This work aims is to investigate the structural dynamic response of steel-concrete composite floors, when subjected to rhythmic human activities, based on experimental tests and numerical modelling, from the point of view of human comfort. The main focus of this paper is to study the effect of the dynamic interaction between the occupants and the floors, representing these people by SDOF biodynamic models (mass-spring-damper systems), in order to better evaluate the dynamic response and the vibration serviceability states. This way, the investigated structural model is related to a steel-concrete composite building which is composed of three floors used for aerobics classes, with dimensions of 20m x 20m, a total area of 1200m² (3 x 400m²) and a ceiling height of 4m. The numerical modelling of the structure was performed by ANSYS program and was based on the finite element method (FEM). In order to evaluate the dynamic response of the investigated steelconcrete composite building, accelerations associated with 32 individuals practicing rhythmic activities were experimentally obtained to introduce them into the finite element model. Thus, the dynamic response of the building floors was evaluated in terms of the peak accelerations, RMS and VDV values, according to human comfort criteria and considering situations of the design practice.