Response of human pulps following acid conditioning and application of a bonding agent in deep cavities

Costa, Carlos Alberto de Souza; Nascimento, Alexandre Batista Lopes do; Teixeira, Hilcia Mezzalira

doi:10.1016/s0109-5641(01)00089-6

Cited by 65 publications

(42 citation statements)

References 28 publications

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“…The pulpal response results provided the necessary in vivo evidence to support our claim that the use of the augmented-pressure adhesive displacement technique does not generate a strong negative capillary pressure that causes displacement of odontoblast cell bodies into the dentinal tubules. It is important to highlight that tooth preparation and bonding treatment can induce transient pulp inflammation294445. Findings of the present work are in agreement with those studies.…”

Section: Discussionsupporting

confidence: 92%

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Caries-resistant bonding layer in dentin

Zhou¹,

Niu

et al. 2016

Sci Rep

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The present study examined the mechanism for caries resistance and the pulp responses in vital teeth following the use of the augmented-pressure adhesive displacement technique. Dentin adhesives were applied to the surface of sound dentin disks in 4 experimental groups: non-antibacterial adhesive and gentle adhesive displacement (N-G), non-antibacterial adhesive and augmented-pressure adhesive displacement (N-H), antibacterial adhesive and gentle adhesive displacement (A-G), antibacterial adhesive and augmented-pressure adhesive displacement (A-H). The depth of demineralization induced by biological or chemical demineralization models was measured using confocal laser scanning microscopy and analyzed with two-way ANOVA. Pulp responses of vital dog’s teeth to the augmented-pressure adhesive displacement technique were evaluated using light microscopy. Depth of demineralization was significantly affected by “adhesive type” and “intensity of adhesive displacement” for biological demineralization. For chemical demineralization, only “intensity of adhesive displacement” showed significant influence on lesion depth. Pulp response of 0.1, 0.2 and 0.3 MPa groups showed only moderate disorganization of the odontoblast layer at 24 hours that completely re-organized after 3 weeks. Augmented-pressure adhesive displacement improves the caries resistance property of bonded dentin and does not cause irreversible pulpal damage to vital teeth when the air pressure employed is equal or smaller than 0.3 MPa.

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Section: Discussionsupporting

confidence: 92%

“…The specimens were sectioned into 4 μm-thick slices. The slices were stained using hematoxylin-eosin and examined using light microscopy (BX-43, Olympus Corp., Tokyo, Japan)282930.…”

Section: Methodsmentioning

confidence: 99%

Caries-resistant bonding layer in dentin

Zhou¹,

Niu

et al. 2016

Sci Rep

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“…Furthermore, evidence of dentinal fluid flow after the application of adhesive suggests that the polymerized adhesive could not completely seal the deep vital dentin [56,57]. Unpolymerized monomers may be released out of the polymer matrix [58] and diffuse through the tubules into the pulp [59], causing damage to pulpal tissues [60]. Therefore, with the release of unpolymerized monomer, the irritation to pulp could persist and cause chronic inflammatory response, as observed in the 30 d groups.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of antibacterial and remineralizing nanocomposite and adhesive in rat tooth cavity model

Wang

Weir

et al. 2014

Acta Biomaterialia

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Antibacterial and remineralizing dental composites and adhesives were recently developed to inhibit biofilm acids and combat secondary caries. It is not clear what effect these materials will have on dental pulps in vivo. The objectives of this study were to investigate the antibacterial and remineralizing restorations in a rat tooth cavity model, and determine pulpal inflammatory response and tertiary dentin formation. Nanoparticles of amorphous calcium phosphate (NACP) and antibacterial dimethylaminododecyl methacrylate (DMADDM) were synthesized and incorporated into a composite and an adhesive. Occlusal cavities were prepared in the first molars of rats and restored with four types of restoration: Control composite and adhesive; control plus DMADDM; control plus NACP; and control plus both DMADDM and NACP. At 8 or 30 days (d), rat molars were harvested for histological analysis. For inflammatory cell response, regardless of time periods, NACP group and DMADDM+NACP group showed lower scores (better biocompatibility) than control group (p = 0.014 for 8 d, p = 0.018 for 30 d). For tissue disorganization, NACP and DMADDM+NACP had better scores than control (p = 0.027) at 30 d. At 8 d, restorations containing NACP had tertiary dentin thickness (TDT) that was 5-6 fold that of control. At 30 d, restorations containing NACP had TDT that was 4-6 fold that of control. In conclusion, novel antibacterial and remineralizing restorations were tested in rat teeth in vivo for the first time. Composite and adhesive containing NACP and DMADDM exhibited milder pulpal inflammation and much greater tertiary dentin formation, than control adhesive and composite. Therefore, the novel composite and adhesive containing NACP and DMADDM are promising as a new therapeutic restorative system to not only combat oral pathogens and biofilm acids as shown previously, but also facilitate the healing of the dentin-pulp complex.

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“…This is a result of interaction of resin and its components with plasma proteins, tissues, and connective tissue cells [100,101]. In the following studies, it has been shown that non-polymerized resin compounds released from dental adhesives cause chronic pulp inlammatory response [102]. The response of the pulp to the dental materials could be modiied by bacteria's and their products, and also afect by carious dentine, cavity preparation, and bleeding.…”

Section: In Vivo Biocompatibility Testings Of Dental Adhesivesmentioning

confidence: 99%

Biocompatibility of Dental Adhesives

Tadin¹,

Gavić²,

Galić³

2016

Adhesives - Applications and Properties

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The accomplishment of developing a truly adhesive bond between a restorative material and the natural tooth structures is the goal of adhesive dentistry. Dentine adhesive systems come into close contact with dental and oral tissue, especially the pulp and gingival cells. Due to this close and long-term contact, adhesives should exhibit a high degree of biocompatibility. "iocompatibility is one of the most important properties of dental materials, and adhesives are no exception. It has been long demonstrated that diferent components of adhesives can be released. Numerous in vitro investigations have shown that released monomers and other components can cause damage to cultured cells. In addition, many in vivo studies have shown that uncured components which reach the pulpal space cause inlammatory response and tissue disorganization. Only a combination of various in vitro and in vivo tests can provide an overview of the interaction of biomaterials with the host. Therefore, it is necessary on a regular basis to carry out and re-verify the biological compatibility of the increasing number of new dental materials. "dhesives should be biofunctional, protective, and preventive, with health-promoting efects that contribute to a beter prognosis for restorative treatments and its biocompatibility.

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Response of human pulps following acid conditioning and application of a bonding agent in deep cavities

Cited by 65 publications

References 28 publications

Caries-resistant bonding layer in dentin

Caries-resistant bonding layer in dentin

Evaluation of antibacterial and remineralizing nanocomposite and adhesive in rat tooth cavity model

Biocompatibility of Dental Adhesives

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