Response mechanism of mine-isolated fungus <i>Aspergillus niger</i> IOC 4687 to copper stress determined by proteomics

Dias, Mirella; Lacerda, José Thalles Jocelino Gomes de; Almeida, Silas Perdigão Cota de; Andrade, Lidiane Maria de; Nascimento, Cláudio Augusto Oller do; Rozas, Enrique E.; Mendes, Maria Anita

doi:10.1039/c9mt00137a

Cited by 9 publications

(4 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the emergence of a lag phase may be due to oxidative stress induced by copper requiring the consumption of glutathione (GSH), whose synthesis requires additional energy. A similar adaptation mechanism has been observed in Aspergillus niger [51].…”

Section: Heavy Metals Resistancesupporting

confidence: 77%

Biochemical and Metabolomic Responses of Antarctic Bacterium Planococcus sp. O5 Induced by Copper Ion

Cheng

Shi

Wei

et al. 2022

Toxics

View full text Add to dashboard Cite

Heavy metal pollution in the Antarctic has gone beyond our imagination. Copper toxicity is a selective pressure on Planococcus sp. O5. We observed relatively broad tolerance in the polar bacterium. The heavy metal resistance pattern is Pb2+ > Cu2+ > Cd2+ > Hg2+ > Zn2+. In the study, we combined biochemical and metabolomics approaches to investigate the Cu2+ adaptation mechanisms of the Antarctic bacterium. Biochemical analysis revealed that copper treatment elevated the activity of antioxidants and enzymes, maintaining the bacterial redox state balance and normal cell division and growth. Metabolomics analysis demonstrated that fatty acids, amino acids, and carbohydrates played dominant roles in copper stress adaptation. The findings suggested that the adaptive mechanisms of strain O5 to copper stress included protein synthesis and repair, accumulation of organic permeable substances, up-regulation of energy metabolism, and the formation of fatty acids.

show abstract

Section: Heavy Metals Resistancesupporting

confidence: 77%

Biochemical and Metabolomic Responses of Antarctic Bacterium Planococcus sp. O5 Induced by Copper Ion

Cheng

Shi

Wei

et al. 2022

Toxics

View full text Add to dashboard Cite

show abstract

“…The highly upregulated protein hydroxymethylglutaryl-CoA synthase with 5.19-fold upregulation is responsible for the production of secondary metabolite carotenoid from the precursor molecule of acetyl-CoA when stimulated by the heavy metal stress ( Bhosale, 2004 ). The protein serine/threonine phosphatase with 3.42-fold upregulation is responsible for maintaining the conformation of cell organelles and proteasomes ( Dias et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…The important hub proteins expressed were cytochrome-c oxidase that is mitochondrial proteins and catalyst in the electron transport chain and is responsible for the transport of heavy metals particularly copper ( Dias et al, 2019 ). The second cluster (blue) had hub protein ATP synthase.…”

Section: Discussionmentioning

confidence: 99%

Insight Into the Molecular Mechanisms Underpinning the Mycoremediation of Multiple Metals by Proteomic Technique

et al. 2022

View full text Add to dashboard Cite

We investigated the fungus Aspergillus fumigatus PD-18 responses when subjected to the multimetal combination (Total Cr, Cd2+, Cu2+, Ni2+, Pb2+, and Zn2+) in synthetic composite media. To understand how multimetal stress impacts fungal cells at the molecular level, the cellular response of A. fumigatus PD-18 to 30 mg/L multimetal stress (5 mg/L of each heavy metal) was determined by proteomics. The comparative fungal proteomics displayed the remarkable inherent intracellular and extracellular mechanism of metal resistance and tolerance potential of A. fumigatus PD-18. This study reported 2,238 proteins of which 434 proteins were exclusively expressed in multimetal extracts. The most predominant functional class expressed was for cellular processing and signaling. The type of proteins and the number of proteins that were upregulated due to various stress tolerance mechanisms were post-translational modification, protein turnover, and chaperones (42); translation, ribosomal structure, and biogenesis (60); and intracellular trafficking, secretion, and vesicular transport (18). In addition, free radical scavenging antioxidant proteins, such as superoxide dismutase, were upregulated upto 3.45-fold and transporter systems, such as protein transport (SEC31), upto 3.31-fold to combat the oxidative stress caused by the multiple metals. Also, protein–protein interaction network analysis revealed that cytochrome c oxidase and 60S ribosomal protein played key roles to detoxify the multimetal. To the best of our knowledge, this study of A. fumigatus PD-18 provides valuable insights toward the growing research in comprehending the metal microbe interactions in the presence of multimetal. This will facilitate in development of novel molecular markers for contaminant bioremediation.

