Response of G protein-coupled receptor CED-1 in germline to polystyrene nanoparticles in<i>Caenorhabditis elegans</i>

Yang, Yunhan; Dong, Wenting; Wu, Qiuli; Wang, Dayong

doi:10.1039/d0na00867b

Cited by 28 publications

(4 citation statements)

References 63 publications

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“…However, the combined effects of microplastics and nanoplastics, especially at predicted environmental relevant concentration, on organisms remains unclear. Herein, locomotion behavior and brood size, as sensitive ecological indicators, could more effectively reflect the potential biotoxicity of environmental pollutants [ 43 , 44 ]. Firstly, we used sensitive endpoints (brood size and locomotion behavior) to examine the combinational effects between PS-50 and PS-500 in nematodes.…”

Section: Discussionmentioning

confidence: 99%

Combined Effects of Micro- and Nanoplastics at the Predicted Environmental Concentration on Functional State of Intestinal Barrier in Caenorhabditis elegans

Tan

Shi

et al. 2023

Toxics

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The possible toxicity caused by nanoplastics or microplastics on organisms has been extensively studied. However, the unavoidably combined effects of nanoplastics and microplastics on organisms, particularly intestinal toxicity, are rarely clear. Here, we employed Caenorhabditis elegans to investigate the combined effects of PS-50 (50 nm nanopolystyrene) and PS-500 (500 nm micropolystyrene) at environmentally relevant concentrations on the functional state of the intestinal barrier. Environmentally, after long-term treatment (4.5 days), coexposure to PS-50 (10 and 15 μg/L) and PS-500 (1 μg/L) resulted in more severe formation of toxicity in decreasing locomotion behavior, in inhibiting brood size, in inducing intestinal ROS production, and in inducting intestinal autofluorescence production, compared with single-exposure to PS-50 (10 and 15 μg/L) or PS-500 (1 μg/L). Additionally, coexposure to PS-50 (15 μg/L) and PS-500 (1 μg/L) remarkably caused an enhancement in intestinal permeability, but no detectable abnormality of intestinal morphology was observed in wild-type nematodes. Lastly, the downregulation of acs-22 or erm-1 expression and the upregulation expressions of genes required for controlling oxidative stress (sod-2, sod-3, isp-1, clk-1, gas-1, and ctl-3) served as a molecular basis to strongly explain the formation of intestinal toxicity caused by coexposure to PS-50 (15 μg/L) and PS-500 (1 μg/L). Our results suggested that combined exposure to microplastics and nanoplastics at the predicted environmental concentration causes intestinal toxicity by affecting the functional state of the intestinal barrier in organisms.

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Section: Discussionmentioning

confidence: 99%

Combined Effects of Micro- and Nanoplastics at the Predicted Environmental Concentration on Functional State of Intestinal Barrier in Caenorhabditis elegans

Tan

Shi

et al. 2023

Toxics

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“…ROS reflect levels of oxidative stress and are an important contributor to aging and disease [ 39 ]. ROS production in nematodes was measured using the 5′,6′-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H 2 DCFDA) fluorescent probe method [ 40 ]. The exposed nematodes were collected after washing 3 times with K-medium, and then placed in NGM plates containing 1 mL of CM-H2DCFDA (50 µM), fed OP50 and incubated for 2 h in an incubator at 20 °C.…”

Section: Methodsmentioning

confidence: 99%

Chemical Composition and Transgenerational Effects on Caenorhabditis elegans of Seasonal Fine Particulate Matter

