Respiratory quinolones can eradicate amoxicillin-induced mature biofilms and nontypeable Haemophilus influenzae in biofilms

Umar, Nafisa K.; Kono, Masamitsu; Sakatani, Hideki; Murakami, Daichi; Onishi, Yuki; Kamiyama, Tsutomu; Iyo, Takuro; Hijiya, Masayoshi; Shiga, Tatsuya; Kinoshita, Tetsuya; Tamagawa, Shunji; Hiraoka, Masanobu; Ohtani, Makiko; Hotomi, Muneki

doi:10.1016/j.jiac.2022.07.019

Cited by 3 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have found that high concentrations of amoxicillin cannot remove NTHi from biofilms, while respiratory quinolones can inhibit biofilm formation and eradicate mature biofilms and can kill NTHi in biofilms even under sub-MIC conditions. 114 Simultaneous administration of carbapenem, neoquinolone and macrolide has the effect of anti NTHi mature biofilm. 115 The combination of metal chelating agent EDTA and antibiotics can inhibit biofilm formation and damage mature biofilms, and increase the sensitivity of NTHi biofilms to ampicillin and ciprofloxacin.…”

Section: H Influenzae Biofilmmentioning

confidence: 99%

Epidemic Trends and Biofilm Formation Mechanisms of Haemophilus influenzae: Insights into Clinical Implications and Prevention Strategies

Xiao¹,

Su²,

Huang³

et al. 2023

IDR

View full text Add to dashboard Cite

Haemophilus influenzae (H. influenzae) is a significant pathogen responsible for causing respiratory tract infections and invasive diseases, leading to a considerable disease burden. The Haemophilus influenzae type b (Hib) conjugate vaccine has notably decreased the incidence of severe infections caused by Hib strains, and other non-typable H. influenzae (NTHi) serotypes have emerged as epidemic strains worldwide. As a result, the global epidemic trends and antibiotic resistance characteristics of H. influenzae have been altered. Researches on the virulence factors of H. influenzae, particularly the mechanisms underlying biofilm formation, and the development of anti-biofilm strategies hold significant clinical value. This article provides a summary of the epidemic trends, typing methods, virulence factors, biofilm formation mechanisms, and prevention strategies of H. influenzae. The increasing prevalence of NTHi strains and antibiotic resistance among H. influenzae, especially the high β-lactamase positivity and the emergence of BLNAR strains have increased clinical difficulties. Understanding its virulence factors, especially the formation mechanism of biofilm, and formulating effective anti-biofilm strategies may help to reduce the clinical impact. Therefore, future research efforts should focus on developing new approaches to prevent and control H. influenzae infections.

show abstract

Section: H Influenzae Biofilmmentioning

confidence: 99%

Epidemic Trends and Biofilm Formation Mechanisms of Haemophilus influenzae: Insights into Clinical Implications and Prevention Strategies

Xiao¹,

Su²,

Huang³

et al. 2023

IDR

View full text Add to dashboard Cite

show abstract

“… 2 , 15 , 18 Notably, NTHi biofilm formation is promoted by subinhibitory concentrations of β-lactam antibiotics. 19 , 20 Whereas biofilm protects NTHi against β-lactams and other antibiotics, 18 , 20–22 ECM destabilizers (e.g. EDTA and DNase) can restore the efficacy of antibiotics against NTHi in biofilm in vitro .…”

Section: Introductionmentioning

confidence: 99%

The alginate polymer OligoG alters susceptibility of biofilm-embedded non-typeableHaemophilus influenzaeto ampicillin and ciprofloxacin

Marienborg

Ambur

Økstad

et al. 2023

JAC-Antimicrobial Resistance

View full text Add to dashboard Cite

Objectives Treatment of respiratory infections with non-typeable Haemophilus influenzae (NTHi) in COPD patients is complicated by biofilm formation, protecting the bacteria against the hosts’ immune response and antibiotics. We investigated the antibiofilm and antibacterial effects of the alginate polymer OligoG, alone or combined with ampicillin or ciprofloxacin, on mature NTHi biofilms. Materials and methods Two unrelated COPD strains with PBP3-mediated β-lactam resistance, with additional TEM-1 β-lactamase (Hi-022) or quinolone resistance due to altered GyrA and ParC (Hi-072) were used. Antibiofilm and antibacterial effects were assessed macroscopically, by measurement of biofilm biomass (OD), and by viable cell counts, with determination of minimum biofilm inhibitory concentration (MBIC) and the novel parameter ‘minimum concentration for 2 log10 drop in viable cells in biofilm’ (MB2LDC). Drug interactions between OligoG and antibiotics were assessed by comparing expected and observed inhibitory effects (percent inhibition of no-treatment control) of combined treatment. Results OligoG had dose-dependent biofilm disruptive abilities and a weak inhibitory effect on viable cells. Combination with OligoG (64 g/L) significantly lowered MBIC for ampicillin (both strains) and MB2LDC for ciprofloxacin (Hi-022). For Hi-022, there was significant synergism between OligoG and both antibiotics. For Hi-072, interactions were subtle, but a tendency in direction of antagonism was significant at two concentrations of ciprofloxacin. Conclusions OligoG shows promise as a potential adjuvant to antibiotics in NTHi infections, but strain-specific factors appear to affect drug interactions and may lead to antagonism. More research is needed to clarify the mechanisms of action of OligoG and interactions with antibiotics.

show abstract

Molecular characteristics and biofilm formation capacity of nontypeable Haemophilus influenza strains isolated from lower respiratory tract in children

Xiao,

Su,

Chen

et al. 2024

Microbial Pathogenesis

View full text Add to dashboard Cite

Respiratory quinolones can eradicate amoxicillin-induced mature biofilms and nontypeable Haemophilus influenzae in biofilms

Cited by 3 publications

References 31 publications

Epidemic Trends and Biofilm Formation Mechanisms of Haemophilus influenzae: Insights into Clinical Implications and Prevention Strategies

Epidemic Trends and Biofilm Formation Mechanisms of Haemophilus influenzae: Insights into Clinical Implications and Prevention Strategies

The alginate polymer OligoG alters susceptibility of biofilm-embedded non-typeableHaemophilus influenzaeto ampicillin and ciprofloxacin

Molecular characteristics and biofilm formation capacity of nontypeable Haemophilus influenza strains isolated from lower respiratory tract in children

Contact Info

Product

Resources

About