Response of non-enzymatic antioxidative mechanisms to stress caused by infection with <i>Fusarium</i> fungi and chemical protection in different wheat genotypes

Stuper‐Szablewska, Kinga; Kurasiak-Popowska, Danuta; Perkowski, J.

doi:10.1080/02757540.2017.1381689

Cited by 30 publications

(28 citation statements)

References 27 publications

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“…The inoculation methods and inoculum volumes used in this study effectively delivered infective stalk rot inoculum dosage, the rate of which were found to be srongly correlated with phenol production in maize plants at 4 th , 5 th and 6 th week after inoculation. This, agreed with the claim that majority of phenolic compounds are a part of the preformed general defense system against potential pathogens [35].…”

Section: Fig 3 Effect Of Time (Wap) On Disease Severity Of F Vertisupporting

confidence: 87%

Evaluation of the Phenol Production Potential in Maize (Zea mays L.) in Response to Infection Caused by Fusariun verticillioides (Niren.)

Oriyomi¹,

Sobowale²,

Akanmu³

et al. 2019

JEAI

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This study investigated phenol production in five maize varieties in response to infection caused by Fusarium verticillioides. Pure culture of the pathogen was obtained from Plant Pathology Laboratory of the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria. The screen house experiment was laid out in a completely randomized design. Dual inoculation was done where soil was infected separately before planting and seedlings were infected separately two weeks after planting with two volumes (10ml and 20ml) containing 1.4×107 spores/ml suspension of F. verticillioides. The maize plants were harvested at 4th, 5th and 6th weeks after inoculation and the maize plants were dried at room temperature before determination of phenol content in each of the varieties. Data gathered on the agronomic parameters and phenol contents were subjected to analysis of variance (ANOVA) using SAS 9.1 statistical package. All the maize varieties recorded more than 70% stalk rot incidence while the severity ranged from 19.01% in variety ART-98-SW1 to 25.21% in ART-98-SW6. ART-98-SW6 showed the most (p<0.05) phenol content (21.28 mg/g). Soil inoculation produced the highest percentage stalk rot severity while seedling inoculation showed significantly higher phenol contents across the period of study. Similarly, disease severity increased with increasing inoculum levels with highest attained using 20 ml (23.99%) while higher phenol content was obtained at inoculum level 10 ml (18.61 mg/g) compared to results using 20 ml (15.63 mg/g) and control (7.88 mg/g). The maize variety; ART-98-SW6 with highest stalk rot severity also produced the highest phenol content. Overall, the rate of phenol production in maize corresponded with the extent of severity F. verticillioides infections.

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Section: Fig 3 Effect Of Time (Wap) On Disease Severity Of F Vertisupporting

confidence: 87%

Evaluation of the Phenol Production Potential in Maize (Zea mays L.) in Response to Infection Caused by Fusariun verticillioides (Niren.)

Oriyomi¹,

Sobowale²,

Akanmu³

et al. 2019

JEAI

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“…The average monthly temperatures and monthly precipitation, measured according to the (World Meteorological Organization) WMO guidelines for the years 2011-2013, were obtained from The plant material was 23 winter wheat genotypes (Table S1) with different Fusarium wilt resistance, as follows: 13 wheat lines obtained from Polish plant breeding companies; seven genotypes from six European countries; and three from the Department of Genetics and Plant Breeding, the Poznań University of Life Sciences [20]. The experiment had the following two factors: the first was the variant of protection and the second was the wheat genotype.…”

Section: Site Locationmentioning

confidence: 99%

“…The ferulic acid in the samples was analyzed after alkaline and acidic hydrolysis [20]. Analysis was performed using an Aquity H class (Ultra Performance Liquid Chromatography) UPLC system equipped with a Waters Acquity PDA detector (Waters Corporation, Parsippany, NJ, USA).…”

Section: Determination Of Ferulic Acidmentioning

confidence: 99%

“…In addition to genetic studies on wheat resistance to Fusarium, research has also been conducted on the biochemical aspects of plant responses to a massive pathogen attack. Based on the literature sources [18,19] and our own [20,21] research, it was found that antioxidative processes based on the significantly increased biosynthesis of the low-molecular antioxidants of the plant have a significant impact on pathogenesis, and constitute the first line of defense against pathogens. Based on the concentration of the selected phenolic acids, including ferulic acid, it is possible to assess the degree of disease risk at an early stage of pathogenesis [20,22].…”

