Respiratory Infection in the Rabbit: an Enzootic Caused by Pasteurella Lepiseptica and Attempts to Control it by Vaccination

Alexander, M.; Sawin, P. B.; Roehm, Dorothy Ann

doi:10.1093/infdis/90.1.30

Cited by 10 publications

(7 citation statements)

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“…One of the additionally encouraging methodologies is the improvement of vaccination techniques which over long stretches will ensure animals presented to P. multocida. Immunization investigations of rabbits with executed P. multocida have indicated variable outcomes [25][26][27]. By evaluation of erythrogram in the present study, vaccinated challenged rabbits of group III showed a significant decrease in RBCs count, hemoglobin concentration and PCV, associated with normal MCV and MCHC leading to normocytic normochromic anemia at the end of the 2 nd and 4 th week of challenging.…”

Section: Discussionmentioning

confidence: 54%

The Clinicopathological Effects of the Double Immunization with Formalized Killed Vaccine against Pasteurella multocida Challenge in Rabbits

Mohamed

Hashem

Mahmoud

2019

Zagazig Veterinary Journal

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The disease caused by Pasteurella multocida is an extremely common and difficult issue of rabbits utilized for biomedical research. The vaccination techniques are dependably the most powerful preventive measures. In the present study, 30 New Zealand rabbits (1.5 kg average body weight and 6-8 weeks old) were divided into 3 groups, control group (I), challenged nonvaccinated group (II), and challenged double vaccinated group (III). 1 st dose was administered at the 1 st day followed by similar booster dose after 21 days. At the end of the 2 nd , 4 th , and 6 th weeks of the experiment, blood samples were collected from the ear vein for hematological, plasma, and serum examination. At the end of the 6 th week, the rabbits were anaesthetized and sacrificed to collect the tissue specimens from liver, kidneys, spleen, lungs and heart for histopathological study. The results showed that double immunization with killed vaccine of P. multocida increased the immune response of the animals and the leukocyte phagocytic activity against P. multocida and also improved the clinicopathological and histopathological findings. Taken together, our findings proved that double immunization with killed vaccine of P. multocida increased the phagocytic activity of the immune cells and the immune status of animals against infection.

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Section: Discussionmentioning

confidence: 54%

The Clinicopathological Effects of the Double Immunization with Formalized Killed Vaccine against Pasteurella multocida Challenge in Rabbits

Mohamed

Hashem

Mahmoud

2019

Zagazig Veterinary Journal

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“…One criterion used to evaluate the efficacy of a vaccine is whether the vaccine can prevent the colonization of challenge organisms. Sequential nasal swabs were collected, 1,2,5,8,12,17, and 21 days after challenge. The challenge organisms colonized nasal cavities of the four nonvaccinated-challenged rabbits and the nine vaccinated-challenged rabbits, beginning on day 1 after challenge and persisting until the rabbits died or were killed 14 or 21 days later.…”

Section: Resultsmentioning

confidence: 99%

“…One of the more promising approaches is the development of immunization procedures which over long periods will protect animals exposed to P. multocida. Vaccination studies of rabbits with killed P. multocida have shown variable results (2,3,5). Alexander et al (2) vaccinated rabbits with an autologous killed P. multocida isolate by repeated intravenous injections.…”

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confidence: 99%

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Protection of rabbits against experimental pasteurellosis by a streptomycin-dependent Pasteurella multocida serotype 3:A live mutant vaccine

Lu¹,

Pakes²

1981

Infect Immun

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Pasteurella multocida (serotype 3:A) was isolated from a rabbit with clinical signs of suppurative rhinitis. This P. multocida strain was mutagenized wih Nmethyl-N'-nitro-N-nitrosoguanidine to obtain a genetically stable streptomycindependent mutant, from which a live vaccine was prepared. Pasteurella-free rabbits were inoculated intranasally three times at weekly intervals and challenged intranasally with a virulent serotype 3:A rabbit P. multocida isolate 2 weeks after the third vaccination. The rabbits were killed 2 to 3 weeks later. The vaccine did not cause clinical disease, death, or gross or microscopic lesions. Furthermore, the vaccine protected the challenge rabbits from developing clinical disease, death, and gross lesions. However, mild focal lung lesions were noted in several of the vaccinated-challenged animals. In contrast, nonvaccinated-challenged rabbits developed pyrexia and anorexia. Furthermore, three of four of these rabbits died with severe gross lesions including pyothorax, suppurative pericarditis, and fibrinopurulent pneumonia. Microscopically, the four nonvaccinated rabbits had moderate to severe suppurative pneumonia and mild to moderate suppurative rhinitis, and two had mild tympanitis. The mutant vaccine did not appear to colonize the nasal cavities. The vaccine prevented the colonization of the virulent challenge organism in lungs, liver, spleen, genital tracts, and blood, but not the nasal cavities.

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“…Another approach that is promising is the development of immunization procedures that will protect animals exposed to P. multocida. Vaccination with killed P. multocida has produced poor protection against pasteurellosis in several animal species including rabbits (1,2,5). In contrast, live mutant vaccines have been shown to produce protection against P. multocida challenge (6,16).…”

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confidence: 99%

Protection of rabbits against experimental pasteurellosis by vaccination with a potassium thiocyanate extract of Pasteurella multocida

Ringler¹,

Peter²,

Chrisp³

et al. 1985

Infect Immun

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Antigens were extracted from a virulent isolate of Pasteurella multocida (serotype 3,12,15:D) with potassium thiocyanate, and a vaccine was prepared. Pasteurella-free rabbits were vaccinated intranasally and intraconjuctivally twice with a 2-week interval and challenged intranasally with the homologous P. multocida serotype 2 weeks after the second vaccination. The vaccinated rabbits produced serum immunoglobulin G and nasal mucosal immunoglobulin A against P. multocida. The vaccine protected the challenged rabbits against clinical disease and death; however, otitis media was not prevented, and microscopic inflammatory lesions were occasionally noted in the lungs and nasal turbinates. In contrast, nonvaccinated, challenged rabbits became febrile, dyspnic, depressed, and anorectic, and five of six died within 4 days of challenge with severe lesions including pneumonia, pleuritis, otitis media, and bacteremia. The vaccine prevented death and colonization of challenge organisms in the blood and lung, but did not prevent colonization of the middle ear. The vaccine alone did not cause clinical disease or gross lesions, but did produce microscopic pulmonary inflammatory lesions.

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Respiratory Infection in the Rabbit: an Enzootic Caused by Pasteurella Lepiseptica and Attempts to Control it by Vaccination

Cited by 10 publications

References 0 publications

The Clinicopathological Effects of the Double Immunization with Formalized Killed Vaccine against Pasteurella multocida Challenge in Rabbits

The Clinicopathological Effects of the Double Immunization with Formalized Killed Vaccine against Pasteurella multocida Challenge in Rabbits

Protection of rabbits against experimental pasteurellosis by a streptomycin-dependent Pasteurella multocida serotype 3:A live mutant vaccine

Protection of rabbits against experimental pasteurellosis by vaccination with a potassium thiocyanate extract of Pasteurella multocida

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