Resorbable bioscaffold for esophageal repair in a dog model

Badylak, Stephen F.; Meurling, S; Chen, Mike Y.; Spievack, Alan R.; Simmons-Byrd, Abby

doi:10.1053/jpsu.2000.7834

Cited by 267 publications

(237 citation statements)

References 44 publications

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“…Complete epithelialization of the graft seems to require at least 3 months; this time was longer than the results obtained by Lopes et al for cervical esophagoplasty in a rat model (34). In fact, the time required for good re-epithelialization of grafts differed in previous reports (5,32,35). This may be related to the animal model, defect area, or the degree of inflammatory reaction after implantation.…”

Section: Discussionmentioning

confidence: 70%

“…Therefore, seeking alternatively promising supplements or replacements has attracted much research interest. In recent years, great efforts have been made to develop new artificial esophageal substitutes by using various synthetic and natural biomaterials, such as silicone/collagen hybrid, polyglycolic acid, allogenic aorta, or other tissue engineering materials (2)(3)(4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

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Esophageal replacement by hydroxylated bacterial cellulose patch in a rabbit model

Zhu¹,

Liu²,

Qian³

et al. 2015

Turk J Med Sci

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Background/aim: To repair esophageal defects by hydroxylated and kombucha -synthesized bacterial cellulose (HKBC) patch in a rabbit model. Materials and methods:Semicircular esophageal defects 1 cm in length of the cervical esophagus were initially created in 18 Japanese big-ear rabbits and then repaired with HKBC patch grafts. The clinical outcomes including survival rate, weight change, food intake, and hematological and radiologic evaluation were observed. After X-ray evaluation, the rabbits were sacrificed sequentially at 1, 3, and 6 months for histopathologic analysis with light microscopy and scanning electron microscopy.Results: Survival rate during the first month was 88.9% (n = 16). Two rabbits died from anastomotic leakage during the entire follow-up. Postoperatively, feeding function and body weight were gradually restored in the surviving animals. No hematological abnormalities were found, and no obvious anastomotic leakage, stenosis, or obstruction was observed under X-ray examination. The histopathologic results showed a progressive regeneration of the esophagus in the graft area, where the neo-esophagus tissue had characteristics similar to native esophageal tissue after 3 months of surgery. Conclusion:HKBC is beneficial for esophageal tissue regeneration and may be a promising material for esophageal reconstruction.

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Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Esophageal replacement by hydroxylated bacterial cellulose patch in a rabbit model

Zhu¹,

Liu²,

Qian³

et al. 2015

Turk J Med Sci

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“…In fact, circumferential replacement of the esophagus with an acellular scaffold, systematically lead to the formation of a stenosis. [25][26][27] After patch esophagoplasty 28 and circumferential esophageal replacement, 29 the addition of muscle cells to the matrix reduces the local inflammatory response and enhances the tissue remodeling process. While most authors use smooth muscle cells, 16,28,30 we chose skeletal myoblast, because of their capacity to differentiate into striated muscle and to promote regeneration of damaged muscle fibers.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Development and Characterization of a Tissue-Engineered Conduit Resembling Esophageal Wall Using Human and Pig Skeletal Myoblast, Oral Epithelial Cells, and Biologic Scaffolds

Poghosyan

Gaujoux

Vanneaux

et al. 2013

Tissue Engineering Part A

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Introduction: Tissue engineering represents a promising approach for esophageal replacement, considering the complexity and drawbacks of conventional techniques. Aim: To create the components necessary to reconstruct in vitro or in vivo an esophageal wall, we analyzed the feasibility and the optimal conditions of human and pig skeletal myoblast (HSM and PSM) and porcine oral epithelial cell (OEC) culture on biologic scaffolds. Materials and Methods: PSM and HSM were isolated from striated muscle and porcine OECs were extracted from oral mucosa biopsies. Myoblasts were seeded on an acellular scaffold issue from porcine small intestinal submucosa (SIS) and OEC on decellularized human amniotic membrane (HAM). Seeding conditions (cell concentrations [0.5 · 10 6 versus 10 6 cells/cm 2 ] and culture periods [7, 14 and 21 days]), were analyzed using the methyl thiazoltetrazolium assay, quantitative PCR, flow cytometry, and immunohistochemistry. Results: Phenotypic stability was observed after cellular expansion for PSM and HSM (85% and 97% CD56-positive cells, respectively), and OECs (90% AE1/AE3-positive cells). After PSM and HSM seeding, quantities of viable cells were similar whatever the initial cell concentration used and remained stable at all time points. During cell culture on SIS, a decrease of CD56-positive cells was observed (76% and 76% by D7, 56% and 70% by D14, 28% and 60% by D21, for PSM and HSM, respectively). Multilayered surface of a-actin smooth muscle and Desmine-positive cells organized in bundles was seen as soon as D7, with no evidence of cell within the SIS. Myoblasts fusion was observed at D21. Pax3 and Pax7 expression was downregulated and MyoD expression upregulated, at D14.OEC proliferation was observed on HAM with both cell concentrations from D7 to D21. The cell metabolism activity was more important on matrix seeded by 10 6 cells/cm 2 . With 0.5 · 10 6 OEC/cm 2

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“…More recently, also natural materials like collagen sponge or acellular matrix have been tested for their ability either to support healing of an intestinal wall damage or to entirely substitute a full thickness defect. Implantation of patches of these natural biomaterials yielded good results with respect to histological and, to some extent, even functional reconstitution Wang et al, 2003;Demirbilek et al, 2003;Badylak, S. et al, 2000;Isch et al, 2001;Kajitani et al, 2001;Mutter et al, 1996). As far as concerns the interposition of a synthetic or natural scaffold of tubular shape into esophagus or small intestine, results are less promising.…”

Section: Introductionmentioning

confidence: 99%

Tissue Engineering for Tissue and Organ Regeneration

Eberli¹

2011

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Resorbable bioscaffold for esophageal repair in a dog model

Cited by 267 publications

References 44 publications

Esophageal replacement by hydroxylated bacterial cellulose patch in a rabbit model

Esophageal replacement by hydroxylated bacterial cellulose patch in a rabbit model

In Vitro Development and Characterization of a Tissue-Engineered Conduit Resembling Esophageal Wall Using Human and Pig Skeletal Myoblast, Oral Epithelial Cells, and Biologic Scaffolds

Tissue Engineering for Tissue and Organ Regeneration

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