Response of Dental Pulp Stem Cells to Synthetic, Allograft, and Xenograft Bone Scaffolds

Motamedian, Saeed Reza; Tabatabaei, Fahimeh; Akhlaghi, Fahimeh; Torshabi, Maryam; Gholamin, Parisan; Khojasteh, Arash

doi:10.11607/prd.2121

Cited by 25 publications

(42 citation statements)

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“…Using MSCs with the same technique of harvesting and delivery, when cultured over biphasic HA/TCP and mixed with the platelet rich in growth factor as an adhesive agent, resulted in increased bone formation of up to 50% [ 6 ]. It is assumed that synthetic or allogenic scaffolds with a structure similar to natural bone may be appropriate replacements for natural human bone and the literature suggests that freeze dried allografts are more osteoinductive than β -TCP [ 48 ]. In addition NBBM's biocompatibility as a cell carrier was further evaluated in this study.…”

Section: Discussionmentioning

confidence: 99%

Lateral Ramus Cortical Bone Plate in Alveolar Cleft Osteoplasty with Concomitant Use of Buccal Fat Pad Derived Cells and Autogenous Bone: Phase I Clinical Trial

Khojasteh

Kheiri

Behnia

et al. 2017

BioMed Research International

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Tissue regeneration has become a promising treatment for craniomaxillofacial bone defects such as alveolar clefts. This study sought to assess the efficacy of lateral ramus cortical plate with buccal fat pad derived mesenchymal stem cells (BFSCs) in treatment of human alveolar cleft defects. Ten patients with unilateral anterior maxillary cleft met the inclusion criteria and were assigned to three treatment groups. First group was treated with anterior iliac crest (AIC) bone and a collagen membrane (AIC group), the second group was treated with lateral ramus cortical bone plate (LRCP) with BFSCs mounted on a natural bovine bone mineral (LRCP+BFSC), and the third group was treated with AIC bone, BFSCs cultured on natural bovine bone mineral, and a collagen membrane (AIC+BFSC). The amount of regenerated bone was measured using cone beam computed tomography 6 months postoperatively. AIC group showed the least amount of new bone formation (70 ± 10.40%). LRCP+BFSC group demonstrated defect closure and higher amounts of new bone formation (75 ± 3.5%) but less than AIC+BFSC (82.5 ± 6.45%), suggesting that use of BFSCs within LRCP cage and AIC may enhance bone regeneration in alveolar cleft bone defects; however, the differences were not statistically significant. This clinical trial was registered at clinicaltrial.gov with NCT02859025 identifier.

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Section: Discussionmentioning

confidence: 99%

Lateral Ramus Cortical Bone Plate in Alveolar Cleft Osteoplasty with Concomitant Use of Buccal Fat Pad Derived Cells and Autogenous Bone: Phase I Clinical Trial

Khojasteh

Kheiri

Behnia

et al. 2017

BioMed Research International

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“…However, cell attachment was more probable on rough edges of FDBA granules. A previous study also showed attachment of clusters of long cells penetrating into scaffold pores and attached to its rough edges and formation of collagen-like fibers on FDBA granules (Motamedian et al 2017 ). Similarly, (Tang et al 2002 ) showed penetration and attachment of mesenchymal stem cells (MSCs) to FDBA pores.…”

Section: Discussionmentioning

confidence: 81%

Allogenic vs. synthetic granules for bone tissue engineering: an in vitro study

et al. 2018

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The aim of this study was to compare human dental pulp stem cells’ (DPSCs) attachment, proliferation and osteogenic differentiation on allogenic and synthetic biphasic bone granules. In this in vitro study, two types of bone granules were used: allograft [freeze-dried bone allograft (FDBA)] and biphasic granules [hydroxyapatite/beta-tricalcium phosphate (HA/β-TCP)]. By isolation of DPSCs, their attachment to bone granules was observed by scanning electron microscope (SEM) at day 1 and 7 of cultivation. Vital cells were measured by MTT assay at 1, 3, and 7 days of cell culture. Comparison of vital cells at different time points was considered as cell proliferation. Finally, differentiation of DPSCs was evaluated by measurement of alkaline phosphatase (ALP) activity 3, 7, 14, and 21 days after cell seeding in standard and osteogenic media. Data were analyzed using two-way ANOVA with a significant level of 0.05. Attachment of DPSCs on FDBA granules seemed relatively stronger. The number of cells (based on MTT values) and ALP activity of the cells cultured on both study groups increased between time points (p ≤ 0.001). FDBA granules had more cells compared to HA/β-TCP granules (p < 0.001). There was no significant difference between ALP activity of two study groups cultured in the standard medium (p = 0.347) and they were both higher than the control group (p < 0.05). In the osteogenic medium, FDBA group had significantly higher ALP activity compared to HA/β-TCP (p = 0.035) and control (p = 0.001) groups while there was no significant difference between ALP activity of HA/β-TCP and control groups (p = 0.645). In conclusion, current in vitro study revealed that FDBA granules have more potential in supporting DPSCs attachment and proliferation and inducing their ALP activity compared to HA/β-TCP granules. Therefore, FDBA could serve as a proper bone substitute material.

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“…The stem cells responded differently to various types of scaffolds (Motamedian et al, 2016). Eluted zinc released from zinc-modified titanium which is frequently applied in dental and maxillofacial implantation could stimulate osteoblast differentiation of DPSCs (Yusa et al, 2016a,b).…”

Section: Applications Of Somatic Stem Cells In Oral and Maxillofacialmentioning

confidence: 99%

Application of Stem Cells in Oral Disease Therapy: Progresses and Perspectives

Yang¹,

Qiu²,

Zhou³

et al. 2017

Front. Physiol.

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Stem cells are undifferentiated and pluripotent cells that can differentiate into specialized cells with a more specific function. Stem cell therapies become preferred methods for the treatment of multiple diseases. Oral and maxillofacial defect is one kind of the diseases that could be most possibly cured by stem cell therapies. Here we discussed oral diseases, oral adult stem cells, iPS cells, and the progresses/challenges/perspectives of application of stem cells for oral disease treatment.

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Response of Dental Pulp Stem Cells to Synthetic, Allograft, and Xenograft Bone Scaffolds

Cited by 25 publications

References 0 publications

Lateral Ramus Cortical Bone Plate in Alveolar Cleft Osteoplasty with Concomitant Use of Buccal Fat Pad Derived Cells and Autogenous Bone: Phase I Clinical Trial

Lateral Ramus Cortical Bone Plate in Alveolar Cleft Osteoplasty with Concomitant Use of Buccal Fat Pad Derived Cells and Autogenous Bone: Phase I Clinical Trial

Allogenic vs. synthetic granules for bone tissue engineering: an in vitro study

Application of Stem Cells in Oral Disease Therapy: Progresses and Perspectives

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