Applications of bacterial-synthesized cellulose in veterinary medicine – a review

Bodea, Ioana M.; Cătunescu, Giorgiana M.; Stroe, Teodor; Dîrlea, Sonia Alexandra; Beteg, F.

doi:10.2754/avb201988040451

Cited by 17 publications

(12 citation statements)

References 80 publications

(260 reference statements)

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“…The surface morphology of the BCd and BCm membranes as seen by SEM is shown in Supplementary data Figure 2 and Figure 3. The structure is a wellorganized, highly porous, three-dimensional brillar network, similar to previous reports (Alonso et al 2018;Bodea et al 2019;Pourjavaher et al 2017;Wu et al 2018). The matrix of BC membranes consists of randomly arranged nano bers and empty spaces distributed randomly in-between.…”

Section: Scanning Electron Microscopy (Sem)supporting

confidence: 89%

“…BC is a hydrogel-like membrane, and its brous and network-like structure contribute to its very high water content (Gea et al 2011). This property is important when considering its biomedical application as wound dressing material (Bodea et al 2019;Fu et al 2013).…”

Section: Film Weight and Yieldmentioning

confidence: 99%

“…By setting optimization goals in line with the requirements of a "wound dressing"-use scenario for BC, we obtained and validated the following values for the fermentation parameters: an inoculum volume of 5 mL and a harvest day of 16 for BCm, and an inoculum volume of 4 mL and a harvest day of 14 for BCd. However, of the 2 types, we would recommend the moist type BC because we managed to validate its production methods for all the 5 response characteristics, but also because its superior mechanical properties, a unique brillar structure and water uptake and release properties that makes it suitable for wound dressing (Bodea et al 2019;Fu et al 2013;Lin et al 2013)…”

Section: Optimization Of Bacterial Cellulose (Bc) By Response Surfacementioning

confidence: 99%

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Optimization of moist and oven-dried bacterial cellulose production for functional properties

Bodea

Beteg

Pop

et al. 2021

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Bacterial cellulose (BC) is a natural polymer with properties suitable for tissue engineering and possible applications in scaffold production. However, current procedures have limitations in obtaining BC pellicles with the desired structural, physical, and mechanical properties. Thus, this study analyzed the optimal culture conditions of BC membranes and 2 types of processing: draining and oven-drying. The aim was to obtain BC membranes with properties suitable for a wound dressing material. Two studies were carried out. In the preliminary study the medium (100 mL) was inoculated with varying volumes (1; 2; 3; 4; and 5 mL) and incubated statically for different periods (3; 6; 9; 12; and 18 days), using a full factorial experimental design. Thickness, uniformity, weight, and yield were evaluated. In the optimization study, a Box–Behnken design was used. Two independent variables were used: inoculum volume (X1: 1; 3; and 5 mL) and fermentation period (X2: 6; 12; and 18 d) to determine the target response variables: thickness, swelling ratio, drug release, fiber diameter, Tensile strength, and Young's Modulus for both dry and moist BC membranes. The mathematical modelling of the effect of the 2 independent variables was accomplished by response surface methodology (RSM). The obtained models were validated with new experimental values, and confirmed for all tested properties, except Young Modulus of oven-dried BC. Thus, the optimal properties in terms of a scaffold material of the moist BC were obtained with an inoculum volume of 5% (v/v) and 16 d of fermentation. While, for the oven-dried membranes a 4% (v/v) and 14 d of fermentation.

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Section: Scanning Electron Microscopy (Sem)supporting

confidence: 89%

Section: Film Weight and Yieldmentioning

confidence: 99%

Section: Optimization Of Bacterial Cellulose (Bc) By Response Surfacementioning

confidence: 99%

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Optimization of moist and oven-dried bacterial cellulose production for functional properties

