Response surface method optimization of ectoine fermentation medium with moderate halophilic bacteria<i>Halomonas</i>sp. H02

Li, T T; Qu, A; Yuan, Xiangjuan; Tan, F X; Li, X W; Wang, T; Zhang, L H

doi:10.1088/1755-1315/77/1/012019

Cited by 6 publications

(1 citation statement)

References 9 publications

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“…The predicted optimal ectoine production was 55 mg/g.h at NaCl concentration 14.9% and initial pH 9.0 and incubation temperature 40°C. Li et al (2017) succeeded to increase ectoine yield of Halomonas sp. HO 2 by 41.6% after optimizing initial pH, sodium glutamate concentration and salinity using response surface methodology.…”

Section: Optimizing Of Ectoine Production By a Central Composite Design Modelmentioning

confidence: 99%

Optimizing ectoine biosynthesis using response surface methodology and osmoprotectant applications

et al. 2020

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Purpose Numerous applications of compatible salts (osmolytes) as ectoine in food and pharmaceutical industries have been intensively increased nowadays. Decreasing the cost of industrial production of ectoine using low-cost cultivation media and improving the yield through modeling procedures are the main scopes of the present study. Methods Three statistical design experiments have been successfully applied for screening the parameters affecting the production process, studying the relations among parameters and optimizing the production using response surface methodology. Results A novel semi-synthetic medium based on hydrolyzed corn gluten meal has been developed to cultivate moderate halophilic bacterial strains; Vibrio sp. CS1 and Salinivibrio costicola SH3, and support ectoine synthesis under salinity stress. Two regression equations describe the production process in the new medium have been formulated for each bacterial strain. Response surface optimizer of the central composite model predicts the maximum ectoine production is achieved at incubation time; 63.7 h, pH; 7.47 and salinity; 7.27% for Vibrio sp. CS1 whereas these variables should be adjusted at 56.95 h, 7.089 and 10.34%; on the same order regarding Salinivibrio costicola SH3. In application studies, 50 lg ectoine decreases RBCs hemolysis due to streptolysin O toxin by 21.7% within ten minutes. In addition, 2% ectoine succeeds to increase the viability of lactic acid bacteria in Yogurt as a classic example of functional food during the storage period (7 days). Conclusion The present study emphasizes on modeling the process of ectoine production by halophilic bacteria as well as its activity as a cryoprotectant agent. Keywords Moderate halophiles Á Ectoine Á Full factorial design Á Central composite design Á Streptolysin O toxin Á Lactic acid bacteria

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Section: Optimizing Of Ectoine Production By a Central Composite Design Modelmentioning

confidence: 99%

Optimizing ectoine biosynthesis using response surface methodology and osmoprotectant applications

et al. 2020

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Compatible Solute Ectoines: Fancy Marine Product for Pharmaceuticals and Cosmeceuticals

Andharia

Kothari

2020

Marine Niche: Applications in Pharmaceutical Sciences

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Identification of novel halophilic/halotolerant bacterial species producing compatible solutes

Orhan

Ceyran

2022

Int Microbiol

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Response surface method optimization of ectoine fermentation medium with moderate halophilic bacteriaHalomonassp. H02

Cited by 6 publications

References 9 publications

Optimizing ectoine biosynthesis using response surface methodology and osmoprotectant applications

Optimizing ectoine biosynthesis using response surface methodology and osmoprotectant applications

Compatible Solute Ectoines: Fancy Marine Product for Pharmaceuticals and Cosmeceuticals

Identification of novel halophilic/halotolerant bacterial species producing compatible solutes

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