Response of physiological characteristics and grain yield of winter wheat varieties to long-term heat stress at anthesis

Gao, Chong; Sun, Mengzhe; Anwar, Sumera; Feng, Bo; Ren, A.; Lin, Wang; Gao, Zhen

doi:10.32615/ps.2021.060

Cited by 4 publications

(1 citation statement)

References 47 publications

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“…Both the increased and decreased CAT activity in plants of different species, including wheat, under heat stress has been reported in the literature. Meanwhile, higher values of the enzyme activity were observed in resistant varieties, moreover at different phases of plant development (Hameed et al, 2012;Gao et al, 2021). An increased expression of catalase genes, especially CAT2, under severe drought and hyperthermia has been reported (Laxa, 2019).…”

Section: Fig 6 Correlation Coefficients Between Growth Inhibition And...mentioning

confidence: 96%

Growth responses of wheat seedlings of different varieties to heat-stress and their relation to the antioxidant system state and osmolytes accumulation

Kolupaev¹,

Маkaova²,

Yastreb³

et al. 2023

Biol. Stud.

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Background. In recent decades, Ukraine has been experiencing abnormally high temperatures and droughts in different seasons, including autumn. This creates stressful conditions for winter cereals, especially wheat, at the very beginning of ontogeny. A comprehensive study of the functioning of antioxidant and osmoprotective systems in the early stages of development for wheat varieties of different ecological and geographical origins has not been conducted yet. This study aimed to investigate the effect of heat stress on the growth of etiolated seedlings of seven varieties of winter wheat (Triticum aestivum L.) and the indicators characterizing the functioning of antioxidant and osmoprotective systems. Materials and Methods. Wheat grains of different varieties were germinated at 24 °C for three days in the dark. Subsequently, they were subjected to 4 h of heating at 45 °C in an air thermostat. Immediately after stress, the generation of superoxide anion radical (O2•‒) by seedlings, the content of hydrogen peroxide (H2O2), lipid peroxidation (LPO) products, catalase and guaiacol peroxidase activity, and also proline and soluble carbohydrates were analyzed. One day after heating, the relative inhibition of shoot and root growth was determined. Results and Discussion. The Antonivka and Tobak varieties demonstrated the highest ability to maintain growth after exposure to high temperatures; the Darynka Kyivska and Lira Odeska varieties were medium resistant. In the Doskonala, Bogdana and Avgustina varieties, a strong inhibition of shoot and root growth after heat stress was noted. In Antonivka, after high-temperature exposure, the O2•‒ generation increased slightly, while the content of H2O2 and LPO products did not change. In Tobak, Darynka Kyivska and Lira Odeska, the stress-induced increase in ROS formation and accumulation of LPO products was moderate. The hyperthermia-sensitive Doskonala, Bogdana and Avgustina showed a significant development of oxidative stress. Heat-resistant varieties had high catalase activity and increased total soluble carbohydrate content under heat stress. After exposure to high temperature, the proline content increased in all studied varieties, but no positive correlation was recorded between its amount and their heat resistance. Likewise, no significant correlation was found between the guaiacol peroxidase activity and the ability of varieties to maintain growth after heat stress. Conclusion. The high-level inverse correlation between catalase activity, soluble carbohydrate content, and the manifestation of oxidative stress effect indicates a significant contribution of these stress-protective system components to the adaptation of seedlings to heat stress. Indicators of the oxidative stress intensity and the functioning of individual components of the antioxidant system can be used to assess the heat resistance of wheat varieties at the early stages of development.

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Section: Fig 6 Correlation Coefficients Between Growth Inhibition And...mentioning

confidence: 96%

Growth responses of wheat seedlings of different varieties to heat-stress and their relation to the antioxidant system state and osmolytes accumulation

Kolupaev¹,

Маkaova²,

Yastreb³

et al. 2023

Biol. Stud.

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Cellular Mechanisms of the Formation of Plant Adaptive Responses to High Temperatures

et al. 2023

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Integrated transcriptome and proteome analysis reveals molecular responses of soybean anther under high-temperature stress

Chen

Zhi

et al. 2023

Front. Plant Sci.

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It is well documented that high temperature (HT) severely affects the development of soybean male reproductive organs. However, the molecular mechanism of thermo-tolerance in soybean remains unclear. To explore the candidate genes and regulatory mechanism of soybean response to HT stress and flower development, here, the anthers of two previously identified HT-tolerant (JD21) and HT-sensitive (HD14) varieties were analyzed by RNA-seq. In total, 219 (172 upregulated and 47 downregulated), 660 (405 upregulated and 255 downregulated), and 4,854 (2,662 upregulated and 2,192 downregulated) differentially expressed genes (DEGs) were identified between JD21 anthers treated with HT stress vs. JD21 anthers in the natural field conditions (TJA vs. CJA), HD14 anthers treated with HT stress vs. HD14 anthers in the natural field conditions (THA vs. CHA), and JD21 vs. HD14 anthers treated with HT stress (TJA vs. THA), respectively. The results showed that there were more DEGs upregulated in JD21; this might be the reason why JD21 was more HT-resistant than the HT-sensitive variety HD14. GO annotation and KEGG enriched analysis showed that many DEGs are mainly involved in defense response, response to biological stimuli, auxin-activated signaling pathway, plant hormone signal transduction, MAPK signaling pathway-plant, starch and sucrose metabolism, etc. The conjoint analysis of RNA-seq and previous iTRAQ results found that there were 1, 24, and 54 common DEGs/DAPs showing the same expression pattern and 1, 2, and 13 common DEGs/DAPs showing the opposite pattern between TJA vs. CJA, THA vs. CHA, and TJA vs. THA at the protein and gene level, respectively, among which HSPs, transcription factor, GSTU, and other DEGs/DAPs participated in the response to HT stress and flower development. Notably, the qRT-PCR analysis and physiological index change results coincided with the sequencing results of RNA-seq and iTRAQ. In conclusion, the HT-tolerant cultivar performed better under stress than the HT-sensitive cultivar through modulation of HSP family proteins and transcription factors, and by keeping key metabolic pathways such as plant hormone signal transduction normal. This study provided important data and some key candidate genes to better study the effect and molecular basis of HT on anther in soybean at a transcription and translation level.

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Response of physiological characteristics and grain yield of winter wheat varieties to long-term heat stress at anthesis

Cited by 4 publications

References 47 publications

Growth responses of wheat seedlings of different varieties to heat-stress and their relation to the antioxidant system state and osmolytes accumulation

Growth responses of wheat seedlings of different varieties to heat-stress and their relation to the antioxidant system state and osmolytes accumulation

Cellular Mechanisms of the Formation of Plant Adaptive Responses to High Temperatures

Integrated transcriptome and proteome analysis reveals molecular responses of soybean anther under high-temperature stress

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