Response and Recovery of Brain Acetylcholinesterase Activity in the European Eel,Anguilla anguilla,Exposed to Fenitrothion

Sancho, E.; Ferrando, M.D.; Andreu, E.

doi:10.1006/eesa.1997.1579

Cited by 55 publications

(31 citation statements)

References 25 publications

(32 reference statements)

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“…5 as well. It could be found that after additional 8 days of elimination, the ChE activities in liver and brain kept on reducing, but those in gill and muscle increased slightly, which confirmed studies by other researchers (Straus and Chambers, 1995;Sancho et al, 1997). Roex et al (2003) reported that AChE inhibition in zebrafish after chronic exposure to parathion was correlated with exposure concentration, but not with exposure time.…”

Section: Cholinesterase Activity Assaysupporting

confidence: 85%

“…In the present work, recovery of ChE activity also was observed in gill and muscle of medaka after 8 days of recovery period. This recovery is due to renewed synthesis of AChE in fish body (Sancho et al, 1997). Essentially all chemicals reaction in cells are catalyzed by enzymes, thus the toxic action of an exogenous chemical in the cell almost always involves disturbances in enzyme function.…”

Section: Cholinesterase Activity Assaymentioning

confidence: 99%

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Methylmercury accumulation, histopathology effects, and cholinesterase activity alterations in medaka (Oryzias latipes) following sublethal exposure to methylmercury chloride

Liao

Shi

et al. 2006

Environmental Toxicology and Pharmacology

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Section: Cholinesterase Activity Assaysupporting

confidence: 85%

Section: Cholinesterase Activity Assaymentioning

confidence: 99%

Methylmercury accumulation, histopathology effects, and cholinesterase activity alterations in medaka (Oryzias latipes) following sublethal exposure to methylmercury chloride

Liao

Shi

et al. 2006

Environmental Toxicology and Pharmacology

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“…As the recovery of enzyme activity is basically a result of the de novo synthesis of enzyme protein, the greater the degree of inhibition, the more protein synthesis is required (Fulton & Key 2001). Sancho et al (1997) found that the AChE inhibition of fenitrothionintoxicated fish decreased from 64 to 49% and 37% after 96 and 192 h, respectively, of recovery in clean water. In the current study, brain AChE was still inhibited by 40% after 96 h of recovery in clean water, but only by 26% in the 0.5 mM NAC solution.…”

Section: Discussionmentioning

confidence: 94%

“…In the present study, brain AChE inhibition in dichlorvos-exposed fish increased over time and reached 80% of the control activity after 96 h exposure, but no mortality was experienced. Sancho et al (1997) found a 64% inhibition of brain AChE in European eels exposed for 96 h to a sublethal (20% of the corresponding 96 h LC 50 ) concentration of the OP insecticide fenitrothion, which is a less potent cholinesterase inhibitor than dichlorvos because it requires activation to the oxygen analogue fenitrooxon.…”

Section: Discussionmentioning

confidence: 98%

Increased recovery of brain acetylcholinesterase activity in dichlorvos-intoxicated European eels Anguilla anguilla by bath treatment with N-acetylcysteine

Peña-Llopis¹,

Ferrando²,

Jb³

2003

Dis. Aquat. Org.

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Organophosphate (OP) pesticides are widely used as antiparasitic chemicals in finfish aquaculture. However, current antidotes cannot be applied to treat intoxicated fish. We showed in previous studies the importance of glutathione (GSH) metabolism in pesticide resistance of the European eel Anguilla anguilla L. The present work studied the effects of the antioxidant and glutathione pro-drug N-acetyl-L-cysteine (NAC) on the recovery of European eels exposed for 96 h to a sublethal concentration (0.17 mg l -1 ; 20% of its 96 h LC 50 ) of the OP pesticide dichlorvos (2, 2-dichlorovinyl dimethyl phosphate; DDVP). This insecticide and acaricide decreased muscular GSH content and increased oxidised glutathione (GSSG), lowering the GSH:GSSG ratio, which is indicative of a condition of oxidative stress. Acetylcholinesterase (AChE) and glutathione reductase (GR) activities in the brain, which were biomarkers of neurotoxicity and oxidative stress, respectively, were also highly inhibited. Recovery in a 0.5 mM (81.6 mg l -1 ) NAC concentration ameliorated muscular GSH depletion, GSH:GSSG ratio, and the inhibition of brain AChE and GR activities. Hence, this is the first evidence of improved recovery of organophosphate-poisoned fish by bath treatments.

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“…Acetylcholinesterase (AChE) is a classic biomarker for the presence of sublethal concentrations of organophosphorous and carbamate compounds, which are widely used for pest control and can reach water streams through agricultural and urban releases (Viarengo et al, 2007). Several studies using fish species have shown inhibition of cholinesterase activity in the presence of organophosphate compounds in water (Sancho et al, 1997;Dutta and Arends, 2003). In addition to organophosphate and carbamates, other agricultural compounds such as glyphosate are shown to inhibit AchE in fish (Moraes et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Assessment of water pollution in the Brazilian Pampa biome by means of stress biomarkers in tadpoles of the leaf frog Phyllomedusa iheringii (Anura: Hylidae)

Santos

Melo

Silva

et al. 2016

Preprint

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The Brazilian Pampa biome is currently under constant threat due to increase of agriculture and improper management of urban effluents. Studies with focus on the assessment of impacts caused by human activities in this biome are scarce. In the present study we measured stress-related biomarkers in tadpoles of the leaf frog Phyllomedusa iheringii, an endemic species to the Pampa biome, and tested its suitability as a bioindicator for the assessment of potential aquatic contamination in selected ponds (S1 and S2) nearby agricultural areas in comparison to a reference site. A significant decrease in acetylcholinesterase activity was observed in S2 when compared to S1 and reference. The levels of total-hydroperoxides were increased in S2 site. In parallel, increased activity of the antioxidant enzymes catalase, superoxide dismutase and glutathione S-transferase were observed in S2 when compared to S1 and reference. Further studies are necessary in order to correlate the changes observed here with different chemical stressors in water, as well as to elucidate mechanisms of toxicity induced by pesticides in amphibian species endemic to the Pampa biome. Nevertheless, our study validates Phyllomedusa iheringii as a valuable bioindicator in environmental studies.

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Response and Recovery of Brain Acetylcholinesterase Activity in the European Eel,Anguilla anguilla,Exposed to Fenitrothion

Cited by 55 publications

References 25 publications

Methylmercury accumulation, histopathology effects, and cholinesterase activity alterations in medaka (Oryzias latipes) following sublethal exposure to methylmercury chloride

Methylmercury accumulation, histopathology effects, and cholinesterase activity alterations in medaka (Oryzias latipes) following sublethal exposure to methylmercury chloride

Increased recovery of brain acetylcholinesterase activity in dichlorvos-intoxicated European eels Anguilla anguilla by bath treatment with N-acetylcysteine

Assessment of water pollution in the Brazilian Pampa biome by means of stress biomarkers in tadpoles of the leaf frog Phyllomedusa iheringii (Anura: Hylidae)

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