Yang

Liu

et al. 2023

Toxics

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While numerous studies have demonstrated the adverse effects of fine particulate matter (PM) on human health, little attention has been paid to its impact on offspring health. The multigenerational toxic effects on Caenorhabditis elegans (C. elegans) were investigated by acute exposure. PM2.5 and PM1 samples were collected and analysed for their chemical composition (inorganic ions, metals, OM, PAHs) in different seasons from April 2019 to January 2020 in Lin’an, China. A higher proportion of organic carbon components (34.3%, 35.9%) and PAHs (0.0144%, 0.0200%) occupied the PM2.5 and PM1 samples in winter, respectively. PM1 in summer was enriched with some metal elements (2.7%). Exposure to fine PM caused developmental slowing and increased germ cell apoptosis, as well as inducing intestinal autofluorescence and reactive oxygen species (ROS) production. PM1 caused stronger toxic effects than PM2.5. The correlation between PM component and F0 generation toxicity index was analysed. Body length, germ cell apoptosis and intestinal autofluorescence were all highly correlated with Cu, As, Pb, OC and PAHs, most strongly with PAHs. The highest correlation coefficients between ROS and each component are SO42- (R = 0.743), Cd (R = 0.816) and OC (R = 0.716). The results imply that OC, PAHs and some transition metals play an important role in the toxicity of fine PM to C. elegans, where the organic fraction may be the key toxicogenic component. The multigenerational studies show that PM toxicity can be passed from parent to offspring, and gradually returns to control levels in the F3–F4 generation with germ cell apoptosis being restored in the F4 generation. Therefore, the adverse effects of PM on reproductive damage are more profound.

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“…G protein-coupled receptors (GPCRs) constitute the largest family of transmembrane ™ receptors, playing a crucial role in various physiological processes [18]. Prior research has demonstrated the involvement of GPCRs in innate/adaptive immunity, environmental signal detection, and pathogen defense in the model nematode Caenorhabditis elegans [19][20][21]. The prefix "sr" designates over 1300 potential chemoreceptor genes identified in C. elegans, which belong to the receptor superfamilies Sra (Sra, Srb, Srab, Sre) and Str (Srh, Str, Sri, Srd, Srj, Srm, Srn), and Srg (Srx, Srt, Srg, Sru, Srv, Srxa) [22].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Functional Analysis of GPCR Gene Bx-srh-1 in Pinewood Nematode (Bursaphelenchus xylophilus)

Cao

Wang

et al. 2023

Forests

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Bursaphelenchus xylophilus, also known as the pine wood nematode (PWD), which causes pine wilt disease (PWD), is one of the most devastating diseases affecting pine forests globally. G protein-coupled receptors (GPCRs) are crucial in many biological processes and serve as privileged points of communication between cells and the surrounding environment. α-pinene has been found to play a crucial role in combating the infection, colonization, and early stages of pathogenesis caused by B. xylophilus. In this study, we investigated the molecular characteristics and biological functions of the GPCR gene Bx-srh-1 in B. xylophilus. Fluorescence in situ hybridization (FISH) was performed to determine the spatial expression patterns of Bx-srh-1 in B. xylophilus. The results indicated that Bx-srh-1 is expressed in the intestine and subcutaneous tissues of J2 and J3 juveniles and in the spicules of adult males and vulvae of adult females. RNA interference (RNAi) was used to analyze Bx-srh-1 gene function, and we examined the expression patterns of Bx-srh-1 in B. xylophilus under α-pinene stress. The RNA interference indicated that Bx-srh-1 was involved in the reproductive ability and pathogenicity of B. xylophilus; the expression levels of Bx-srh-1 significantly increased after the exposure to α-pinene for 12 h, and they peaked at 48 h. Silencing Bx-srh-1 may therefore lead to a reduction in B. xylophilus reproduction and pathogenicity. These results demonstrate that Bx-srh-1 is related to the feeding behavior, reproduction, pathogenicity, and resistance to α-pinene process of B. xylophilus.

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Response of G protein-coupled receptor CED-1 in germline to polystyrene nanoparticles inCaenorhabditis elegans

Abstract: The deposition of certain amount of nanopolystyrene (NPS) could be observed in gonad of Caenorhabditis elegans. However, we still know little about the response of germline to NPS exposure. In...

Cited by 28 publications

References 63 publications

Combined Effects of Micro- and Nanoplastics at the Predicted Environmental Concentration on Functional State of Intestinal Barrier in Caenorhabditis elegans

Combined Effects of Micro- and Nanoplastics at the Predicted Environmental Concentration on Functional State of Intestinal Barrier in Caenorhabditis elegans

Chemical Composition and Transgenerational Effects on Caenorhabditis elegans of Seasonal Fine Particulate Matter

Molecular Characterization and Functional Analysis of GPCR Gene Bx-srh-1 in Pinewood Nematode (Bursaphelenchus xylophilus)

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