Response surface methodology for optimization and characterization of limonene-based coenzyme Q10 self-nanoemulsified capsule dosage form

Palamakula, Anitha; Nutan, Mohammad T. H.; Khan, Mansoor A.

doi:10.1208/pt050466

Cited by 52 publications

(29 citation statements)

References 17 publications

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“…20 According to reviewed studies, the following variables were selected: polymer concentration, ratio of oil phase to water phase, and emulsifier concentration. For each variable, three levels were chosen for Box-Behnken experiment design, as shown in Table 1.…”

Section: Experimental Designmentioning

confidence: 99%

Cooperation of IRAK1/4 inhibitor and ABT-737 in nanoparticles for synergistic therapy of T cell acute lymphoblastic leukemia

Wu¹,

Wang²,

Qiu³

et al. 2017

IJN

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T cell acute lymphoblastic leukemia (T-ALL) is caused by clonal expansion of variant T cell progenitors and is considered as a high risk leukemia. Contemporary single chemotherapy has a limited effect due to dynamic and versatile properties of T-ALL. Here IRAK1/4 inhibitor and ABT-737 were co-encapsulated into polyethylene glycol modified poly (lactic-co-glycolic acid) nanoparticles (IRAK/ABT-NP) to enhance synergistic therapy of T-ALL. The formulation was optimized to achieve high drug loading using Box-Behnken design and response surface methodology. The optimal parameter comprised 2.98% polymer in acetonitrile, a ratio of oil phase to water phase of 1:8.33, and 2.12% emulsifier concentration. High drug loading and uniform spherical shape was achieved. In vitro release study showed sustained release of IRAK1/4 inhibitor for 72 hours as well as sustained release of ABT-737 for more than 120 hours. Uptake efficiency of IRAK/ABT-NP and induced apoptotic T-ALL fraction by IRAK/ABT-NP were much higher than the IRAK1/4 and ABT-737 combined solution. IC 50 of IRAK/ABT-NP was two-fold lower than free drug combination in Jurkat cells. Additionally, we conducted in vivo experiments in which IRAK/ABT-NP exhibited greater cytotoxicity toward T-ALL cells, the capacity to significantly restore white blood cell number in peripheral blood, and improved survival time of T-ALL mouse model compared to the IRAK1/4 and ABT-737 combined solution.

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Section: Experimental Designmentioning

confidence: 99%

Cooperation of IRAK1/4 inhibitor and ABT-737 in nanoparticles for synergistic therapy of T cell acute lymphoblastic leukemia

Wu¹,

Wang²,

Qiu³

et al. 2017

IJN

View full text Add to dashboard Cite

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“…Experiments were run in random order to increase the predictability of the model. Optimisation was performed using a desirability function to obtain the levels of X1, X2 and X3, which minimised (Y1) and (Y3) and maximised (Y2) [16][17][18][19][20][21]. The quadratic model generated by design is as follows:…”

Section: Experimental Designmentioning

confidence: 99%

Design, Optimization and in Vitro Characterization of Self Nano Emulsifying Drug Delivery System of Olmesartan Medoxomil

Sisinthy

Nalamolu

Sarah

2016

Int J Pharm Pharm Sci

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Objective: The objective of the present study was to design, optimise and characterise self nano emulsifying drug delivery systems (SNEDDS) for a poorly water soluble drug, olmesartan medoxomil (OLM) by Formulation by Design (FbD) approach with an aim to improve its solubility and dissolution. Methods:The SNEDDS were systematically optimised using three factor Box-Behnken Results: Following optimisation, the values of formulation variables were found to be 142.276 mg (Capryol P), 399.999 mg (Cremophor EL) and 598.871 mg (Transcutol P) which produced a globule size of 12.64 nm, percentage drug release of 93.34% and a turbidity of 0.02 FNU. TEM studies demonstrated spherical droplet morphology.design. Concentration of formulation variables, namely, the oil phaseX1 (Capryol 90), the surfactant X2 (Cremophor EL), and the co-surfactant X3 (Transcutol P), was optimized for its impact on mean globule size (Y1), percentage drug release in 20 min (Y2) and turbidity (Y3) of the formulation. Ternary phase diagrams were constructed to select the areas of nanoemulsion and the amounts of oil, surfactant and cosurfactants as critical formulation variables. The prepared SNEDDS were characterised for globule size, dissolution studies, turbidity, and transmission electron microscopy (TEM). Conclusion:Thus, the present studies reveal that the SNEDDS is a promising drug delivery system approach for the enhancement of solubility and dissolution rate of OLM.

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“…Five grams of the soil in the above-mentioned four batches was added to 50 mL of aqueous solution containing 1.6 mg/L of Vapam. [14] Each of the four solutions was then subjected to different values of initial pH of the fungicide solution (2)(3)(4)(5)(6)(7)(8), temperature (15-35 • C) and shaking time (2-24 h). Note that the adsorption of anions (anionic pesticides) was favored at pH< pH ZPC (pH at the potential of zero charge) [15] ; therefore, the effect of initial pH of the fungicide solution was studied under pH = 8 according to Table 2.…”

Section: Adsorption Experimentsmentioning

confidence: 99%

“…Among all the RSM designs, Box Behnken Design (BBD) requires fewer runs in a 4-factor experimental design. [8,9] Therefore, in this study, the sorption process of dithiocarbamate pesticide "Vapam" onto soil modified with perlite was investigated and the BBD was used to determine the optimal conditions of the pesticide sorption.…”

Section: Introductionmentioning

confidence: 99%

A Box-Behnken design for determining the optimum experimental condition of the fungicide (Vapam) sorption onto soil modified with perlite

Azizi

Asemi

2012

Journal of Environmental Science and Health, Part B

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In the present study, response surface methodology (RSM) based on the Box-Behnken design (BBD) was employed to investigate the effects of the different operating conditions on the removal of the fungicide (Vapam) onto soil modified with perlite using sorption process. The process parameters such as pH of the fungicide solution (2, 5 and 8), temperature (15, 25 and 35°C), shaking time (2, 13 and 24 h) and the percentage of perlite in the modified soil (0, 2 and 4 %) were investigated using a four-factor-three-level Box-Behnken design at an initial fungicide concentration of C(0) = 1.6 mg/L as a fixed input parameter. A second-order quadratic model suggested the optimum conditions to be as follows: fungicide solution pH of 3.57, temperature of 15°C, shaking time of 3.5 h and 4% of perlite in the modified soil which resulted in the improvement of Vapam sorption. Under optimum conditions, the fungicide (Vapam) removal was predicted 12.88 μg/g by BBD. The confirmatory experiments were conducted and the results revealed that the fungicide removal was 13.14 μg/g which indicated that the predicted and the observed values of response (Vapam removal) were in close agreement. Therefore, the soil modified with perlite holds good potential for Vapam sorption. This is the first report on fungicide Vapam sorption onto soil modified with perlite using statistical experimental design employing response surface methodology.

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Response surface methodology for optimization and characterization of limonene-based coenzyme Q10 self-nanoemulsified capsule dosage form

Cited by 52 publications

References 17 publications

Cooperation of IRAK1/4 inhibitor and ABT-737 in nanoparticles for synergistic therapy of T cell acute lymphoblastic leukemia

Cooperation of IRAK1/4 inhibitor and ABT-737 in nanoparticles for synergistic therapy of T cell acute lymphoblastic leukemia

Design, Optimization and in Vitro Characterization of Self Nano Emulsifying Drug Delivery System of Olmesartan Medoxomil

A Box-Behnken design for determining the optimum experimental condition of the fungicide (Vapam) sorption onto soil modified with perlite

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