Section: Introductionmentioning

confidence: 99%

“…Based on the literature sources [18,19] and our own [20,21] research, it was found that antioxidative processes based on the significantly increased biosynthesis of the low-molecular antioxidants of the plant have a significant impact on pathogenesis, and constitute the first line of defense against pathogens. Based on the concentration of the selected phenolic acids, including ferulic acid, it is possible to assess the degree of disease risk at an early stage of pathogenesis [20,22]. Taking into account all of the current information on the mechanism of wheat resistance to Fusarium [20,23,24], as well as the results of chemical analyzes from three years of field experiments on wheat, it was decided to use modern methods of data analysis to determine the relationship between the concentration of ferulic acid; the concentration of deoxynivalenol and nivalenol (NIV), depending on weather conditions; and the infestation degree by fungal diseases.…”

Section: Introductionmentioning

confidence: 99%

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Application of Artificial Neural Networks to Analyze the Concentration of Ferulic Acid, Deoxynivalenol, and Nivalenol in Winter Wheat Grain

2020

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Biotic stress, which includes infection by pathogenic fungi, causes losses of wheat yield in terms of quantity and quality. Ear Fusarium is caused by strains of F. graminearum and F. culmorum, which can produce mycotoxins—deoxynivalenol (DON) and nivalenol (NIV). One of the wheat’s defense mechanisms against stressors is the activation of biosynthesis pathways of antioxidant compounds, including ferulic acid. The aim of the study was to conduct pilot studies on the basis of which neural models were created that would examine the impact of the variety and weather conditions on the concentration of ferulic acid, and link its content with the concentration of deoxynivalenol and nivalenol. The plant material was 23 winter wheat genotypes with different Fusarium resistance. The field experiment was conducted in 2011–2013 in Poland in three experimental combinations, namely: with full chemical protection; without chemical protection, but infested with natural disease (control); and in the absence of fungicidal protection, with artificial inoculation by genus Fusarium fungi. As a result of the pilot studies, three neural models—FERUANN analytical models (ferulic acid content), DONANN (deoxynivalenol content) and NIVANN (nivalenol content)—were produced. Each model was based on 14 independent features, 12 of which were in the form of quantitative data, and the other two were presented as qualitative data. The structure of the created models was based on an artificial neural network (ANN) of the multilayer perceptron (MLP) with two hidden layers. The sensitivity analysis of the neural network showed the two most important features determining the concentration of ferulic acid, deoxynivalenol, and nivalenol in winter wheat seeds. These are the experiment variant (VAR) and winter wheat variety (VOW).

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Quantitative profile of phenolic acids and antioxidant activity of wheat grain exposed to stress

Stuper‐Szablewska

Kurasiak-Popowska

Perkowski

2019

Eur Food Res Technol

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In the case of biotic and abiotic stresses, natural defence mechanisms are triggered in plants. The mechanisms are based mainly on enzymatic or non-enzymatic intracellular reactions. Changes in the quantitative profile of 12 phenolic acids, total bound phenolic acids, total free phenolic acids and antioxidant activity in grain of 23 winter wheat genotypes exposed to stress (artificial inoculation with spores of fungi from the genus Fusarium or chemical protection) were analysed. Concentrations of ergosterol confirm significantly higher levels of contamination with microscopic fungi in inoculated wheat samples in comparison to the control and chemically protected samples. The highest concentrations of bound phenolic acids were recorded in inoculated samples while the control and chemically protected samples did not differ significantly. Chromatographic analysis of contents of 12 bound phenolic acids showed that significant differences were found for contents of ferulic, synapic, t-cinnamic and 4-hydroxybenzoic acids in experimental variants. Stepwise discriminatory analysis based on the contents of selected bound phenolic acids antioxidant activity and free phenolic acids led to a complete separation of the investigated populations of the experimental groups. The statistical analysis confirmed that free phenolic acids are the most significant factors in resistance mechanisms as the first line of defence together with their antioxidant action, followed by the synergistic effect of exogenous bound phenolic acids: ferulic, synapic, t-cinnamic and 4-hydroxybenzoic acids.

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Response of non-enzymatic antioxidative mechanisms to stress caused by infection with Fusarium fungi and chemical protection in different wheat genotypes

Cited by 30 publications

References 27 publications

Evaluation of the Phenol Production Potential in Maize (Zea mays L.) in Response to Infection Caused by Fusariun verticillioides (Niren.)

Evaluation of the Phenol Production Potential in Maize (Zea mays L.) in Response to Infection Caused by Fusariun verticillioides (Niren.)

Application of Artificial Neural Networks to Analyze the Concentration of Ferulic Acid, Deoxynivalenol, and Nivalenol in Winter Wheat Grain

Quantitative profile of phenolic acids and antioxidant activity of wheat grain exposed to stress

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