Response of Peritoneal Exudate Cells to Brucella melitensis. Influence of Nature of Inflammatory Irritant.

Elberg, Sanford S.; Mascarenhas, Patricia; Fong, Jacob

doi:10.3181/00379727-104-25814

Cited by 10 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The potential problems associated with irritantinduced cells are exemplified by the work of investigators who demonstrated that, when irritants were employed to stimulate rat peritoneal exudates, the results obtained after infection of these cells varied with the different eliciting agents (12). Similar results were obtained with other experimental models (6,10).…”

mentioning

confidence: 81%

In Vitro Interactions Between Rabbit Alveolar Macrophages andPasteurella tularensis

Nutter

Myrvik

1966

J Bacteriol

View full text Add to dashboard Cite

In vitro interactions between rabbit alveolar macrophages and Pasteurella tularensis. J. Bacteriol. 92:645-651. 1966.-Rabbit alveolar macrophages were successfully employed in a study of host cell-Pasteurella tularensis interactions in vitro. Under cell culture conditions in which inhibitory antibiotics were not employed and small infection ratios were used, the relative in vivo virulence of two strains of P. tularensis was duplicated. As a consequence of intracellular multiplication, normal macrophages were killed in relation to the virulence of the strain employed. Alveolar macrophages were also collected from une rabbits, and macrophage mortality and bacterial growth were significantly suppressed below levels observed with normal macrophage preparations. The effect of iune serum could only be ascribed a minor role in the observed reactions. A marked intravenous toxicity of P. tularensis for the rabbit was observed with both virulent and attenuated strains. The toxicity was possessed only by viable preparations and could be elicited in animals immune to virulent challenge.

show abstract

mentioning

confidence: 81%

In Vitro Interactions Between Rabbit Alveolar Macrophages andPasteurella tularensis

Nutter

Myrvik

1966

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…Since MN and PMN from different rabbits in response to different irritants are not strictly comparable (cf. Elberg,37), a histochemical comparison was made of these cells when they were simultaneously present in the same 18-hour glycogen exudate. In this type of exudate, MN and PMN show high viabilities both by the dye tests (see Materials and Methods) and by their ability to phagocytize particles (3).…”

Section: Histochemical Studies On M_n and Pmnmentioning

confidence: 99%

A Histochemical Study of Phagocytic and Enzymatic Functions of Rabbit Mononuclear and Polymorphonuclear Exudate Cells and Alveolar Macrophages

Dannenberg

Burstone

Walter

et al. 1963

The Journal of Cell Biology

127

View full text Add to dashboard Cite

The cytochromc oxidasc (CO), aminopcptidasc (AMP), succinic dchydrogcnasc (SD), acid phosphatasc, cstcrasc, and alkaline phosphatasc of rabbit mononuclcar (MN) and polymorphonuclcar (PMN) peritoneal cxudatc cells and pulmonary alveolar macrophagcs (AM) -air dried on Mylar strips-were characterized by histochcmical techniques with respect to stability, activators, inhibitors, and pH optima. A granule count method was established for the quantitation of these cnzymcs. For the acid phosphatasc of MN, in which the most precise results wcrc obtained, time, pH, substratc, and inhibitor curves resembled those commonly obtained biochcmically. Five of these enzymes wcrc usually more active in AM than MN, whereas the sixth, alkaline phosphatasc, was not present in cithcr cell type. AM also tended to consume more oxygen than MN and to divide more frequently. Since the most active cells in thc population would bc first involved in the host's defense against microbial agents, a comparison was made of the 10 per cent of the AM and MN with the highest enzymatic activities. No differences wcrc found in the granule counts that wcrc not reflected by the means. However, within a given AM population, cells containing ingested dust particles sccmcd to have higher enzymatic activities than those without particles. MN had greater acid phosphatasc and SD activities than PMN and consumcd more oxygen, but the CO, AMP, and cstcrasc activitcs of both types of cells wcrc of similar magnitude. PMN showed high alkaline phosphatasc activity; MN showed none. A survcy of the histochcmical litcraturc indicates that a positive correlation between the enzymatic and phagocytic activities of both MN and PMN exists in vivo.Mononuclear phagocytes (MN) 1 are essential components of the reticuloendothelial system--the chief defense of the host against many micro organisms and their products. Similar functions i The abbreviations used in this report are as follows : MN, mononuclear(s), or mononuclear peritoneal exudate cells; PMN, polymorphonuclear(s) or polymorphonuclear peritoneal exudate cells; AM, are served by polymorphonuclears (PMN) in the blood and in areas of inflammation, and by alveolar macrophages (AM) in the lung. The present report (a) characterizes six enzymes of rabbit MN alveolar macrophage(s) of the lung; ip, intraperitoneal; CO, cytochrome oxidase; AMP, aminopeptidase; SD,succinic dehydrogenase; Tris, tris(hydroxymethyl) aminomethane. 465on

show abstract

“…In some animals, a sufficient yield of cells can be obtained without previous stimulation. This is perhaps preferable, for Elberg et al (16) claim that the outcome of infection studies can be affected by the nature of the irritant used to produce the phagocytic cells. Once the macrophages are obtained, they are usually suspended in a liquid medium and dispensed in known amounts in tubes or flasks.…”

mentioning

confidence: 99%

Interaction of Salmonella with Phagocytes in Vitro

Morello

Baker

1965

Journal of Infectious Diseases

View full text Add to dashboard Cite

Response of Peritoneal Exudate Cells to Brucella melitensis. Influence of Nature of Inflammatory Irritant.

Cited by 10 publications

References 0 publications

In Vitro Interactions Between Rabbit Alveolar Macrophages andPasteurella tularensis

In Vitro Interactions Between Rabbit Alveolar Macrophages andPasteurella tularensis

A Histochemical Study of Phagocytic and Enzymatic Functions of Rabbit Mononuclear and Polymorphonuclear Exudate Cells and Alveolar Macrophages

Interaction of Salmonella with Phagocytes in Vitro

Contact Info

Product

Resources

About