Esophageal replacement by hydroxylated bacterial cellulose patch in a rabbit model

Abstract

Background/aim: To repair esophageal defects by hydroxylated and kombucha -synthesized bacterial cellulose (HKBC) patch in a rabbit model. Materials and methods:Semicircular esophageal defects 1 cm in length of the cervical esophagus were initially created in 18 Japanese big-ear rabbits and then repaired with HKBC patch grafts. The clinical outcomes including survival rate, weight change, food intake, and hematological and radiologic evaluation were observed. After X-ray evaluation, the rabbits were sacrificed sequentially at 1, 3, and 6 months for histopathologic analysis with light microscopy and scanning electron microscopy.Results: Survival rate during the first month was 88.9% (n = 16). Two rabbits died from anastomotic leakage during the entire follow-up. Postoperatively, feeding function and body weight were gradually restored in the surviving animals. No hematological abnormalities were found, and no obvious anastomotic leakage, stenosis, or obstruction was observed under X-ray examination. The histopathologic results showed a progressive regeneration of the esophagus in the graft area, where the neo-esophagus tissue had characteristics similar to native esophageal tissue after 3 months of surgery. Conclusion:HKBC is beneficial for esophageal tissue regeneration and may be a promising material for esophageal reconstruction.

Applications of bacterial-synthesized cellulose in veterinary medicine – a review

“…Different disease, congenital or acquired (esophageal stricture, tumours) can lead to surgical esophagus resection that needs reconstruction using scaffolds (Zhu et al 2015a;Lua et al 2016). Current strategies and methods involve high costs and increased risks (Lua et al 2016).…”

“…Current strategies and methods involve high costs and increased risks (Lua et al 2016). The small or large intestine and autologous stomach have been extensively used (L v et al 2015;Zhu et al 2015a). However, their applicability is limited because of high morbidity or postsurgical complications (L v et al 2015;Zhu et al 2015b;Lua et al 2016).…”

“…However, their applicability is limited because of high morbidity or postsurgical complications (L v et al 2015;Zhu et al 2015b;Lua et al 2016). Recently, efforts were made to develop new substitutes using several natural or synthetic materials: silicone/collagen, allogenic aorta (Zhu et al 2015b) endoscopic balloon dilatation (Lua et al 2016) or other tissue engineering materials (Zhu et al 2015a;Lua et al 2016).…”

“…The ability of hydroxylated and kombucha-bacterial synthesized cellulose (HKBC) to repair esophageal defects was tested in rabbit model (Zhu et al 2015a). The defects were performed in the cervical esophagus and repaired with HKBC grafts.…”

Bacterial nanocelullose in biomedical applications: a review

“…12 It is worth mentioning that the 3D scaffolding and the grafted matrix were better in the regeneration of the epithelium 3 months after implantation. 12 In tissue engineering BNC is widely used in bone tissue engineering, [78][79][80] cartilage regeneration, 81 medicalization of the larynx, 82 repair of oesophageal defects 83 and many other applications. 12 Due to the fact that BNC has a high potential for use in tissue engineering one can expect that it will be used very widely in the future.…”