show abstract

“…Price et al (2001) found that A. niger is capable of removing 91% of copper and 70% of zinc from swine wastewater. Proteomic analysis of A. niger showed that its capacity to absorb metals was boosted by physiological modification under stress, more specifically antioxidant responses induced by activity of catalase, superoxide dismutase, and cytochrome c peroxidase (Dias et al, 2019). Luna et al (2015) found that exposing A. niger cells to increasingly large concentrations of copper led to ultrastructural changes in the cell surface, electron density, thickness, and septation.…”

Section: Discussionmentioning

confidence: 99%

Biosorption of Copper in Swine Manure Using Aspergillus and Yeast: Characterization and Its Microbial Diversity Study

Zhen

Wang

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Dietary copper supplementation in the feed of piglets generally exceeds 250–800 mg/kg, where a higher quantity (>250 mg/kg) can promote growth and improve feed conversion. Despite the reported positive effects, 90% of copper is excreted and can accumulate and pollute the soil. Data indicate that fungi have a biosorptive capacity for copper. Thus, the objectives of the present experiment were to study the effects of adding different strains of fungi on the biosorptive capacity for copper in swine manure and to evaluate potential effects on microbiota profiles. Aspergillus niger (AN), Aspergillus oryzae (AO), and Saccharomyces cerevisiae (SC) were selected, and each added 0.4% into swine manure, which contain 250 mg/kg of copper. The incubations lasted for 29 days, and biosorption parameters were analyzed on the 8th (D8), 15th (D15), 22nd (D22), and 29th (D29) day. Results showed that after biosorption, temperature was 18.47–18.77°C; pH was 6.33–6.91; and content of aflatoxin B1, ochratoxin A, and deoxynivalenol were low. In addition, residual copper concentration with AN was the lowest on D15, D22, and D29. The copper biosorption rate was also highest with AN, averaging 84.85% on D29. Biosorption values for AO reached 81.12% and for SC were lower than 80%. Illumina sequencing of 16S and ITS rRNA gene revealed that fungal treatments reduced the diversity and richness of fungal abundance, but had no effect on bacterial abundance. Unknown_Marinilabiliaceae, Proteiniphilum, Tissierella, and Curvibacter were the dominant bacteria, while Aspergillus and Trichoderma were the dominant fungi. However, the added strain of S. cerevisiae was observed to be lower than the dominant fungi, which contained less than 0.05%. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment predicted via PICRUSt2 that there were bacterial genes potentially related to various aspects of metabolism and environmental information processing. Overall, data indicated that Aspergillus can provide microbial materials for adsorption of copper.

show abstract

Response mechanism of mine-isolated fungus Aspergillus niger IOC 4687 to copper stress determined by proteomics

Abstract: Proteomic analysis of the fungus Aspergillus niger showed that its capacity to absorb metals was boosted by physiological modification under metal stress conditions.

Cited by 9 publications

References 43 publications

Biochemical and Metabolomic Responses of Antarctic Bacterium Planococcus sp. O5 Induced by Copper Ion

Biochemical and Metabolomic Responses of Antarctic Bacterium Planococcus sp. O5 Induced by Copper Ion

Insight Into the Molecular Mechanisms Underpinning the Mycoremediation of Multiple Metals by Proteomic Technique

Biosorption of Copper in Swine Manure Using Aspergillus and Yeast: Characterization and Its Microbial Diversity Study

Contact Info

Product

Resources

About