cual la variable independiente corresponde al grado de crecimiento radial de Trichoderma spp., influenciado por la disponibilidad de agua, temperatura y pH. Resaltando que, aún no han sido descri-te el software SPSS Statistics 21 (Software analítico, Nueva York, USA).…”

Section: Introductionunclassified

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Caracterización in vitro de Trichoderma spp., y modelo matemático de su antagonismo en presencia de Moniliophthora perniciosa

Vélez¹,

López²,

Navarrete³

et al. 2017

REMCB

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Para optimizar el uso práctico de un agente de control biológico es esencial entender cómo las condiciones físicas afectan la sobrevivencia, crecimiento y reproducción de estos agentes. Trichoderma ha sido descrito como un agente de control biológico eficaz. El objetivo de la presente investigación fue realizar la caracterización morfológica, determinación del crecimiento y el establecimiento de la capacidad antagónica de aislados nativos de Trichoderma spp., a nivel in vitro frente al fitopatógeno Moniliophthora perniciosa (ex Crinipellis perniciosa) a través de modelos matemáticos. Esta investigación fue conducida en la Estación Experimental Portoviejo (EEP) del Instituto Nacional de Investigaciones Agropecuarias (INIAP). Consecuentemente, se identificaron 12 aislados de Trichoderma spp., en función de sus características morfológicas. El aislado A1 que, mostró un fuerte aroma a coco, una alta tasa de crecimiento inicial en cultivo puro y una morfología plana, resultó ser el más efectivo a la hora de inhibir el desarrollo de M. perniciosa. La determinación de modelos matemáticos que describen el crecimiento micelial de M. perniciosa ante la presencia antagónica de los aislados de Trichoderma spp., permitiría una rápida identificación, caracterización de aislados de Trichoderma spp., y su aplicación práctica.

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“…The effect of low temperatures , water activity and pH (Kredics et al 2000;Kredics et al 2004;Begoude et al 2007), or the presence of heavy metals, or pesticides or antagonistic bacteria (Kay and Stewart, 1994;Naár and Kecskés, 1998;Kredics et al 2001) have been tested as stress factors that may affect wild or mutant biocontrol Trichoderma strains. Nevertheless, most of these studies have been run in in vitro conditions of the biocontrol agent.…”

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confidence: 99%

Biocontrol capacity of wild and mutant Trichoderma harzianum (Rifai) strains on Rhizoctonia solani 618: effect of temperature and soil type during storage

Montealegre¹,

Valderrama²,

Herrera³

et al. 2009

Electro Journal of Biotech

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Wild (Th11, Th12 and Th650) and mutant (Th11A80.1, Th12A40.1, Th12C40.1 and Th650-NG7) Trichoderma harzianum strains were stored for 180 days at 5ºC or at 22ºC, in two types of soils. Strains recovered at 90, 120 and 180 days from the two types of soils, retained their full capacity to biocontrol Rhizoctonia solani 618, that produces crown and root rot of tomatoes. Recovery, estimated as colony forming units (cfu) of the wild and mutant strains, showed that all increased their cfu after storage independently of the type of soil and temperature, although kinetic behavior differed among strains. Ratios of recovery after storage in type B soil/ type A soil or at 22ºC/5ºC, higher or lower than one respectively, allowed to establish that Th11 and Th12 were the most appropriate strains for the biocontrol of R. solani in conditions where growth of the phytopathogen is optimal.

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Response surface methodology study of the combined effects of temperature, pH, anda_won the growth rate ofTrichoderma asperellum

Cited by 47 publications

References 28 publications

A new oil-based formulation of Trichoderma asperellum for the biological control of cacao black pod disease caused by Phytophthora megakarya

A new oil-based formulation of Trichoderma asperellum for the biological control of cacao black pod disease caused by Phytophthora megakarya

Caracterización in vitro de Trichoderma spp., y modelo matemático de su antagonismo en presencia de Moniliophthora perniciosa

Biocontrol capacity of wild and mutant Trichoderma harzianum (Rifai) strains on Rhizoctonia solani 618: effect of temperature and soil type during storage

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