Bodea

Beteg

Pop

et al. 2021

Preprint

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“…Bacterial cellulose (BC) has an array of physical, mechanical, and biological properties, such as swelling behavior [ 1 , 2 ], high tensile strength [ 1 , 3 ], high water-holding capability [ 2 ], high porosity [ 4 ], ultra-fine fiber network [ 1 ], biodegradability, non-toxic nature, and biocompatibility, which makes it a promising material in tissue engineering [ 5 , 6 , 7 , 8 ]. It is produced by several bacteria as a primary metabolic product in the form of swollen membranes in static culture [ 5 ], but also as irregular shape sphere-like cellulose suspensions, in agitated/shaking and bioreactor cultures [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Moist and Oven-Dried Bacterial Cellulose Production for Functional Properties

et al. 2021

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Bacterial cellulose (BC) is a natural polymer with properties suitable for tissue engineering and possible applications in scaffold production. However, current procedures have limitations in obtaining BC pellicles with the desired structural, physical, and mechanical properties. Thus, this study analyzed the optimal culture conditions of BC membranes and two types of processing: draining and oven-drying. The aim was to obtain BC membranes with properties suitable for a wound dressing material. Two studies were carried out. In the preliminary study, the medium (100 mL) was inoculated with varying volumes (1, 2, 3, 4, and 5 mL) and incubated statically for different periods (3, 6, 9, 12, and 18 days), using a full factorial experimental design. Thickness, uniformity, weight, and yield were evaluated. In the optimization study, a Box–Behnken design was used. Two independent variables were used: inoculum volume (X1: 1, 3, and 5 mL) and fermentation period (X2: 6, 12, and 18 d) to determine the target response variables: thickness, swelling ratio, drug release, fiber diameter, tensile strength, and Young’s modulus for both dry and moist BC membranes. The mathematical modelling of the effect of the two independent variables was performed by response surface methodology (RSM). The obtained models were validated with new experimental values and confirmed for all tested properties, except Young’s modulus of oven-dried BC. Thus, the optimal properties in terms of a scaffold material of the moist BC were obtained with an inoculum volume of 5% (v/v) and 16 d of fermentation. While, for the oven-dried membranes, optimal properties were obtained with a 4% (v/v) and 14 d of fermentation.

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“…Scaffolds embedded with antimicrobial agents, antibiotics, or several forms of silver nanoparticles, which are known antimicrobial agents, are attracting great interest in biomedical research. Metallic silver and silver nanoparticles (AgNPs) have been reported to provide a wide variety of antimicrobial activities [12,[26][27][28].…”

Section: Introductionmentioning

confidence: 99%

In Situ and Ex Situ Designed Hydroxyapatite: Bacterial Cellulose Materials with Biomedical Applications

et al. 2020

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Hydroxyapatite (HAp) and bacterial cellulose (BC) composite materials represent a promising approach for tissue engineering due to their excellent biocompatibility and bioactivity. This paper presents the synthesis and characterization of two types of materials based on HAp and BC, with antibacterial properties provided by silver nanoparticles (AgNPs). The composite materials were obtained following two routes: (1) HAp was obtained in situ directly in the BC matrix containing different amounts of AgNPs by the coprecipitation method, and (2) HAp was first obtained separately using the coprecipitation method, then combined with BC containing different amounts of AgNPs by ultrasound exposure. The obtained materials were characterized by means of XRD, SEM, and FT-IR, while their antimicrobial effect was evaluated against Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Staphylococcus aureus), and yeast (Candida albicans). The results demonstrated that the obtained composite materials were characterized by a homogenous porous structure and high water absorption capacity (more than 1000% w/w). These materials also possessed low degradation rates (<5% in simulated body fluid (SBF) at 37 °C) and considerable antimicrobial effect due to silver nanoparticles (10–70 nm) embedded in the polymer matrix. These properties could be finetuned by adjusting the content of AgNPs and the synthesis route. The samples prepared using the in situ route had a wider porosity range and better homogeneity.

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Applications of bacterial-synthesized cellulose in veterinary medicine – a review

Cited by 17 publications

References 80 publications

Optimization of moist and oven-dried bacterial cellulose production for functional properties

Optimization of moist and oven-dried bacterial cellulose production for functional properties

Optimization of Moist and Oven-Dried Bacterial Cellulose Production for Functional Properties

In Situ and Ex Situ Designed Hydroxyapatite: Bacterial Cellulose Materials with Biomedical Applications

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