Response of human normal and leukemia cells to factors released by amnion fragments in vitro

Abstract

Amnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on migration and proliferation of human umbilical vein endothelial primary cells (HUVECs), freshly isolated bone marrow mononuclear cells (BM MNCs), and Jurkat leukemia cell line. Amnion membrane was freshly isolated from healthy placenta and its fragments cultured in vitro to produce hAM CCM. Members of the IGFBP protein family made up one third of all assayed proteins present in the hAM medium. The hAM CCM did not affect the proliferation rate of HUVECs or MNCs, but we observed more intensive migration of those cells, and lower expression of CD31 surface antigen on HUVECs as compared to control cultures. In contrast, Jurkat cells did not respond to hAM CCM treatment by proliferation or mobility change. The conditioned medium from 24-h cultures of human amnion is easy to obtain and is a convenient source of various growth and other factors that may be useful in practical medicine.

Prolactin modifies the in vitro LPS‐induced chemotactic capabilities in human fetal membranes at the term of gestation

“…Unlike amnion, the choriodecidua is in direct contact with a greater number of maternal immune cells throughout the pregnancy. In vitro evidence, has supported that amnion possesses intrinsic immunoregulatory properties, the co‐culture with amniotic cells or conditioned media from the amniotic cells inhibits the activation/proliferation of T cells, 40,41 and the infiltration and survival of neutrophils 42,43 …”

Methods and criteria for validating the multimodal functions of perinatal derivatives when used in oncological and antimicrobial applications

“…Alternative methods, such as Trypan blue analysis ( Chen et al, 2012 ) might be more difficult to reproduce and validate. Monitoring of electrical impedance changes ( Grzywocz et al, 2018 ) caused by the proliferation of HUVECs could be enabled by real-time systems. In addition, the digital endothelial tube formation assay-derived images could potentially enable the calculation of digital angiogenic indices ( Alshareeda et al, 2018 ), covering the numerical values of tube morphometry, such as extremities, number of segments, branches and the length of tubes.…”