Response of catalase to drought in barley (Hordeum vulgare L.) seedlings and its purification

Abstract

Drought induced oxidative stress triggers anti-oxidative system in cell to minimize oxidative damage where catalase plays vital role to neutralize H 2 O 2. In this work, catalase activity was evaluated to examine the role of catalase in barley (Hordeum vulgare L.) seedlings under drought stress. As compared to control, catalase activity increased with decreasing soil moisture where 219% higher activity were recorded at 10% soil moisture of field capacity (FC) compared to control (75% FC), but was reduced below 10% FC. Four different catalase isozymes that specifically accumulated in barley leaves in response to drought (10% FC) which nominated catalase, particularly CAT4 and CAT2, as key players for H 2 O 2 scavenging were identified. However, for future study, one catalase was purified from barley leaves with an apparent molecular weight of 54 kDA and specific activity of 871.32 µmol min-1 mg-1 protein. Therefore, in this study, it was found that four CAT isozymes in barley leaf under drought, and the purified catalase needs characterization at molecular level for further biotechnical use.

Identification and Expression Profiling of Two Saudi Arabia Catalase Genes from Wheat and Barley in Response to Abiotic and Hormonal Stresses

“…Recently, four different catalase genes were identified from barley. CAT2 and CAT4 were activated in response to drought stress [ 15 ]. Catalase genes are involved in plants’ response to both abiotic and biotic stress [ 6 , 10 , 16 ].…”

An Insight to the Various Applications of Hydroxyapatite

“…Holková et al [71] purified lipoxygenases (LOX) from opium poppy cultures (Papaver somniferum L.) via a two-step chromatography process. LOX underwent ammonium sulfate precipitation before chromatography with HAp sorbent to be purified from Papaver somniferum L.. Rohman et al [72] purified catalase from the leaves of barley (Hordeum vulgare L.) to examine the role of catalase in Hordeum vulgare L. under saline stress. Proteins from the leaves of Hordeum vulgare L. were extracted in Tris-HCl buffer before a 3-step chromatography process involving HAp chromatography to obtain catalase.…”