Respiratory nitrate reduction by Desulfovibrio sp.

McCready, R. G. L.; Gould, W. Douglas; Cook, F. D.

doi:10.1007/bf00414476

Cited by 65 publications

(41 citation statements)

References 22 publications

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“…Control experiments showed that growth was not inhibited by higher concentrations of sulfide generated by sulfate-sufficient cultures. These results confirmed previous reports that sulfide produced during sulfate reduction inhibits, represses, or both inhibits and represses nitrate reduction (7,16).…”

Section: Resultssupporting

confidence: 93%

“…There are many examples in the literature of sulfate-reducing bacteria that are also able to reduce nitrate (14,16,17,27,31,38), but this capacity is absent from the two strains of Desulfovibrio vulgaris for which complete genome sequence data are available (12). There is little agreement concerning how nitrate reduction is regulated, even in strains of D. desulfuricans.…”

mentioning

confidence: 99%

“…However, nitrate reduction was prevented at sulfate concentrations typically used in media to propagate sulfate-reducing bacteria. In contrast, McCready et al (16) proposed that nitrate reduction is inhibited rather than genetically repressed by sulfide generated as a product of sulfate reduction, a proposal that has since been confirmed (7). At very low concentrations, sulfate was immediately consumed, so nitrate reduction was not inhibited.…”

mentioning

confidence: 99%

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Preferential Reduction of the Thermodynamically Less Favorable Electron Acceptor, Sulfate, by a Nitrate-Reducing Strain of the Sulfate-Reducing Bacterium Desulfovibrio desulfuricans 27774

2009

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Desulfovibrio desulfuricans strain 27774 is one of a relative small group of sulfate-reducing bacteria that can also grow with nitrate as an alternative electron acceptor, but how nitrate reduction is regulated in any sulfate-reducing bacterium is controversial. Strain 27774 grew more rapidly and to higher yields of biomass with nitrate than with sulfate or nitrite as the only electron acceptor. In the presence of both sulfate and nitrate, sulfate was used preferentially, even when cultures were continuously gassed with nitrogen and carbon dioxide to prevent sulfide inhibition of nitrate reduction. The napC transcription start site was identified 112 bases upstream of the first base of the translation start codon. Transcripts initiated at the napC promoter that were extended across the napM-napA boundary were detected by reverse transcription-PCR, confirming that the six nap genes can be cotranscribed as a single operon. Real-time PCR experiments confirmed that nap operon expression is regulated at the level of mRNA transcription by at least two mechanisms: nitrate induction and sulfate repression. We speculate that three almost perfect inverted-repeat sequences located upstream of the transcription start site might be binding sites for one or more proteins of the CRP/FNR family of transcription factors that mediate nitrate induction and sulfate repression of nitrate reduction by D. desulfuricans.

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Section: Resultssupporting

confidence: 93%

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confidence: 99%

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confidence: 99%

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Preferential Reduction of the Thermodynamically Less Favorable Electron Acceptor, Sulfate, by a Nitrate-Reducing Strain of the Sulfate-Reducing Bacterium Desulfovibrio desulfuricans 27774

2009

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“…Nitrate and NO^ reduction are rather widespread in SO4 reducers (Keith and Herbert, 1983;McCready et al, 1983;Mitchell et al, 1986;Momaetal, 1997;Seitz and Cypionka, 1986), and in some cases NO^~ is preferred over SO|~ (Seitz and Cypionka, 1986). The bisulfite reductase enzyme in SO4 reducers displays some activity toward N0 2 , which partially explains the ubiquity of NOJT reduction.…”

Section: Overview Of Sulfate Reductionmentioning

confidence: 99%

Anaerobic Metabolism: Linkages to Trace Gases and Aerobic Processes

Megonigal¹,

Hines²,

Visscher³

2014

Treatise on Geochemistry

128

164

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“…Owing to its physiological importance, the twoelectron reduction step of nitrate to nitrite and the corresponding enzymes have been extensively studied. However, the capacity to use nitrate has been discovered only in a few sulfate-reducing bacteria including Desulfovibrio (Senez & Pichinoty, 1958;Liu & Peck, 1981;McCready et al, 1983;Seitz & Cypionka, 1986;Keith & Herbert, 1983;Mitchell et al, 1986), Desulfobulbus (Widdel & Pfenning, 1982), Desulfomonas (Widdel & Pfenning, 1984) and Desulfobacterium (Szewzyk & Pfenning, 1987). Nitrate is reduced to ammonia in a respiratory process coupled to electrontransport phosphorylation (Seitz & Cypionka, 1986).…”

Section: Introductionmentioning

confidence: 99%

Crystallization and preliminary X-ray analysis of a nitrate reductase from Desulfovibrio desulfuricans ATCC 27774

Dias¹,

Bursakov²,

Carneiro³

et al. 1999

Acta Crystallogr D Biol Cryst

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Periplasmic nitrate reductase from the sulfate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 contains two molybdopterin guanine dinucleotide cofactors and one [4Fe±4S] cluster as prosthetic groups and catalyzes the conversion of nitrate to nitrite. Crystals of the oxidized form of this enzyme were obtained using PEG as precipitant and belong to space group P3 1 21 or P3 2 21, with unit-cell dimensions a = b = 106.3, c = 135.1 A Ê . There is one monomer of 80 kDa in the asymmetric unit, which corresponds to a Matthews ratio of 2.75 A Ê 3 Da À1 . Using cryo-cooling procedures and X-rays from a rotating-anode generator, diffraction was observed to beyond 3.0 A Ê resolution.

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Respiratory nitrate reduction by Desulfovibrio sp.

Cited by 65 publications

References 22 publications

Preferential Reduction of the Thermodynamically Less Favorable Electron Acceptor, Sulfate, by a Nitrate-Reducing Strain of the Sulfate-Reducing Bacterium Desulfovibrio desulfuricans 27774

Preferential Reduction of the Thermodynamically Less Favorable Electron Acceptor, Sulfate, by a Nitrate-Reducing Strain of the Sulfate-Reducing Bacterium Desulfovibrio desulfuricans 27774

Anaerobic Metabolism: Linkages to Trace Gases and Aerobic Processes

Crystallization and preliminary X-ray analysis of a nitrate reductase from Desulfovibrio desulfuricans ATCC 27774

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