Resonance scattering spectroscopy of gold nanoparticle

Jiang, Zhiliang; Feng, Z. G.; Li, Tingsheng; Li, Fang; Zhong, Fang; Xie, Jing; Yi, Xianghui

doi:10.1007/bf02879535

Cited by 51 publications

(32 citation statements)

References 8 publications

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“…This colloidal gold had an average particle size of 13.9 nm and a polydispersity index of 0.222 as measured by laser particle size analyzer. The concentration of the AuNPs was calculated using an established method [26] to be approximately 3.0 nmol/L, assuming that all gold in the HAuCl 4 was reduced.…”

Section: Preparation Of Aunpsmentioning

confidence: 99%

Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin

Shi

Xie

et al. 2012

Chin. Sci. Bull.

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The interactions between bovine serum albumin (BSA) and gold nanoparticles (AuNPs), and the conformational changes of BSA induced by this interaction, were investigated by UV-visible absorption spectroscopy, fluorescence spectroscopy, and Fourier transform infrared in combination with attenuated total reflection spectroscopy (ATR-FTIR). The critical adsorption density for preventing AuNP aggregation in 0.1 mol/L phosphate buffered saline (pH 7.2) was 23 BSA molecules per gold particle or 3.8×1012 BSA molecules/cm 2 . BSA bound to the AuNPs with high affinity (binding constant K s =7.59×10 8 L/mol), and the intrinsic fluorescence of BSA was quenched by the AuNPs in accordance with the static quenching mechanism. Both fluorescence spectroscopy and ATR-FTIR showed that AuNPs induced conformational changes in BSA, which resulted in it becoming less compact and increased the polarity of the microenvironment around the tryptophan residue Trp-212.

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Section: Preparation Of Aunpsmentioning

confidence: 99%

Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin

Shi

Xie

et al. 2012

Chin. Sci. Bull.

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“…1), with the immune reaction continuing. Because of the correlation of RS intensity with the size of the gold particles (22,23 ), the intensity was greatly enhanced. The process is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…After 5 min, we added 0.10 mL of 100 g/L KCl solution; 2 h later, we diluted the solution with doubly distilled water to 3.0 mL. We then used immunoresonance scattering to determine the scattering intensity at 560 nm (23,24 ). At a pH Ͻ7.0, in the case of ApoAI, the addition of the antisera did not stabilize the gold nanoparticles (see Table 1 in the online Data Supplement).…”

Section: Preparation Of the Immuno-gold Probementioning

confidence: 99%

Gold-Labeled Nanoparticle-Based Immunoresonance Scattering Spectral Assay for Trace Apolipoprotein AI and Apolipoprotein B

et al. 2006

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“…Second, the size (d) can be obtained from the formula d = (260λ max -1.34 × 10 5 ) 1/2 , by measuring the maximal absorption wavelength (λ max ). 26 The λ max was controlled at about 516 nm. Third, the RS intensity at 580 nm (I 580 nm ) increased with the size of nanogold; the I 580 nm was controlled at 7.0 ± 2 for 19.32 μg/mL nanogold.…”

Section: Identification Of Nanogoldmentioning

confidence: 99%

Rapid Assay of Trace Immunoglobulin M by a New Immunonanogold Resonance Scattering Spectral Probe

et al. 2008

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Nanogold, 8 nm in size, was used to label goat antihuman immunoglobulin M (GIgM) to obtain a new immunonanogold resonance scattering (RS) probe (Au-GIgG) for quantitation of trace immunoglobulin M (IgM). The Au-GIgG combined with IgM to form nanogold-labeled immunocomplex causes the RS intensity at 580 nm to be enhanced, in pH 4.49 KH 2 PO 4 -Na 2 HPO 4 buffer and in the presence of polyethylene glycol 6000. The enhanced RS intensity at 580 nm (ΔI 580 nm ) is proportional to the IgM concentration in the range of 1.5 to 2000 ng/mL, with a lower detection limit of 0.98 ng/mL. The immunonanogold RS assay was used to assay IgM in serum samples, with sensitivity, selectivity, and simplicity. (Journal of Biomolecular Screening 2008:302-308)

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Resonance scattering spectroscopy of gold nanoparticle

Cited by 51 publications

References 8 publications

Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin

Spectroscopic investigation of the interactions between gold nanoparticles and bovine serum albumin

Gold-Labeled Nanoparticle-Based Immunoresonance Scattering Spectral Assay for Trace Apolipoprotein AI and Apolipoprotein B

Rapid Assay of Trace Immunoglobulin M by a New Immunonanogold Resonance Scattering Spectral Probe

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