Gold-Labeled Nanoparticle-Based Immunoresonance Scattering Spectral Assay for Trace Apolipoprotein AI and Apolipoprotein B

Jiang, Zhiliang; Sun, Shi‐Gang; Liang, Aihui; Huang, Wen-Xin; Qin, Aimiao

doi:10.1373/clinchem.2005.061176

Cited by 77 publications

(33 citation statements)

References 15 publications

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“…Based on the catalysis of laccase to H 2 O 2 -KI-cationic surfactant system, an enzyme-catalytic RS assay was proposed for the determining laccase. Based on good biocompatibility and strong RS effect of nanogold, and combined with immune and enzymatic reactions [11][12][13][14][15][16][17], some highly sensitive and highly selective RS assays have been developed. As we know, enzymatic-RS assay has not been reported.…”

Section: Introductionmentioning

confidence: 99%

A sensitive enzyme-catalytic nanogold-resonance scattering spectral assay for alkaline phosphate

Jiang

Liu

et al. 2011

Bioprocess Biosyst Eng

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In pH 8.9 Tris-HCl buffer solutions, alkaline phosphatase (ALP) catalyzed the hydrolysis of ascorbic acid 2-phosphate (AAP) substrate to form ascorbic acid. Then H(3)PO(4) was added to stop the enzymatic reaction and HAuCl(4) was used to react with ascorbic acid to generate gold nanoparticles that exhibited a resonance scattering (RS) peak at 600 nm. Under the selected conditions, when the activity of ALP increased, the formed ascorbic acid and gold nanoparticles also increased. Thus, the RS intensity at 600 nm enhanced linearly. The linear range was 0.06-22 U/L, with a detection limit of 0.03 U/L. The ALP in serum was analyzed, and the results were in agreement with those of the fluorescence method.

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Section: Introductionmentioning

confidence: 99%

A sensitive enzyme-catalytic nanogold-resonance scattering spectral assay for alkaline phosphate

Jiang

Liu

et al. 2011

Bioprocess Biosyst Eng

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“…[1][2][3]. Nanogold has novel optical properties such as surface plasmon resonance absorption and surface plasmon resonance scattering effect (also called resonance scattering effect, RS).…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive resonance scattering spectral assay for carcinoembryonic antigen using immunonanogold as probe and nanocatalyst of NH2OH–Cu(II) particle reaction

Liu

Wen

Liang

et al. 2010

Bioprocess Biosyst Eng

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Nanogold of 10 nm was used to label carcinoembryonic antigen antibody (CEAAb) to prepare a probe (Au-CEAAb) for carcinoembryonic antigen (CEA). In a Na₂HPO₄-NaH₂PO₄ buffer solution of pH 6.8, CEA reacted with Au-CEAAb to form a big Au-CEAAb-CEA immunocomplex that can be removed by centrifugation. The unreacted Au-CEAAb in the centrifugal supernatant exhibited catalytic effect on the Cu₂O particle reaction, and the Cu₂O particles displayed a resonance scattering (RS) peak at 602 nm. When CEA increased, the RS intensity at 602 nm decreased, and the decreased RS intensity (ΔI (602 nm)) was linear to CEA concentration (C (CEA)) in the range of 0.02-12 ng mL(-1), with the regression equation of ΔI (602 nm) = 27.1 C (CEA) + 3.3, correlation coefficient of 0.9978 and detection limit of 3 pg mL(-1) CEA. The proposed method was applied to detect CEA in real samples, with satisfactory results.

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“…Gold nanoparticles are an important marker that offers excellent prospects for biological sensing because of their unique optical properties. Recently, the RS effect of nanogold was investigated and was combined with nanogold-labelled immunoreaction to obtain a more highly sensitive, better selective and simple RS assay for antigenic proteins (22,23). To the best of our knowledge, immunoresonance scattering for the determination of hapten penicillin G has not been reported.…”

Section: Introductionmentioning

confidence: 99%

A sensitive and selective immuno‐nanogold resonance‐scattering spectral method for the determination of trace penicillin G

Jiang

Qin

2008

Luminescence

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A sensitive and selective immuno-nanogold resonance scattering spectral assay was developed for the determination of trace hapten penicillin G, based on the resonance scattering (RS) effect of the nanogold at 560 nm, and the nanogold-labelled immunoreaction took place in pH 5.4 phosphate citric acid buffer solutions and in the presence of polythylene glycol (PEG). The nanogold-labelled immunocomplex formed more and more with addition of penicillin G. The enhanced RS intensity at 560 nm Delta I(RS) was linear to the penicillin G concentration in the range 7.5-1700 ng/mL, with a detection limit of 0.78 ng/mL. The results indicate that the immunonanogold-labelled RS spectral assay has a high specificity and sensitivity for quantitative determination of penicillin G in raw milk samples.

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Gold-Labeled Nanoparticle-Based Immunoresonance Scattering Spectral Assay for Trace Apolipoprotein AI and Apolipoprotein B

Cited by 77 publications

References 15 publications

A sensitive enzyme-catalytic nanogold-resonance scattering spectral assay for alkaline phosphate

A sensitive enzyme-catalytic nanogold-resonance scattering spectral assay for alkaline phosphate

A highly sensitive resonance scattering spectral assay for carcinoembryonic antigen using immunonanogold as probe and nanocatalyst of NH2OH–Cu(II) particle reaction

A sensitive and selective immuno‐nanogold resonance‐scattering spectral method for the determination of trace penicillin G

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